浙江医学
浙江醫學
절강의학
ZHEJIANG MEDICAL JOURNAL
2013年
18期
1640-1643
,共4页
丁金旺%罗定存%叶柳青%邓甬川%王克义%张卧%潘钢%周美琪%秦卫民%蔡菊芳
丁金旺%囉定存%葉柳青%鄧甬川%王剋義%張臥%潘鋼%週美琪%秦衛民%蔡菊芳
정금왕%라정존%협류청%산용천%왕극의%장와%반강%주미기%진위민%채국방
乳腺癌%血清%TBX3%酶联免疫吸附试验%分子标记物
乳腺癌%血清%TBX3%酶聯免疫吸附試驗%分子標記物
유선암%혈청%TBX3%매련면역흡부시험%분자표기물
Breast cancer%Serum%T- box 3(TBX3)%Enzyme linked immunosorbent assay(ELISA)%Biomarker
目的探讨乳腺癌患者血清中TBX3的表达水平，探讨其与临床病理特征的关系。方法采用ELISA法检测142例乳腺癌患者、70例乳腺良性疾病患者和80例健康女性血清中TBX3的表达水平，并比较3者之间的差异，分析血清TBX3与乳腺癌患者年龄、月经状态、组织学分级、腋窝淋巴结及远处脏器转移、TNM分期以及雌激素受体（ER）、孕激素受体（PR）、人表皮生长因子受体2（Her-2）、细胞核增殖抗原（Ki-67）之间的相关性。结果142例乳腺癌患者血清TBX3水平为2.13（1.01-6.15）ng/ml，显著高于健康对照组[1.60（0.99-2.18）ng/ml]及良性对照组[1.58（0.71-3.18）ng/ml]，差异均有统计学意义（P＜0.05或0.01）。血清TBX3表达水平与乳腺癌患者年龄、月经无关，但随着乳腺癌组织学级别增加、TNM分期进展、出现腋窝淋巴结及远处脏器转移而显著升高。此外，ER阳性及Her-2过表达者血清TBX3表达水平较ER阴性及Her-2低表达者明显升高，差异均有统计学意义（均P＜0.05）。Ki-67≥14%者与Ki-67＜14%者、PR阳性者与PR阴性者之间血清TBX3表达水平的差异均无统计学意义（均P＞0.05）。结论血清TBX3在乳腺癌患者血清中高表达，且与乳腺癌组织学分级、TNM分期、腋窝淋巴结及远处脏器转移、ER及Her-2密切相关，提示血清TBX3可能可以作为乳腺癌临床诊断、预后判断及随访监测的分子标记物。
目的探討乳腺癌患者血清中TBX3的錶達水平，探討其與臨床病理特徵的關繫。方法採用ELISA法檢測142例乳腺癌患者、70例乳腺良性疾病患者和80例健康女性血清中TBX3的錶達水平，併比較3者之間的差異，分析血清TBX3與乳腺癌患者年齡、月經狀態、組織學分級、腋窩淋巴結及遠處髒器轉移、TNM分期以及雌激素受體（ER）、孕激素受體（PR）、人錶皮生長因子受體2（Her-2）、細胞覈增殖抗原（Ki-67）之間的相關性。結果142例乳腺癌患者血清TBX3水平為2.13（1.01-6.15）ng/ml，顯著高于健康對照組[1.60（0.99-2.18）ng/ml]及良性對照組[1.58（0.71-3.18）ng/ml]，差異均有統計學意義（P＜0.05或0.01）。血清TBX3錶達水平與乳腺癌患者年齡、月經無關，但隨著乳腺癌組織學級彆增加、TNM分期進展、齣現腋窩淋巴結及遠處髒器轉移而顯著升高。此外，ER暘性及Her-2過錶達者血清TBX3錶達水平較ER陰性及Her-2低錶達者明顯升高，差異均有統計學意義（均P＜0.05）。Ki-67≥14%者與Ki-67＜14%者、PR暘性者與PR陰性者之間血清TBX3錶達水平的差異均無統計學意義（均P＞0.05）。結論血清TBX3在乳腺癌患者血清中高錶達，且與乳腺癌組織學分級、TNM分期、腋窩淋巴結及遠處髒器轉移、ER及Her-2密切相關，提示血清TBX3可能可以作為乳腺癌臨床診斷、預後判斷及隨訪鑑測的分子標記物。
목적탐토유선암환자혈청중TBX3적표체수평，탐토기여림상병리특정적관계。방법채용ELISA법검측142례유선암환자、70례유선량성질병환자화80례건강녀성혈청중TBX3적표체수평，병비교3자지간적차이，분석혈청TBX3여유선암환자년령、월경상태、조직학분급、액와림파결급원처장기전이、TNM분기이급자격소수체（ER）、잉격소수체（PR）、인표피생장인자수체2（Her-2）、세포핵증식항원（Ki-67）지간적상관성。결과142례유선암환자혈청TBX3수평위2.13（1.01-6.15）ng/ml，현저고우건강대조조[1.60（0.99-2.18）ng/ml]급량성대조조[1.58（0.71-3.18）ng/ml]，차이균유통계학의의（P＜0.05혹0.01）。혈청TBX3표체수평여유선암환자년령、월경무관，단수착유선암조직학급별증가、TNM분기진전、출현액와림파결급원처장기전이이현저승고。차외，ER양성급Her-2과표체자혈청TBX3표체수평교ER음성급Her-2저표체자명현승고，차이균유통계학의의（균P＜0.05）。Ki-67≥14%자여Ki-67＜14%자、PR양성자여PR음성자지간혈청TBX3표체수평적차이균무통계학의의（균P＞0.05）。결론혈청TBX3재유선암환자혈청중고표체，차여유선암조직학분급、TNM분기、액와림파결급원처장기전이、ER급Her-2밀절상관，제시혈청TBX3가능가이작위유선암림상진단、예후판단급수방감측적분자표기물。
Objective To investigate serum T- box 3 (TBX3) levels in patients with breast cancer and its relationship with clinical and pathological characteristics. Methods Serum TBX3 levels were determined by Enzyme linked immunosorbent assay (ELISA) in 142 patients with breast cancer, 70 patients with benign breast lesions and 80 healthy subjects. The correlations of serum TBX3 level with age, menstruation status, histological grade, axil ary lymph node and distance metastasis, TNM staging and ER, PR, Ki- 67, Her- 2 levels were analyzed. Results The concentration of serum TBX3 was 2.13 (1.01- 6.15)ng/ml in pa-tients with breast cancer and significantly higher than those in healthy subjects [1.60(0.99- 2.18)ng/ml, P<0.01] and patients with benign breast lesions [1.58 (0.71- 3.18)ng/ml, P<0.05]. Serum TBX3 levels in breast cancer patients were positively correlated with the histological grading, TNM staging, axil ary lymph node and distance metastasis, but not correlated with the menstruation status and age of patients. Furthermore, serum TBX3 in ER positive and Her- 2 over- expressed patients were significantly higher than those in ER negative and Her- 2 low- expressed ones (P<0.05), while there was no significant difference in serum Tbx3 be-tween the PR positive and PR negative,Ki- 67 positive and Ki- 67 negative patients (P >0.05). Conclusion Serum TBX3 is over- expressed in breast cancer, which is significantly correlated with histological grade, axil ary lymph node and distance metastasis, TNM staging, ER and Her- 2 status, indicating that it may serve as a biomarker for breast cancer and have potential clinical applications.
