浙江医学
浙江醫學
절강의학
ZHEJIANG MEDICAL JOURNAL
2013年
18期
1637-1639,1691
,共4页
血浆循环DNA%原发性干燥综合征%系统性红斑狼疮%干燥综合征活动指数%干燥综合征损害指数%系统性红斑狼疮活动指数
血漿循環DNA%原髮性榦燥綜閤徵%繫統性紅斑狼瘡%榦燥綜閤徵活動指數%榦燥綜閤徵損害指數%繫統性紅斑狼瘡活動指數
혈장순배DNA%원발성간조종합정%계통성홍반랑창%간조종합정활동지수%간조종합정손해지수%계통성홍반랑창활동지수
Plasma circulating DNA%Primary Sjogren's Syndrome%Systemic lupus erythematosus%Sjogren's syn-drome disease activity index%Sjogren's syndrome disease damage index%SLE disease activity index
目的比较原发干燥综合征（pSS）、系统性红斑狼疮（SLE）患者外周血浆中循环DNA（cDNA）浓度的差异性，探讨cDNA浓度在pSS、SLE诊治中的意义。方法选用人类基因组管家基因甘油醛-3-磷酸脱氢酶（GAPDH）设计引物，测定pSS、SLE及健康对照组中血浆cDNA浓度，同时测定血沉（ESR）、C-反应蛋白（CRP）、免疫球蛋白（IgA、IgG、IgM）并评定不同疾病的活动指数和损害指数，分析血浆cDNA浓度与疾病活动性、相关参数的关系。结果 pSS组cDNA浓度[（368.24±196.68）ng/ml]明显高于SLE组[（244.51±164.10）ng/ml]及健康对照组[（24.56±16.76）ng/ml]，差异均有统计学意义（均P＜0.05）；pSS组中的cDNA浓度值与干燥综合征活动指数（SSDAI）呈正相关（P＜0.05），与干燥综合征损害指数（SSDDI）无相关性（P＞0.05）；SLE组中cDNA浓度值与SLE活动指数（SLEDAI）无相关性（P＞0.05）。结论 cDNA浓度可作为pSS与SLE鉴别指标之一，pSS患者cDNA浓度与SSDAI呈正相关，可作为pSS疾病活动性观察指标之一。
目的比較原髮榦燥綜閤徵（pSS）、繫統性紅斑狼瘡（SLE）患者外週血漿中循環DNA（cDNA）濃度的差異性，探討cDNA濃度在pSS、SLE診治中的意義。方法選用人類基因組管傢基因甘油醛-3-燐痠脫氫酶（GAPDH）設計引物，測定pSS、SLE及健康對照組中血漿cDNA濃度，同時測定血沉（ESR）、C-反應蛋白（CRP）、免疫毬蛋白（IgA、IgG、IgM）併評定不同疾病的活動指數和損害指數，分析血漿cDNA濃度與疾病活動性、相關參數的關繫。結果 pSS組cDNA濃度[（368.24±196.68）ng/ml]明顯高于SLE組[（244.51±164.10）ng/ml]及健康對照組[（24.56±16.76）ng/ml]，差異均有統計學意義（均P＜0.05）；pSS組中的cDNA濃度值與榦燥綜閤徵活動指數（SSDAI）呈正相關（P＜0.05），與榦燥綜閤徵損害指數（SSDDI）無相關性（P＞0.05）；SLE組中cDNA濃度值與SLE活動指數（SLEDAI）無相關性（P＞0.05）。結論 cDNA濃度可作為pSS與SLE鑒彆指標之一，pSS患者cDNA濃度與SSDAI呈正相關，可作為pSS疾病活動性觀察指標之一。
목적비교원발간조종합정（pSS）、계통성홍반랑창（SLE）환자외주혈장중순배DNA（cDNA）농도적차이성，탐토cDNA농도재pSS、SLE진치중적의의。방법선용인류기인조관가기인감유철-3-린산탈경매（GAPDH）설계인물，측정pSS、SLE급건강대조조중혈장cDNA농도，동시측정혈침（ESR）、C-반응단백（CRP）、면역구단백（IgA、IgG、IgM）병평정불동질병적활동지수화손해지수，분석혈장cDNA농도여질병활동성、상관삼수적관계。결과 pSS조cDNA농도[（368.24±196.68）ng/ml]명현고우SLE조[（244.51±164.10）ng/ml]급건강대조조[（24.56±16.76）ng/ml]，차이균유통계학의의（균P＜0.05）；pSS조중적cDNA농도치여간조종합정활동지수（SSDAI）정정상관（P＜0.05），여간조종합정손해지수（SSDDI）무상관성（P＞0.05）；SLE조중cDNA농도치여SLE활동지수（SLEDAI）무상관성（P＞0.05）。결론 cDNA농도가작위pSS여SLE감별지표지일，pSS환자cDNA농도여SSDAI정정상관，가작위pSS질병활동성관찰지표지일。
Objective To investigate the plasma circulating DNA (cDNA)levels in patients with primary Sjogren's syn-drome(pSS) and systemic lupus erythematosus(SLE), and its clinical significance. Methods The primer was designed by human genome housekeeper gene GAPDH. Levels of cDNA, erythrocyte sedimentation rate (ESR), C- reaction protein (CRP) and im-munoglobulin (IgA, IgG and IgM) in each group were detected. Meanwhile, the activity index and damage index of different dis-eases were assessed, and the relations between cDNA and disease activity were analyzed. Results The levels of plasma cDNA in pSS group (368.24±196.68ng/ml) were apparently higher than those in SLE group (244.51±164.10ng/ml) and normal control group (24.56±16.76ng/ml),and the differences were significant statistical y (both P<0.05).The levels of cDNA in each disease group were significantly higher than those in normal control group (both P<0.05).In pSS group:cDNA level was positively corre-lation with Sjogren's syndrome disease activity index (SSDAI)(P<0.05).But there was no correlation between cDNA concentration and Sjogren's syndrome disease damage index (SSDDI)(P>0.05). In SLE group:cDNA level did not correlate with SLE disease activity index(SLEDAI)(P>0.05). Conclusion The cDNA level may be a valuable indicator for distinguishing pSS from SLE.And it can be taken as one of the disease activity indices of pSS.
