浙江医学
浙江醫學
절강의학
ZHEJIANG MEDICAL JOURNAL
2013年
18期
1628-1631,1643
,共5页
彭勇%陈勇%邬秀娣%罗晶%张振%黄娴倩%干敏芝
彭勇%陳勇%鄔秀娣%囉晶%張振%黃嫻倩%榦敏芝
팽용%진용%오수제%라정%장진%황한천%간민지
类风湿性关节炎%骨关节炎%滑膜细胞%缺氧诱导因子- 1α%血管内皮生长因子
類風濕性關節炎%骨關節炎%滑膜細胞%缺氧誘導因子- 1α%血管內皮生長因子
류풍습성관절염%골관절염%활막세포%결양유도인자- 1α%혈관내피생장인자
Rheumatoid arthritis%Osteoarthritis%Synoviocytes%Hypoxia- inducible factor- 1α%Vascular endothelial growth factor
目的研究类风湿性关节炎（RA）患者滑膜细胞在体外常氧和缺氧培养条件下缺氧诱导因子-1α（HIF-1α）和血管内皮生长因子（VEGF）表达的差异性及意义。方法对RA患者及骨关节炎（OA）患者的关节滑膜细胞在体外常氧和缺氧条件下分别进行培养，根据培养条件分为常氧组、缺氧6h组及缺氧12h组；采用免疫印迹法分别检测各组成纤维样滑膜细胞的HIF-1α及VEGF的表达情况。结果与常氧组相比，缺氧6h及12h的RA和OA患者滑膜细胞的HIF-1α及VEGF的表达均显著增加，差异均有统计学意义（均P＜0.05）。同时RA和OA患者缺氧12h组滑膜细胞HIF-1α及VEGF的表达高于缺氧6h组，差异均有统计学意义（均P＜0.05）。RA和OA患者3组滑膜细胞的HIF-1α及VEGF的表达均呈正相关（均P＜0.05）。常氧组RA与OA患者滑膜细胞的HIF-1α及VEGF的表达均无统计学差异（均P＞0.05）；而缺氧6h和12h组中RA滑膜细胞HIF-1α及VEGF的表达均高于OA滑膜细胞，差异均有统计学意义（均P＜0.05）。结论在缺氧条件下RA患者滑膜细胞HIF-1α及VEGF表达显著升高且密切相关，可能缺氧-HIF-1α-VEGF这一信号途径在RA的发生、发展中起着重要作用。
目的研究類風濕性關節炎（RA）患者滑膜細胞在體外常氧和缺氧培養條件下缺氧誘導因子-1α（HIF-1α）和血管內皮生長因子（VEGF）錶達的差異性及意義。方法對RA患者及骨關節炎（OA）患者的關節滑膜細胞在體外常氧和缺氧條件下分彆進行培養，根據培養條件分為常氧組、缺氧6h組及缺氧12h組；採用免疫印跡法分彆檢測各組成纖維樣滑膜細胞的HIF-1α及VEGF的錶達情況。結果與常氧組相比，缺氧6h及12h的RA和OA患者滑膜細胞的HIF-1α及VEGF的錶達均顯著增加，差異均有統計學意義（均P＜0.05）。同時RA和OA患者缺氧12h組滑膜細胞HIF-1α及VEGF的錶達高于缺氧6h組，差異均有統計學意義（均P＜0.05）。RA和OA患者3組滑膜細胞的HIF-1α及VEGF的錶達均呈正相關（均P＜0.05）。常氧組RA與OA患者滑膜細胞的HIF-1α及VEGF的錶達均無統計學差異（均P＞0.05）；而缺氧6h和12h組中RA滑膜細胞HIF-1α及VEGF的錶達均高于OA滑膜細胞，差異均有統計學意義（均P＜0.05）。結論在缺氧條件下RA患者滑膜細胞HIF-1α及VEGF錶達顯著升高且密切相關，可能缺氧-HIF-1α-VEGF這一信號途徑在RA的髮生、髮展中起著重要作用。
목적연구류풍습성관절염（RA）환자활막세포재체외상양화결양배양조건하결양유도인자-1α（HIF-1α）화혈관내피생장인자（VEGF）표체적차이성급의의。방법대RA환자급골관절염（OA）환자적관절활막세포재체외상양화결양조건하분별진행배양，근거배양조건분위상양조、결양6h조급결양12h조；채용면역인적법분별검측각조성섬유양활막세포적HIF-1α급VEGF적표체정황。결과여상양조상비，결양6h급12h적RA화OA환자활막세포적HIF-1α급VEGF적표체균현저증가，차이균유통계학의의（균P＜0.05）。동시RA화OA환자결양12h조활막세포HIF-1α급VEGF적표체고우결양6h조，차이균유통계학의의（균P＜0.05）。RA화OA환자3조활막세포적HIF-1α급VEGF적표체균정정상관（균P＜0.05）。상양조RA여OA환자활막세포적HIF-1α급VEGF적표체균무통계학차이（균P＞0.05）；이결양6h화12h조중RA활막세포HIF-1α급VEGF적표체균고우OA활막세포，차이균유통계학의의（균P＜0.05）。결론재결양조건하RA환자활막세포HIF-1α급VEGF표체현저승고차밀절상관，가능결양-HIF-1α-VEGF저일신호도경재RA적발생、발전중기착중요작용。
Objective To investigate the expression of hypoxia- inducible factor- 1α (HIF- 1α) and vascular endothelial growth factor (VEGF) on fibroblast- like synoviocytes cells in rheumatoid arthritis and its correlation with normoxia and hypoxia. Methods Primarily cultured fibroblast- like synoviocytes cells from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) were exposed to normoxia for 6h or hypoxia for 6h and 12h. The expression of HIF- 1αand VEGF were detected by Western blot. Results The expressions of HIF- 1α and VEGF in fibroblast- like synoviocytes cells from RA or OA patients exposed to hypoxia for 6h and 12h were higher than those exposed to normoxia(P<0.05). Compared to those exposed to hypoxia for 6h, the expres-sion of HIF- 1α and VEGF in cells exposed to hypoxia for 12h was higher (P<0.05). The expression of HIF- 1α was positively correlated with VEGF in three groups (P<0.05). The expressions of HIF- 1αand VEGF in fibroblast- like synoviocytes cells from RA patients exposed to hypoxia for 6h and 12h were higher than those from OA patients correspondingly (P<0.05), however, there were no significant difference in normoxic group between RA and OA patients(P>0.05). Conclusion In hypoxia conditions the expressions of HIF- 1α and VEGF on fibroblast- like synoviocytes cells from RA patients are significantly higher than those from OA patients, indicating that Hypoxia-HIF- 1α-VEGF signaling pathway may be closely related to the occurrence and de-velopment of RA.
