CAJ | 학술논문

为研究纤维素分解菌对奶牛瘤胃发酵的影响，选用3头体况良好，体重、日产奶量均相近，安装有永久性瘤胃瘘管的泌乳中期荷斯坦奶牛，采用3×3拉丁方试验设计，饲喂3种不同处理的日粮，纤维分解菌饲喂量分别为0（对照组）、0.7×1011 cfu/d（试验1组）和1.4×1011 cfu/d（试验2组）。试验分为3期，每期预试期15 d，正式期3 d。饲喂纤维分解菌后，测定瘤胃内pH、原虫数量、细菌总数、氨态氮浓度（NH3-N）、挥发性脂肪酸浓度（VFA）、菌体蛋白浓度。结果显示：两试验组瘤胃液pH（试验1组：6.49；试验2组：6.56）、氨态氮（试验1组：14.11；试验2组：14.64）、挥发性脂肪酸浓度（试验2组：101.76）、乙酸浓度（试验1组：67.38；试验2组：68.31）与对照组（pH：6.43、氨态氮：12.89、挥发性脂肪酸浓度：96.7、乙酸浓度：64.42）相比均显著提高（P＜0.05），而瘤胃原虫数、丙酸浓度、丁酸浓度和乙/丙酸比值与对照组相比无显著差异（P＞0.05）。
위연구섬유소분해균대내우류위발효적영향，선용3두체황량호，체중、일산내량균상근，안장유영구성류위루관적비유중기하사탄내우，채용3×3랍정방시험설계，사위3충불동처리적일량，섬유분해균사위량분별위0（대조조）、0.7×1011 cfu/d（시험1조）화1.4×1011 cfu/d（시험2조）。시험분위3기，매기예시기15 d，정식기3 d。사위섬유분해균후，측정류위내pH、원충수량、세균총수、안태담농도（NH3-N）、휘발성지방산농도（VFA）、균체단백농도。결과현시：량시험조류위액pH（시험1조：6.49；시험2조：6.56）、안태담（시험1조：14.11；시험2조：14.64）、휘발성지방산농도（시험2조：101.76）、을산농도（시험1조：67.38；시험2조：68.31）여대조조（pH：6.43、안태담：12.89、휘발성지방산농도：96.7、을산농도：64.42）상비균현저제고（P＜0.05），이류위원충수、병산농도、정산농도화을/병산비치여대조조상비무현저차이（P＞0.05）。
This experiment was conducted to study the effect of cellulose decomposing bacteria on rumen fermenta-tion in dairy cow.Three Holstein cows with similar body weight,parity,milk yield and permanent rumen fistula were as-signed to 3 treatments with different levels of sodium sulfate:0(control),0.7 ×1011cfu/d(treat 1)and 1.4 ×1011cfu/d(treat 2) by 3 ×3 Latin square design.The experiment was divided into 3 periods and each period lasted for 18 d ,including 15 d pre-experiment and 3 d experiment.Rumen pH,the number of protozoa,total number of bacteria,the concentrations of am-moniacal nitrogen (NH3-N),volatile fatty acids (VFA)and bacterial crude protein (BCP)were determined.The results showed that:the rumen pH(treat 1:6.49;treat 2:6.56),NH3-N(treat 1:14.11;treat 2:14.64),VFA concentrations(treat 2:101.76) and acetic acid concentration(treat 1:67.38;treat 2:68.31)were significantly higher than those of the control(P < 0.05), while the number of protozoa,propionic acid,butyrate,propionate and acetate to propionate ratios wasn’t significantly af-fected(P>0.05).