CAJ | 학술논문

为解决油页岩在开采利用中有机物对地下水的污染问题，试验采用驯化培养的石油降解菌，对地下水中的有机污染物进行生物降解。在低温(10℃)、120 r/min条件下，通过室内试验，确定菌群降解地下水中有机污染物的最佳pH为7，接种量为5 mL（8×106个/mL），初始浓度为200 mg/L，辅助氮源为氯化铵(0.05%)，辅助磷源为磷酸二氢铵(0.05%)。在此最佳条件下，反应6天后总石油烃质量浓度为33.94 mg/L，菌群对总石油烃及COD的降解率分别为83.03%及67.58%。通过对降解过程的动力学研究分析可知，总石油烃初始浓度为20 mg/L时的降解符合一级动力学方程，50~300 mg/L时的降解符合零级动力学方程。
위해결유혈암재개채이용중유궤물대지하수적오염문제，시험채용순화배양적석유강해균，대지하수중적유궤오염물진행생물강해。재저온(10℃)、120 r/min조건하，통과실내시험，학정균군강해지하수중유궤오염물적최가pH위7，접충량위5 mL（8×106개/mL），초시농도위200 mg/L，보조담원위록화안(0.05%)，보조린원위린산이경안(0.05%)。재차최가조건하，반응6천후총석유경질량농도위33.94 mg/L，균군대총석유경급COD적강해솔분별위83.03%급67.58%。통과대강해과정적동역학연구분석가지，총석유경초시농도위20 mg/L시적강해부합일급동역학방정，50~300 mg/L시적강해부합령급동역학방정。
To solve the problem of organic pollutants product in groundwater during exploitation and utilization of oil shale, the article took the oil degrading strains after domestication and cultivation to research the bio-degradation of the organic pollutants. The indoor research conducted at low temperature (10℃ ) and at shaking speed of 120 r/min. The experimental results show that: when the initial concentration of 200 mg/L, the optimum condition of the strains using the organic pollutants in groundwater was at pH 7, for inoculation of 5 mL, used ammonium chloride(0.05%) as the best nitrogen source and potassium sulfate(0.05%) as the best source of phosphorus. After 6 days at the optimum condition the concentration of total petroleum hydrocarbon could reduce from 200 mg/L to 33.94 mg/L. By the strains the degradation rate of the total petroleum hydrocarbon and COD was 83.03% and 67.58%, respectively. The biodegradation was proved to be first-order kinetic equation by kinetic analysis of degradation process under the initial concentration of the total petroleum hydrocarbon at 20 mg/L, and the total petroleum hydrocarbon initial concentration between 50-300 mg/L degradation meet zero-order kinetic equation.