CAJ | 학술논문

为了研究葡萄籽原花青素(Procyanidin from Grape Seed，GSP)对大鼠脾脏淋巴细胞与肝细胞增殖与活化的影响，揭示GSP提高机体免疫性能的作用机理，为后续的试验分组提供理论依据。试验采用不同终浓度的 GSP 培养液[0.00（对照）、0.05、0.10、0.50、1.00、5.00、10.00、15.00]与大鼠脾脏淋巴细胞，[0.00（对照）、0.05、0.10、0.50、1.00、5.00、10.00、15.00、20.00、25.00μg/mL ]与大鼠肝细胞系 IAR-20共育。在12、24、48 h后，用酶标仪在490 nm处测得各孔的OD值。结果显示：（1）添加0.05~5.00μg/mL的GSP 促进淋巴细胞增殖，当浓度超过10.00μg/mL 将抑制淋巴细胞增殖。（2）添加0.05~10.00μg/mL 的GSP促进肝细胞增殖，当浓度超过20.00μg/mL 将抑制肝细胞增殖。（3）一定范围内，GSP对淋巴细胞和肝细胞的促增殖作用有浓度和时间依赖性。葡萄籽原花青素能够促进体外培养大鼠的脾脏淋巴细胞和肝细胞增殖活性，具有提高细胞抗氧化和细胞免疫的作用。
위료연구포도자원화청소(Procyanidin from Grape Seed，GSP)대대서비장림파세포여간세포증식여활화적영향，게시GSP제고궤체면역성능적작용궤리，위후속적시험분조제공이론의거。시험채용불동종농도적 GSP 배양액[0.00（대조）、0.05、0.10、0.50、1.00、5.00、10.00、15.00]여대서비장림파세포，[0.00（대조）、0.05、0.10、0.50、1.00、5.00、10.00、15.00、20.00、25.00μg/mL ]여대서간세포계 IAR-20공육。재12、24、48 h후，용매표의재490 nm처측득각공적OD치。결과현시：（1）첨가0.05~5.00μg/mL적GSP 촉진림파세포증식，당농도초과10.00μg/mL 장억제림파세포증식。（2）첨가0.05~10.00μg/mL 적GSP촉진간세포증식，당농도초과20.00μg/mL 장억제간세포증식。（3）일정범위내，GSP대림파세포화간세포적촉증식작용유농도화시간의뢰성。포도자원화청소능구촉진체외배양대서적비장림파세포화간세포증식활성，구유제고세포항양화화세포면역적작용。
To study of the effects of GSP (Procyanidin from Grape Seed) on the Splenic Lymphocytes and hepatocytes proliferation from Rat, investigate the mechanism to improve immune performance of rats, provide a theoretical basis for the follow-up test group. The experiments used a single factor design. The splenic lymphocytes were treated with different concentration of GSP [0.00 (control group), 0.05, 0.10, 0.50, 1.00, 5.00, 10.00, 15.00 μg/mL ] and the hepatocytes were treated with different concentration of GSP [0.00 (control group), 0.05, 0.10, 0.50, 1.00, 5.00, 10.00, 15.00, 20.00, 25.00 μg/mL ]. Procyanidins incubated with these cells to 12, 24,48 h, respectively. The plates were read at 490 nm. The results showed that: (1) for the lymphocytes of Splenic, the concentrates of GSP ranging from 0.05 μg/mL to 5.00 μg/mL had promoted effect to the lymphocytes proliferation, while the concentrates of GSP enhanced reach to 10.00 μg/mL, the proliferation of splenic lymphocytes was inhibited; (2) for the hepatocytes IAR-20, the concentrates of GSP ranging from 0.05 μg/mL to 10.00 μg/mL had promoted effect to the hepatocytes proliferation, while the concentrates of GSP enhanced reach to 10.00 μg/mL, the proliferation of hepatocytes was inhibited; (3) Within a certain range, GSP exhibited concentration and time dependent stimulative effects on the proliferative activity of splenic lymphocytes and hepatocytes. GSP could promote the spleen lymphocytes and liver cells proliferation which cultured in vitro from Rat. So GSP had promoted effect to cellular antioxidant ability and immune function of the cells.