CAJ | 학술논문

为明确陕西关中地区甘蓝型油菜白粉病的病原种类，通过显微观察以及分子鉴定方法，对引起陕西关中地区油菜白粉病的病原进行鉴定。病原菌分生孢子梗简单不分枝，分生孢子顶生，无色，椭圆形或桶柱形，未发现闭囊壳。采用rDNA ITS通用引物扩增了病菌ITS区，对其产物进行测序及系统发育分析，5株菌与Erysiphe cruciferarum聚在一个独立的分枝上，支持率为96%。结合形态学和系统发育分析，鉴定陕西关中地区甘蓝型油菜白粉菌病原为Erysiphe cruciferarum。
위명학협서관중지구감람형유채백분병적병원충류，통과현미관찰이급분자감정방법，대인기협서관중지구유채백분병적병원진행감정。병원균분생포자경간단불분지，분생포자정생，무색，타원형혹통주형，미발현폐낭각。채용rDNA ITS통용인물확증료병균ITS구，대기산물진행측서급계통발육분석，5주균여Erysiphe cruciferarum취재일개독립적분지상，지지솔위96%。결합형태학화계통발육분석，감정협서관중지구감람형유채백분균병원위Erysiphe cruciferarum。
In order to define species of powdery mildew pathogen on Brassica napus L. in Guanzhong area of Shaanxi Province. The pathogen causing powdery mildew on B. napus, was identified by morphological characterization and phylogenetic analysis. Conidiophores of pathogen were unbranched. Conidia were borne singly, hyphae, cylindrical, or ellipsoid-cylindrica. No chasmothecia were found. The ITS region of rDNA was amplified with universal primers and sequenced. The collected five strains and the two Erysiphe cruciferarum strains fell within a single clade with 96% bootstrap support by established the parsimony tree. Based on phylogenetic analysis and morphological characters of the anamorph, we identified the collected five strains as Erysiphe cruciferarum in Shaanxi Province.