CAJ | 학술논문

谷氨酰胺转胺酶具有催化蛋白质分子内和分子间的交联，蛋白质和氨基酸之间的连接以及蛋白质分子内谷氨酰胺基水解的功能，被广泛应用于食品、医药及纺织等领域。微生物来源的谷氨酰胺转胺酶具有非Ca2+依赖性和底物特异性低等特点，在研究开发中更为引人注目。本研究综述了微生物谷氨酰胺转胺酶基因表达研究方面的进展，归纳了微生物谷氨酰胺转胺酶的基因序列特点及酶学特性，分析了该酶的结构与功能之间的关系，指出基因工程技术生产谷氨酰胺转胺酶的优势及存在的问题。认为应该进一步加强谷氨酰胺转胺酶的表达策略研究以及分子改造研究，以期提高该酶的产量、催化活力及热稳定性等。
곡안선알전알매구유최화단백질분자내화분자간적교련，단백질화안기산지간적련접이급단백질분자내곡안선알기수해적공능，피엄범응용우식품、의약급방직등영역。미생물래원적곡안선알전알매구유비Ca2+의뢰성화저물특이성저등특점，재연구개발중경위인인주목。본연구종술료미생물곡안선알전알매기인표체연구방면적진전，귀납료미생물곡안선알전알매적기인서렬특점급매학특성，분석료해매적결구여공능지간적관계，지출기인공정기술생산곡안선알전알매적우세급존재적문제。인위응해진일보가강곡안선알전알매적표체책략연구이급분자개조연구，이기제고해매적산량、최화활력급열은정성등。
Transglutaminase can generate covalent cross-links between protein molecules or intramolecular covalent cross-links to change functional properties of proteins, and is applied in industries of food processing, medicine, cosmetic and textile. Microbial transglutaminase has aroused much more attention because of its calcium-independent and broader substrate specificity. This article summarized the gene expression of microbial transglutaminase, reviewed the gene characteristics and enzymatic characteristics, analyzed the relationship between crystal structures and function, and introduced the advantages and problems of microbial transglutaminase production using genetic engineering technique. In order to improve the yield of the enzyme, the activity and thermo-stability, the suggestions about expression strategies and molecular modification of microbial transglutaminase were also provided.