中国农学通报
中國農學通報
중국농학통보
CHINESE AGRICULTURAL SCIENCE BULLETIN
2013年
24期
11-16
,共6页
黄娟%陈成斌%杨庆文%徐志健%梁云涛%张万霞%梁世春
黃娟%陳成斌%楊慶文%徐誌健%樑雲濤%張萬霞%樑世春
황연%진성빈%양경문%서지건%량운도%장만하%량세춘
普通野生稻%遗传多样性%分子标记
普通野生稻%遺傳多樣性%分子標記
보통야생도%유전다양성%분자표기
O.rufipogon Griff%genetic diversity%molecular marker
为了对来自贵港市最大面积原生地新收集普通野生稻资源进行遗传多样性分析,并在此基础上进一步对居群内的个体进行区分。选择分布于水稻染色体组的25对微卫星(SSR)引物对来自贵港的349份材料进行多样分析和遗传结构研究。结果表明,所有个体平均等位基因数A=6,有效等位基因数Ae=2.23,期望杂合度He=0.44,实际杂合度Ho=0.40,香农指数I=0.88。该居群可分为4个亚群结构,并且90%以上个体可以得到区分。该群体遗传多样性丰富,SSR分子标记可以简单有效的区分群体内的大部分个体,提高了保护效率。
為瞭對來自貴港市最大麵積原生地新收集普通野生稻資源進行遺傳多樣性分析,併在此基礎上進一步對居群內的箇體進行區分。選擇分佈于水稻染色體組的25對微衛星(SSR)引物對來自貴港的349份材料進行多樣分析和遺傳結構研究。結果錶明,所有箇體平均等位基因數A=6,有效等位基因數Ae=2.23,期望雜閤度He=0.44,實際雜閤度Ho=0.40,香農指數I=0.88。該居群可分為4箇亞群結構,併且90%以上箇體可以得到區分。該群體遺傳多樣性豐富,SSR分子標記可以簡單有效的區分群體內的大部分箇體,提高瞭保護效率。
위료대래자귀항시최대면적원생지신수집보통야생도자원진행유전다양성분석,병재차기출상진일보대거군내적개체진행구분。선택분포우수도염색체조적25대미위성(SSR)인물대래자귀항적349빈재료진행다양분석화유전결구연구。결과표명,소유개체평균등위기인수A=6,유효등위기인수Ae=2.23,기망잡합도He=0.44,실제잡합도Ho=0.40,향농지수I=0.88。해거군가분위4개아군결구,병차90%이상개체가이득도구분。해군체유전다양성봉부,SSR분자표기가이간단유효적구분군체내적대부분개체,제고료보호효솔。
To estimate the genetic diversity of common wild rice from the Largest Original Habitat in Guigang, Guangxi and further to distinguish the individuals within the population. Twenty-five SSR loci distributed on the twelve chromosomes of rice were used to study the genetic diversity and structure of 349 wild rice individuals. The results showed that, the mean number of allele (A) was 6 and the effective number of allele (Ae) was 2.23. Expected heterozygosity (He) was 0.44, and observed heterozygosity (Ho) was 0.40 as well as the Shannon index (I) was 0.88. The population could be divided into four subsets structure, and more than 90% of individuals could be distinguished. It had abundant genetic diversity within the population and SSR molecular markers was the effective method to distinguish most of individuals in the group also to improve protection efficiency of wild rice.