CAJ | 학술논문

目的：研究丹红注射液对SMMC-7721肝癌细胞凋亡的作用。方法将0,2,4,8μL·mL-1丹红注射液分别作用人肝癌细胞系SMMC-7721,在显微镜下观察不同浓度丹红注射液对细胞形态的影响,选择2μL·mL-1作用后,0,6,12,24 h流式细胞仪检测细胞凋亡指数变化；以噻唑蓝( MTT)法检测不同浓度丹红注射液对SMMC-7721肝癌细胞增殖的影响；逆转录-聚合酶链反应( RT-PCR)方法检测p53、Bax、Bcl-2在含不同浓度药物的SMMC-7721细胞中表达。结果随着药物浓度增大,SMMC-7721的凋亡形态越明显,并且随作用时间的延长,细胞凋亡指数也增加；不同浓度丹红注射液对SMMC-7721细胞均有明显的剂量和时间依赖性；不同浓度药物可诱导SMMC-7721肝癌细胞细胞凋亡；随着丹红注射液浓度增加,Bcl-2在SMMC-7721细胞中的表达降低,p53和Bax的表达增高。结论丹红注射液能促进SMMC-7721细胞的凋亡,且具有一定的剂量和时间依赖性。
목적：연구단홍주사액대SMMC-7721간암세포조망적작용。방법장0,2,4,8μL·mL-1단홍주사액분별작용인간암세포계SMMC-7721,재현미경하관찰불동농도단홍주사액대세포형태적영향,선택2μL·mL-1작용후,0,6,12,24 h류식세포의검측세포조망지수변화；이새서람( MTT)법검측불동농도단홍주사액대SMMC-7721간암세포증식적영향；역전록-취합매련반응( RT-PCR)방법검측p53、Bax、Bcl-2재함불동농도약물적SMMC-7721세포중표체。결과수착약물농도증대,SMMC-7721적조망형태월명현,병차수작용시간적연장,세포조망지수야증가；불동농도단홍주사액대SMMC-7721세포균유명현적제량화시간의뢰성；불동농도약물가유도SMMC-7721간암세포세포조망；수착단홍주사액농도증가,Bcl-2재SMMC-7721세포중적표체강저,p53화Bax적표체증고。결론단홍주사액능촉진SMMC-7721세포적조망,차구유일정적제량화시간의뢰성。
Objective To investigate the effects of danhong injection on the apoptosis of SMMC-7721 heptoma cells.Methods The hepatocellular carcinoma cell line SMMC-7721 was incubated with different concentrations(0,2,4,8 μL·mL-1) of danhong injection, and cell morphology was studied under the microscope. The apoptosis index was detected by flow cytometry at 0,6,12,24 h after cultured with 2 μL·mL-1danhong injection. Cell proliferation was measured by MTT assay;Gene expressions of p53,Bax and Bcl-2 were detected by RT-PCR at different drug concentrations. Results The morphology of the hepatoma cells changed obviously,and the apoptosis index increased by danhong injection in a dose-dependant manner. The danhong injection decreased gene expressions of Bcl-2,and obviously increased p53 and Bax. Conclusion Danhong injection can dose-and time-dependently promote apoptosis of SMMC-7721 cells.