CAJ | 학술논문

目的通过SD大鼠阴道涂片观察，及时准确判断大鼠是否存在正常动情周期及动情周期各阶段持续时间，为进一步的药物去势及反向添加药物实验提供依据，提高实验准确性。方法对40只12周龄SD雌性大鼠连续15 d进行阴道涂片、镜检，观察阴道组织涂片中细胞的变化，并记录图像，进行分析。结果3只大鼠不存在明显动情周期，退出实验。大鼠一个动情周期约为（110.88±20.42）h，动情期持续约（12.24±3.36）h。结论阴道组织涂片法是有效区分大鼠动情周期的各个阶段的一种简便方法。根据阴道涂片判断各雌鼠所处的动情周期阶段，可以提高实验操作准确性。
목적통과SD대서음도도편관찰，급시준학판단대서시부존재정상동정주기급동정주기각계단지속시간，위진일보적약물거세급반향첨가약물실험제공의거，제고실험준학성。방법대40지12주령SD자성대서련속15 d진행음도도편、경검，관찰음도조직도편중세포적변화，병기록도상，진행분석。결과3지대서불존재명현동정주기，퇴출실험。대서일개동정주기약위（110.88±20.42）h，동정기지속약（12.24±3.36）h。결론음도조직도편법시유효구분대서동정주기적각개계단적일충간편방법。근거음도도편판단각자서소처적동정주기계단，가이제고실험조작준학성。
Objective Through the SD rats vaginal smear observation,timely and accurate judgment rats duration of the existence of the normal estrous cycle and the various stages of the estrous cycle and to add drug experiments provide a basis for further medical castration and reverse,improve the experimental accuracy sex. Methods 40 12-week-old female SD rats continuous vaginal smear 15 d microscopy to observe the changes of the cells in the vaginal tissue smear,and record the image analysis. Results 3 rats obvious estrous cycle exit experiments. An estrous cycle in rats is approximately(110.88 ± 20.42)h,estrus continued approximately (12.24±3.36)h. Conclusion The vaginal tissue smear is a simple way to effectively distinguish between the various stages of the rat estrous cycle. According to the vaginal smear judgment to determine estrous cycle stage of the females which can improve the accuracy of the experimental operation.