CAJ | 학술논문

背景：近期有文献报道了粒细胞集落刺激因子在脑梗死模型及急性脊髓损伤模型中的神经保护作用，但应用的动物模型均非击打模型，与人体脊髓损伤的病理生理过程存在一定差距。 　　目的：观察粒细胞集落刺激因子对Al en’s脊髓损伤大鼠模型运动功能恢复的影响。 　　方法：使用改良Al en’s法制作T10节段Wistar大鼠脊髓损伤撞击模型，随机分为2组，粒细胞集落刺激因子组应用粒细胞集落刺激因子治疗，vehicle组注射等剂量PBS。于造模后第1，7，14，21，28，35天分别应用BBB运动功能评分法和Rivlin斜板实验评估大鼠运动功能，造模后第7，14，21，28，35天使用网格步行实验评估大鼠四肢肌力。 　　结果与结论：所有大鼠造模后均出现后肢瘫痪症状。造模后第7，14，21，28，35天粒细胞集落刺激因子组BBB运动功能评分及Rivlin斜板实验评分高于vehicle组(P<0.05-0.01)，造模后第14，21，28，35天粒细胞集落刺激因子组网格行走实验错误数低于vehicle组(P<0.05-0.01)，结果显示，粒细胞集落刺激因子治疗后大鼠运动功能及四肢肌力恢复情况均优于vehicle组。提示粒细胞集落刺激因子疗法对脊髓损伤起到了积极的治疗效果。
배경：근기유문헌보도료립세포집락자격인자재뇌경사모형급급성척수손상모형중적신경보호작용，단응용적동물모형균비격타모형，여인체척수손상적병리생리과정존재일정차거。 　　목적：관찰립세포집락자격인자대Al en’s척수손상대서모형운동공능회복적영향。 　　방법：사용개량Al en’s법제작T10절단Wistar대서척수손상당격모형，수궤분위2조，립세포집락자격인자조응용립세포집락자격인자치료，vehicle조주사등제량PBS。우조모후제1，7，14，21，28，35천분별응용BBB운동공능평분법화Rivlin사판실험평고대서운동공능，조모후제7，14，21，28，35천사용망격보행실험평고대서사지기력。 　　결과여결론：소유대서조모후균출현후지탄탄증상。조모후제7，14，21，28，35천립세포집락자격인자조BBB운동공능평분급Rivlin사판실험평분고우vehicle조(P<0.05-0.01)，조모후제14，21，28，35천립세포집락자격인자조망격행주실험착오수저우vehicle조(P<0.05-0.01)，결과현시，립세포집락자격인자치료후대서운동공능급사지기력회복정황균우우vehicle조。제시립세포집락자격인자요법대척수손상기도료적겁적치료효과。
BACKGROUND:Recently, a neuroprotective effect of granulocyte colony-stimulating factor was reported in a model of cerebral infarction and a model of acute spinal cord injury. However, the applied animal model was not established by impact method, different from pathophysiological process of human. OBJECTIVE:To observe effects of granulocyte colony-stimulating factor on motor function in a rat model of spinal cord injury induced by Al en’s method. METHODS:Wistar rats were used to establish spinal cord injury at T 10 level using modified Al en’s method. They were randomly assigned to two groups, granulocyte colony-stimulating factor group, treated with granulocyte colony-stimulating factor, and vehicle group, treated with equal volume of PBS. The motor function was evaluated with Basso-Beattie-Bresnahan score and modified Rivlin loxotic plate test monitored at 1, 7, 14, 21, 28 and 35 days, and four-limb muscle strength was assessed using Grid walk test at 7, 14, 21, 28 and 35 days post-operatively. RESULTS AND CONCLUSION:Hind limbs paralysis occurred in al animals postoperatively. Scores of Basso-Beattie-Bresnahan and modified Rivlin loxotic plate test were greater in granulocyte colony-stimulating factor group compared with vehicle group at 7, 14, 21, 28 and 35 days (P<0.05-0.01);mean Grid walk test errors were less in granulocyte colony-stimulating factor group compared with vehicle group at 14, 21, 28 and 35 days (P<0.05-0.01). Results indicate that motor function and four-limb muscle strength were improved fol owing granulocyte colony-stimulating factor therapy compared with vehicle group, indicating that granulocyte colony-stimulating factor has a positive effect on spinal cord injury.