CAJ | 학술논문

背景：异丙肾上腺素注射能够促进啮齿类动物涎腺腺泡细胞增殖、肥大。然而，异丙肾上腺素注射后涎腺干/祖细胞的增殖能力目前仍不清楚。 　　目的：研究异丙肾上腺素腹腔注射对SD大鼠下颌下腺干/祖细胞激活、增殖能力。 　　方法：SD大鼠随机分为2组，分别腹腔注射异丙肾上腺素与生理盐水，连续注射5 d后取下颌下腺，酶消化法制取下颌下腺细胞悬液，体外培养，获得下颌下腺类上皮细胞集落并计数。挑取增殖较快的集落，进行CD90.1、层粘连蛋白、α6β1等免疫细胞化学检测。 　　结果与结论：与对照组相比，异丙肾上腺素注射组中、低增殖集落数量较少，高增殖集落数量较多，差异有显著性意义(P<0.05)，而集落总数无明显区别(P>0.05)。高增殖下颌下腺类上皮细胞集落CD90.1、层粘连蛋白、α6β1抗体均为阳性表达。实验结果显示，异丙肾上腺素注射不能明显增加下颌下腺中的干/祖细胞数量，但是能提高腺体中干/祖细胞的增殖能力。
배경：이병신상선소주사능구촉진교치류동물연선선포세포증식、비대。연이，이병신상선소주사후연선간/조세포적증식능력목전잉불청초。 　　목적：연구이병신상선소복강주사대SD대서하합하선간/조세포격활、증식능력。 　　방법：SD대서수궤분위2조，분별복강주사이병신상선소여생리염수，련속주사5 d후취하합하선，매소화법제취하합하선세포현액，체외배양，획득하합하선류상피세포집락병계수。도취증식교쾌적집락，진행CD90.1、층점련단백、α6β1등면역세포화학검측。 　　결과여결론：여대조조상비，이병신상선소주사조중、저증식집락수량교소，고증식집락수량교다，차이유현저성의의(P<0.05)，이집락총수무명현구별(P>0.05)。고증식하합하선류상피세포집락CD90.1、층점련단백、α6β1항체균위양성표체。실험결과현시，이병신상선소주사불능명현증가하합하선중적간/조세포수량，단시능제고선체중간/조세포적증식능력。
BACKGROUND:Injection of isoproterenol is known to induce proliferation and hypertrophy of acinar cells in rodent salivary glands. However, the clonal proliferation ability of stem/progenitor cells of salivary glands by isoproterenol remains unclear. OBJECTIVE:To study the proliferation and activation ability of stem/progenitor cells of submandibular gland with colony assay by intraperitoneal injection of isoproterenol. METHODS:Sprague-Dawley rats were randomly divided into two groups, isoproterenol and control groups, respectively intraperitonal y injected with isoproterenol and normal saline for 5 consecutive days. The gland tissues were harvested, and the stem/progenitor cells of submandibular gland were obtained by enzyme digestion in vitro. The number of clonal colonies of each group was analyzed. The larger colony cells were col ected for immunohistochemistry staining with CD90.1, laminin andα6β1. RESULTS AND CONCLUSION:The number of middle and low proliferative potential colony-forming cells was less but high proliferative potential colony forming cells were significantly more in isoproterenol group compared with control group (P<0.05). However, there was no significant difference in the total number of the colonies between two groups (P>0.05). The high proliferative potential colony forming cells were positive for CD90.1, laminin andα6β1. Results showed that isoproterenol treatment model cannot increase the cellnumber, but enhance the proliferation ability of stem/progenitor cells from the submandibular gland.