CAJ | 학술논문

背景：大量的研究报道证明滑膜间充质干细胞在细胞形态、免疫表型、集落形成能力和分化潜能等方面与骨髓间充质干细胞相似，但在向软骨分化的能力上，滑膜间充质干细胞明显优于骨髓间充质干细胞。目的：探讨滑膜间充质干细胞作为半月板软骨组织工程种子细胞的可行性。方法：通过有限稀释单克隆培养法将滑膜间充质干细胞从兔滑膜组织中分离出来并加以纯化，在体外培养条件下对其形态学、超微结构、分子表型、增殖动力学、核型以及致瘤性等进行分析。结果与结论：从兔滑膜细胞中分离纯化出滑膜间充质干细胞，体外单层培养具有极强的增殖能力，在第6天时达到生长的最高峰，倍增时间为(30.2±2.4) h。流式细胞术检测滑膜间充质干细胞表达间充质干细胞一些分子标记CD44、CD90。DNA含量检测、染色体核型分析、荷瘤实验结果表明，分离纯化的滑膜间充质干细胞是正常的二倍体细胞，无致瘤性，因此可作为半月板组织工程的种子细胞。
배경：대량적연구보도증명활막간충질간세포재세포형태、면역표형、집락형성능력화분화잠능등방면여골수간충질간세포상사，단재향연골분화적능력상，활막간충질간세포명현우우골수간충질간세포。목적：탐토활막간충질간세포작위반월판연골조직공정충자세포적가행성。방법：통과유한희석단극륭배양법장활막간충질간세포종토활막조직중분리출래병가이순화，재체외배양조건하대기형태학、초미결구、분자표형、증식동역학、핵형이급치류성등진행분석。결과여결론：종토활막세포중분리순화출활막간충질간세포，체외단층배양구유겁강적증식능력，재제6천시체도생장적최고봉，배증시간위(30.2±2.4) h。류식세포술검측활막간충질간세포표체간충질간세포일사분자표기CD44、CD90。DNA함량검측、염색체핵형분석、하류실험결과표명，분리순화적활막간충질간세포시정상적이배체세포，무치류성，인차가작위반월판조직공정적충자세포。
BACKGROUND:A large amount of studies have confirmed that synovial mesenchymal stem cells have the similarity in cellmorphology, immune phenotype, colony forming ability and differentiation potential with bone marrow mesenchymal stem cells. But bone marrow mesenchymal stem cells are better than synovial mesenchymal stem cells in the ability to differentiate into cartilages. OBJECTIVE:To discuss the possibility of using synovial mesenchymal stem cells as seed cells for meniscal tissue engineering. METHODS:The synovial mesenchymal stem cells were isolated from rabbit synovial tissues with limiting dilution monoclonal culture method, and then the cells were purified. The morphology, ultrastructure, molecular phenotype, proliferation kinetics, karyotype and tumorigenicity of the in vitro cultured cells were analyzed. RESULTS AND CONCLUSION:The synovial mesenchymal stem cells isolated from the rabbit synovial cells had high proliferation capacity during in vitro monolayer culture. The synovial mesenchymal stem cells grew to peak at 6 days, and the doubling time was (30.2±2.4) hours. Flow cytometry results showed the synovial mesenchymal stem cells could express some molecular makers of mesenchymal stem cells, such as CD44 and CD90. DNA contents check, karyotype test and oncogenicity test confirmed isolated and purified synovial mesenchymal stem cells were the normal diploid cells without tumorigenicity, so the cells can be used as seed cells for meniscal tissue engineering.