CAJ | 학술논문

背景：前期研究发现控释胶质细胞源性神经营养因子与骨髓间充质干细胞源神经元样细胞联合移植可有效促进猕猴脊髓损伤后运动功能和感觉功能的恢复。 　　目的：观察控释胶质细胞源性神经营养因子联合骨髓间充质干细胞源神经元样细胞移植抑制猴脊髓损伤后胶质瘢痕形成的作用是否优于单纯细胞移植。 　　方法：取12只恒河猴，采用改良Al en氏法制作急性重度脊髓损伤模型，随机数字表法分为3组，实验组以控释胶质细胞源性神经营养因子联合自体骨髓间充质干细胞源神经元样细胞移植修复，对照组以自体骨髓间充质干细胞源神经元样细胞移植修复，空白对照组以磷酸盐缓冲液修复。修复后5个月，取出脊髓组织制成石蜡标本，应用免疫组织化学染色显示胶质瘢痕的形态特征、构成特点及瘢痕中神经纤维的再生情况，检测胶质瘢痕面积及胶质纤维酸性蛋白染色的平均吸光度值。 　　结果与结论：脊髓损伤部位胶质瘢痕由混合性增生的星形胶质细胞和组织细胞构成。空白对照组脊髓胶质瘢痕累及范围广，星形胶质细胞增生显著，神经丝蛋白免疫组织化学染色阴性，胶质瘢痕面积、胶质纤维酸性蛋白染色平均吸光度值高于实验组与对照组(P<0.05)；实验组、对照组脊髓胶质瘢痕累及范围较局限，神经丝蛋白免疫组织化学染色显示有少量神经纤维通过瘢痕区，并且实验组胶质瘢痕面积、胶质纤维酸性蛋白染色平均吸光度值低于对照组(P<0.05)。结果表明，控释胶质细胞源性神经营养因子联合骨髓间充质干细胞源性神经元样细胞移植可更强抑制脊髓损伤后胶质瘢痕的形成。
배경：전기연구발현공석효질세포원성신경영양인자여골수간충질간세포원신경원양세포연합이식가유효촉진미후척수손상후운동공능화감각공능적회복。 　　목적：관찰공석효질세포원성신경영양인자연합골수간충질간세포원신경원양세포이식억제후척수손상후효질반흔형성적작용시부우우단순세포이식。 　　방법：취12지항하후，채용개량Al en씨법제작급성중도척수손상모형，수궤수자표법분위3조，실험조이공석효질세포원성신경영양인자연합자체골수간충질간세포원신경원양세포이식수복，대조조이자체골수간충질간세포원신경원양세포이식수복，공백대조조이린산염완충액수복。수복후5개월，취출척수조직제성석사표본，응용면역조직화학염색현시효질반흔적형태특정、구성특점급반흔중신경섬유적재생정황，검측효질반흔면적급효질섬유산성단백염색적평균흡광도치。 　　결과여결론：척수손상부위효질반흔유혼합성증생적성형효질세포화조직세포구성。공백대조조척수효질반흔루급범위엄，성형효질세포증생현저，신경사단백면역조직화학염색음성，효질반흔면적、효질섬유산성단백염색평균흡광도치고우실험조여대조조(P<0.05)；실험조、대조조척수효질반흔루급범위교국한，신경사단백면역조직화학염색현시유소량신경섬유통과반흔구，병차실험조효질반흔면적、효질섬유산성단백염색평균흡광도치저우대조조(P<0.05)。결과표명，공석효질세포원성신경영양인자연합골수간충질간세포원성신경원양세포이식가경강억제척수손상후효질반흔적형성。
BACKGROUND:Previous studies have demonstrated that transplantation of control ed release glial cellline-derived neurotrophic factor and bone marrow mesenchymal stem cells-derived neuron-like cells can effectively promote the motor function and sensory function recovery of rhesus monkeys with spinal cord injury. OBJECTIVE:To validate whether co-transplantation of control ed release glial cellline-derived neurotrophic factor and bone marrow mesenchymal stem cells-derived neuron-like cells exhibits better protective effects on spinal cord glial scar of rhesus monkeys with spinal cord injury than celltransplantation alone. METHODS:Twelve rhesus monkeys were col ected to prepare animal models of acute severe spinal cord injury using modified Al en’s method, and then randomly divided into three groups:experimental group, co-transplantation of control ed release glial cellline-derived neurotrophic factor and bone marrow mesenchymal stem cells-derived neuron-like cells;control group, simple transplantation of bone marrow mesenchymal stem cells-derived neuron-like cells;blank control group, PBS. After 5 months, paraffin specimens of the spinal cord were made for detection of morphological and compositional characteristics of glial scar, regeneration of nerve fibers in the scar, glial scar area, and average absorbance of glial fibril ary acidic protein. RESULTS AND CONCLUSION:Glial scar in the injured spinal cord was composed of astrocytes and histocytes. Less spinal cord glial scar area and lower absorbance value could be observed in the experimental and control groups as compared with the blank control group (P<0.05). In addition, in the blank control group, neurofilament negative fibers could be observed in the glial scar, and astrocytes proliferated obviously. The experimental and control groups showed less fibers passed through the scar area. The glial scar area and average absorbance in the experimental group was lower than that in the control group (P<0.05). These findings suggest that compared with simple transplantation of bone marrow mesenchymal stem cells-derived neuron-like cells, co-transplantation of control ed release glial cellline-derived neurotrophic factor and bone marrow mesenchymal stem cells-derived neuron-like cells shows better protective effects spinal tissue structure after spinal cord injury, which may be one of mechanisms by which the number of glial scars is reduced to a greater extent.