CAJ | 학술논문

背景：临床中发现腰椎间盘突出症常在一个家族中多人甚至全部发病，而且发病的部位、原因、症状基本一致，这表明基因在该种病症中扮演着重要的角色。目的：分析家族性腰椎椎间盘突出症患者椎间盘内Fas凋亡基因表达的特点。方法：用半定量RT-PCR方法检测15例家族性腰椎椎间盘突出患者、21例普通椎间盘突出患者、5例新鲜尸体椎间盘的软骨终板和髓核组织中Fas基因的表达情况。结果与结论：家族性椎间盘突出与普通椎间盘突出患者终板内Fas基因的表达均高于新鲜尸体椎间盘终板内Fas基因的表达，差异有显著性意义(P<0.05)，家族性椎间盘突出与普通椎间盘突出患者终板内Fas基因的表达差异无显著性意义(P>0.05)。家族性椎间盘突出患者髓核内Fas基因表达与普通椎间盘突出患者、新鲜尸体髓核内Fas基因表达相比较，差异无显著性意义(P>0.05)。结果可见家族性椎间盘终板中Fas基因的表达增加可能是继发性的，可以通过阻止终板退变来达到阻止椎间盘退变的目的。
배경：림상중발현요추간반돌출증상재일개가족중다인심지전부발병，이차발병적부위、원인、증상기본일치，저표명기인재해충병증중분연착중요적각색。목적：분석가족성요추추간반돌출증환자추간반내Fas조망기인표체적특점。방법：용반정량RT-PCR방법검측15례가족성요추추간반돌출환자、21례보통추간반돌출환자、5례신선시체추간반적연골종판화수핵조직중Fas기인적표체정황。결과여결론：가족성추간반돌출여보통추간반돌출환자종판내Fas기인적표체균고우신선시체추간반종판내Fas기인적표체，차이유현저성의의(P<0.05)，가족성추간반돌출여보통추간반돌출환자종판내Fas기인적표체차이무현저성의의(P>0.05)。가족성추간반돌출환자수핵내Fas기인표체여보통추간반돌출환자、신선시체수핵내Fas기인표체상비교，차이무현저성의의(P>0.05)。결과가견가족성추간반종판중Fas기인적표체증가가능시계발성적，가이통과조지종판퇴변래체도조지추간반퇴변적목적。
BACKGROUND:The clinical research have found that the interbervebral disc herniation often occurs in several members or even al the members of a family, and the location, reason and symptom are basical y the same, indicating that genes play an important role in this kind of disease. OBJECTIVE:To analyze the apoptosis Fas gene expression characteristics of lumbar disc in the familial patients with intervertebral disc herniation. METHODS:Semi-quantitative reverse transcription-PCR was used to test Fas gene expression of vertebral pulp and cartilage endplate in the intervertebral disc among 15 familial patients, 21 ordinary patients and five fresh cadavers. RESULTS AND CONCLUSION:Fas gene expression level of endplate of familial and ordinary patients with intervertebral disc herniation was higher than that of fresh cadavers, and there was no significant difference (P<0.05);there was no significant difference in Fas gene expression in endplates between familial patients and ordinary patients with intervertebral disc herniation (P>0.05). Compared with the vertebral pulps of ordinary patients with intervertebral disc herniation and fresh cadavers, there was no significant difference in the Fas expression of vertebral pulps of familial patients with intervertebral disc herniation (P>0.05). The increasing Fas gene expression may be secondary in the endplates of familial patients with intervertebral disc herniation, which can prevent intervertebral disc degeneration through preventing the endplate degeneration.