CAJ | 학술논문

背景：虽然国内与国外关于新等位基因的报道较多，但是，因某些等位基因首次发现较晚，而后却再次被检测到，并且等位基因频率很低，此类相关文章报道甚少，这些等位基因往往被忽视。目的：检测与确认4例临床移植配型供受者携带的罕见等位基因。方法：采用快速 DNA提取试剂盒从全血样本中提取基因组DNA，经人类白细胞抗原基因商品化测序分型试剂盒检测和SSP高分辨试剂盒验证，得到人类白细胞抗原高分辨分型结果。结果与结论：被检测到的4个罕见等位基因分别为B*27：15、B*51：39、C*07：66和C*08：22。对于以上4个等位基因虽被美国骨髓库列为罕见等位基因，但在中国可能并不罕见。事实证明，被美国骨髓库列为罕见等位基因的C*08：22为中国汉族人群常见等位基因。
배경：수연국내여국외관우신등위기인적보도교다，단시，인모사등위기인수차발현교만，이후각재차피검측도，병차등위기인빈솔흔저，차류상관문장보도심소，저사등위기인왕왕피홀시。목적：검측여학인4례림상이식배형공수자휴대적한견등위기인。방법：채용쾌속 DNA제취시제합종전혈양본중제취기인조DNA，경인류백세포항원기인상품화측서분형시제합검측화SSP고분변시제합험증，득도인류백세포항원고분변분형결과。결과여결론：피검측도적4개한견등위기인분별위B*27：15、B*51：39、C*07：66화C*08：22。대우이상4개등위기인수피미국골수고렬위한견등위기인，단재중국가능병불한견。사실증명，피미국골수고렬위한견등위기인적C*08：22위중국한족인군상견등위기인。
BACKGROUND:Many articles concerned novel al eles reported in China and outside China, but some al eles were detected lately. After that, these al eles were tested again. Because frequencies of these al eles are very low, few relevant articles are reported, so these al eles are ignored easily. OBJECTIVE:To test and identify four human leukocyte antigen rare al eles that patients and donors carries. METHODS:Genomic DNA was extracted automatical y from blood samples using quick DNA purified kit, typed by human leukocyte antigen locus commercial sequence-based typing kits and confirmed by sequence-specific primers high-resolution kits. RESULTS AND CONCLUSION:Four detected rare al eles are B*27:15, B*51:39, C*07:66 and C*08:22. The four above-mentioned human leukocyte antigen rare al eles defined by National Marrow Donor Program are not rare in China. The facts prove that C*08:22 which was defined a rare al ele by National Marrow Donor Program before is a common al ele in Chinese Han nationality.