CAJ | 학술논문

背景：由于关节软骨无神经和血管，其营养主要来源于滑液和滑膜血管的渗透作用，自身修复能力有限，因而如何更好的修复关节软骨损伤成为亟待解决的医学难题。目的：回顾近年来关于软骨损伤修复方法以及异体软骨体外保存方法的文献研究，找到适合异体软骨组织体外保存的最佳保存条件以及培养液介质成分，从而提高异体软骨组织体外保存效果。方法：计算机检索PubMed数据库和中国期刊全文数据库(CNKI)于1990年1月至2013年2月有关异体软骨组织移植物体外液体保存方法的研究，检索关键词分别为“Osteochondral al ograft； tissue culture；chondrocyte survival rate；in vitro”和“关节软骨；异体移植；液体保存”，排除发表时间较早或重复研究。结果与结论：目前主要有2种方法体外保存异体骨软骨。低温冷冻保存法保存后的软骨细胞存活率降低明显，影响移植效果，因此临床应用较少。液体保存法能够提高细胞成活率，保持组织活性，但保存时间不长，不能广泛应用。学者们又进一步改良液体培养环境以及培养液成分，延长了软骨组织体外保存时间，提高了软骨组织保存效果。
배경：유우관절연골무신경화혈관，기영양주요래원우활액화활막혈관적삼투작용，자신수복능력유한，인이여하경호적수복관절연골손상성위극대해결적의학난제。목적：회고근년래관우연골손상수복방법이급이체연골체외보존방법적문헌연구，조도괄합이체연골조직체외보존적최가보존조건이급배양액개질성분，종이제고이체연골조직체외보존효과。방법：계산궤검색PubMed수거고화중국기간전문수거고(CNKI)우1990년1월지2013년2월유관이체연골조직이식물체외액체보존방법적연구，검색관건사분별위“Osteochondral al ograft； tissue culture；chondrocyte survival rate；in vitro”화“관절연골；이체이식；액체보존”，배제발표시간교조혹중복연구。결과여결론：목전주요유2충방법체외보존이체골연골。저온냉동보존법보존후적연골세포존활솔강저명현，영향이식효과，인차림상응용교소。액체보존법능구제고세포성활솔，보지조직활성，단보존시간불장，불능엄범응용。학자문우진일보개량액체배양배경이급배양액성분，연장료연골조직체외보존시간，제고료연골조직보존효과。
BACKGROUND:Because of deficiency of nerves and blood vessels of articular cartilage, its nutrition is mainly derived from the synovial fluid or synovial vascular osmosis. Limited in their ability to repair itself, so how to repair articular cartilage damage better become medical problems to be solved. OBJECTIVE:To review to the literatures on the repair of cartilage damage method and al ogeneic cartilage in vitro preservation method in recent years, and to find the optimal preservation conditions and the culture medium composition that suitable for in vitro preservation of al ogeneic cartilage tissues, thus enhancing the effect of al ogenic cartilage in vitro preservation. METHODS:A computer-based online search was performed in the PubMed database and the CNKI database to search papers on the in vitro liquid preservation of al ogeneic cartilage graft published from January 1990 to February 2013. The key words were“osteochondral al ograft, tissue culture, chondrocyte survival rate, in vitro”in English and“articular cartilage;al ogeneic transplantation;liquid preservation”in Chinese. The articles published earlier and repetitive researches were excluded. RESULTS AND CONCLUSION:At present, there are two methods for the preservation of al ogenic osteochondral grafts. After cryopreservation, the survival rate of chondrocytes is decreased significantly which can affect the transplantation effect, therefore there is less clinical application. Liquid preservation method can enhance the survival rate of cells, maintain the organization activity, but the preservation time is short that cannot be widely used. Scholars have further improved the environment of liquid preservation and composition of liquid culture medium, extended the in vitro preservation time of cartilage tissue, and improved the effectiveness of cartilage tissue preservation.