中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
44期
7729-7732
,共4页
沈洪园%黄华扬%张余%郑小飞%李凭跃%徐国风%王泽锦%王庆
瀋洪園%黃華颺%張餘%鄭小飛%李憑躍%徐國風%王澤錦%王慶
침홍완%황화양%장여%정소비%리빙약%서국풍%왕택금%왕경
器官移植%组织移植%异种异体%同种异体%骨软骨移植%软骨损伤%修复%羊%猪%去抗原处理%省级基金
器官移植%組織移植%異種異體%同種異體%骨軟骨移植%軟骨損傷%脩複%羊%豬%去抗原處理%省級基金
기관이식%조직이식%이충이체%동충이체%골연골이식%연골손상%수복%양%저%거항원처리%성급기금
bone transplantation%cartilage,articular%transplantation,heterologous%goats%swine
背景:目前应用的各种软骨修复方法均有其优点和不足,修复组织多以纤维软骨为主,缺乏正常透明软骨的组织化学特性和生物力学特性。目的:探讨生物型异种骨软骨移植修复关节软骨损伤的可行性。方法:实验动物普通山羊随机分成两组,实验组供体为猪膝关节,以Smith & Nephew骨软骨移植器械,取直径4.5 mm、长10 mm的圆柱状骨软骨条,应用专利技术对动物骨软骨进行去抗原,对照组供体为羊膝关节骨软骨条,以低温冷冻法保存。分别于股骨滑车及内侧髁负重面的损伤处作骨软骨植入,术后16,32周处死动物,进行大体形态及病理切片观察。结果与结论:大体观察实验组部分软骨缺损移植处有纤维样的组织覆盖;部分移植物原表面软骨发黄,表面与周缘软骨有明显界限;对照组大体及切片镜下观察软骨缺损区基本被移植软骨覆盖,移植边缘可见裂隙。结果可见生物型异种骨软骨移植修复关节软骨损伤的作用与同种异体骨软骨比较有差别,同种异体骨软骨移植修复效果更佳。
揹景:目前應用的各種軟骨脩複方法均有其優點和不足,脩複組織多以纖維軟骨為主,缺乏正常透明軟骨的組織化學特性和生物力學特性。目的:探討生物型異種骨軟骨移植脩複關節軟骨損傷的可行性。方法:實驗動物普通山羊隨機分成兩組,實驗組供體為豬膝關節,以Smith & Nephew骨軟骨移植器械,取直徑4.5 mm、長10 mm的圓柱狀骨軟骨條,應用專利技術對動物骨軟骨進行去抗原,對照組供體為羊膝關節骨軟骨條,以低溫冷凍法保存。分彆于股骨滑車及內側髁負重麵的損傷處作骨軟骨植入,術後16,32週處死動物,進行大體形態及病理切片觀察。結果與結論:大體觀察實驗組部分軟骨缺損移植處有纖維樣的組織覆蓋;部分移植物原錶麵軟骨髮黃,錶麵與週緣軟骨有明顯界限;對照組大體及切片鏡下觀察軟骨缺損區基本被移植軟骨覆蓋,移植邊緣可見裂隙。結果可見生物型異種骨軟骨移植脩複關節軟骨損傷的作用與同種異體骨軟骨比較有差彆,同種異體骨軟骨移植脩複效果更佳。
배경:목전응용적각충연골수복방법균유기우점화불족,수복조직다이섬유연골위주,결핍정상투명연골적조직화학특성화생물역학특성。목적:탐토생물형이충골연골이식수복관절연골손상적가행성。방법:실험동물보통산양수궤분성량조,실험조공체위저슬관절,이Smith & Nephew골연골이식기계,취직경4.5 mm、장10 mm적원주상골연골조,응용전리기술대동물골연골진행거항원,대조조공체위양슬관절골연골조,이저온냉동법보존。분별우고골활차급내측과부중면적손상처작골연골식입,술후16,32주처사동물,진행대체형태급병리절편관찰。결과여결론:대체관찰실험조부분연골결손이식처유섬유양적조직복개;부분이식물원표면연골발황,표면여주연연골유명현계한;대조조대체급절편경하관찰연골결손구기본피이식연골복개,이식변연가견렬극。결과가견생물형이충골연골이식수복관절연골손상적작용여동충이체골연골비교유차별,동충이체골연골이식수복효과경가。
BACKGROUND:The methods used to repair articular cartilage defects currently have the cons and pros. Fibrocartilages are commonly used to repair tissues, and the fibrocartilage lacks of the tissue biomechanical properties and chemical properties of normal hyaline cartilage. OBJECTIVE:To investigate the feasibility of biological osteochondral xenogenic graft transplantation to repair articular cartilage defects. METHODS:The normal goats were randomly divided into two groups. The donor pig knee joints were the experimental group. Cylindrical osteochondral with the diameter of 4.5 mm and length of 10 mm were col ected with the Smith&Nephew osteochondral transplantation device, and the patented technology was used for deantigen. The donor goat knee joint osteochondrals were the control group and preserved with cryopreservation. The lesions on femoral trochlea and weight-bearing surface of medial condyle were selected respectively for osteochondral implantation, and the animals were sacrificed at 16 and 32 weeks after operation for the general and pathological section observation. RESULTS AND CONCLUSION:General observation in the experimental showed that the lesions were covered by fibroid tissue;some cartilage of the grafts turned yel ow and there was clear boundary between the surface and the peripheral cartilages;the general and section observation under microscope showed that lesions of the control group were covered by the grafts basical y, and cracks could be seen on the edge of the transplant part. The results show that there is difference between effects of biological osteochondral xenogenic graft transplantation and osteochondral al ograft transplantation for the repairing of articular cartilage defects, and osteochondral al ograft transplantation bas better effect.