CAJ | 학술논문

目的：检测基质金属蛋白酶(MMP )-9在胎盘组织中的表达及其与胎盘早剥的关系。方法选择2012年9月1日至2013年9月30日于青岛市中心医院经阴道分娩或剖宫产术后示胎盘早剥者60例为研究对象,并纳入胎盘早剥组。按照胎盘早剥的严重程度,将其分为不典型胎盘早剥组(n=30,Ⅰ度)和典型胎盘早剥组(n=30,Ⅱ度和Ⅲ度)。选择同期于本院经阴道分娩或剖宫产术后示正常胎盘者30例纳入对照组。3组产妇的年龄、体质量指数(BMI)、孕龄等比较,差异均无统计学意义(P>0.05)。采用免疫组化SP法检测胎盘组织中MMP-9的表达部位,蛋白免疫印迹法检测胎盘组织中MMP-9蛋白相对表达水平(本研究遵循的程序符合青岛市中心医院人体试验委员会制定的伦理学标准,得到该委员会批准,分组征得受试对象的同意,并与之签署临床研究知情同意书)。结果①胎盘早剥组和对照组胎盘组织中均有 MMP-9的表达,并主要表达于合体滋养细胞的细胞核及细胞质中。胎盘早剥组胎盘组织中 MMP-9阳性染色程度较对照组弱。②不典型胎盘早剥组和典型胎盘早剥组胎盘组织中 MMP-9蛋白相对表达水平分别为和0.68±0.05和0.34±0.03,对照组为0.94±0.06,3组间两两比较,差异均有统计学意义(P<0.01)。结论 MMP-9可能通过影响滋养细胞侵袭力而参与胎盘早剥的发病,从而成为临床预测胎盘早剥的指标。
목적：검측기질금속단백매(MMP )-9재태반조직중적표체급기여태반조박적관계。방법선택2012년9월1일지2013년9월30일우청도시중심의원경음도분면혹부궁산술후시태반조박자60례위연구대상,병납입태반조박조。안조태반조박적엄중정도,장기분위불전형태반조박조(n=30,Ⅰ도)화전형태반조박조(n=30,Ⅱ도화Ⅲ도)。선택동기우본원경음도분면혹부궁산술후시정상태반자30례납입대조조。3조산부적년령、체질량지수(BMI)、잉령등비교,차이균무통계학의의(P>0.05)。채용면역조화SP법검측태반조직중MMP-9적표체부위,단백면역인적법검측태반조직중MMP-9단백상대표체수평(본연구준순적정서부합청도시중심의원인체시험위원회제정적윤리학표준,득도해위원회비준,분조정득수시대상적동의,병여지첨서림상연구지정동의서)。결과①태반조박조화대조조태반조직중균유 MMP-9적표체,병주요표체우합체자양세포적세포핵급세포질중。태반조박조태반조직중 MMP-9양성염색정도교대조조약。②불전형태반조박조화전형태반조박조태반조직중 MMP-9단백상대표체수평분별위화0.68±0.05화0.34±0.03,대조조위0.94±0.06,3조간량량비교,차이균유통계학의의(P<0.01)。결론 MMP-9가능통과영향자양세포침습력이삼여태반조박적발병,종이성위림상예측태반조박적지표。
Objective To evaluate the expression of matrix metalloproteinase (MMP)-9 in placental tissues and the relationship between the expression of MMP-9 and placental abruption.Methods From September 1st,2012 to September 30th,2013,a total of 60 cases with placental abruption were included into this study (placental abruption group).They were divided into two groups according to the degrees of placentas,atypical placental abruption group (n=30)and typical placental abruption group (n=30).At the same time,another 30 cases with normal placental tissue were included into control group.There had no significant differences on age,body mass index (BMI)and gestational age among three groups (P>0.05). Placentas were collected and detected by immunohistochemical SP method to detect the localization of MMP-9 protein.Western blot analysis was used to detected MMP-9 protein levels.The study protocol was approved by the Ethical Review Board of Investigation of Qingdao Centre Medical Hospital.Informed consent was obtained from all participates.Results ①Expression of MMP-9 in placentas were found both in the placental abruption group and control group. MMP-9 positive immunostaining was apparently decreased in the placental abruption group when compared with control group.②MMP-9 protein levels significantly decreased in the atypical placental abruption group and typical placental abruption group (0.68±0.05,0.34±0.03)when compared with control group(0.94±0.06),with statistically significant differences (P<0.01).Conclusion MMP-9 might be a clinical prediction of placental abruption.