中国中西医结合外科杂志
中國中西醫結閤外科雜誌
중국중서의결합외과잡지
CHINESE JOURNAL OF SURGERY OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2014年
4期
390-392
,共3页
张淑坤%崔乃强%张艳敏%卓玉珍
張淑坤%崔迺彊%張豔敏%卓玉珍
장숙곤%최내강%장염민%탁옥진
清胰颗粒%重症急性胰腺炎%肺损伤%p38MAPK
清胰顆粒%重癥急性胰腺炎%肺損傷%p38MAPK
청이과립%중증급성이선염%폐손상%p38MAPK
Qingyi granules%acute hemorrhagic necrotizing pancreatitis,lung injury,p38 mitogen-activat-ed protein kinase
目的：探讨复方中药提取物清胰颗粒对急性出血坏死性胰腺炎（AHNP）大鼠早期肺损伤的影响和可能作用机制。方法：72只健康雄性Wistar大鼠随机分为假手术组、AHNP组、清胰颗粒组。采用胆胰管逆行注射3.5%牛磺胆酸钠建立AHNP动物模型。清胰颗粒组于制模前2 h给予清胰颗粒水溶液灌胃，以后每12 h灌胃1次。各组大鼠分别于制模后6 h、12 h和24 h取肺组织观察病理改变并进行病理评分，检测髓过氧化物酶（MPO）活性，并用荧光定量PCR检测Toll样受体4（TRL-4）和p38MAPK mRNA表达。结果：与同时点AHNP组比较，清胰颗粒组于造模后12 h和24 h肺组织病理评分、MPO活性降低（P＜0.05），于造模后6 h、12 h和24 h TRL-4和p38MAPK mRNA表达显著降低（P＜0.05）。结论：清胰颗粒能够减轻AHNP大鼠病理损伤和炎症反应，其作用机制可能与抑制p38MAPK信号通路有关。
目的：探討複方中藥提取物清胰顆粒對急性齣血壞死性胰腺炎（AHNP）大鼠早期肺損傷的影響和可能作用機製。方法：72隻健康雄性Wistar大鼠隨機分為假手術組、AHNP組、清胰顆粒組。採用膽胰管逆行註射3.5%牛磺膽痠鈉建立AHNP動物模型。清胰顆粒組于製模前2 h給予清胰顆粒水溶液灌胃，以後每12 h灌胃1次。各組大鼠分彆于製模後6 h、12 h和24 h取肺組織觀察病理改變併進行病理評分，檢測髓過氧化物酶（MPO）活性，併用熒光定量PCR檢測Toll樣受體4（TRL-4）和p38MAPK mRNA錶達。結果：與同時點AHNP組比較，清胰顆粒組于造模後12 h和24 h肺組織病理評分、MPO活性降低（P＜0.05），于造模後6 h、12 h和24 h TRL-4和p38MAPK mRNA錶達顯著降低（P＜0.05）。結論：清胰顆粒能夠減輕AHNP大鼠病理損傷和炎癥反應，其作用機製可能與抑製p38MAPK信號通路有關。
목적：탐토복방중약제취물청이과립대급성출혈배사성이선염（AHNP）대서조기폐손상적영향화가능작용궤제。방법：72지건강웅성Wistar대서수궤분위가수술조、AHNP조、청이과립조。채용담이관역행주사3.5%우광담산납건립AHNP동물모형。청이과립조우제모전2 h급여청이과립수용액관위，이후매12 h관위1차。각조대서분별우제모후6 h、12 h화24 h취폐조직관찰병리개변병진행병리평분，검측수과양화물매（MPO）활성，병용형광정량PCR검측Toll양수체4（TRL-4）화p38MAPK mRNA표체。결과：여동시점AHNP조비교，청이과립조우조모후12 h화24 h폐조직병리평분、MPO활성강저（P＜0.05），우조모후6 h、12 h화24 h TRL-4화p38MAPK mRNA표체현저강저（P＜0.05）。결론：청이과립능구감경AHNP대서병리손상화염증반응，기작용궤제가능여억제p38MAPK신호통로유관。
Objective To investigate the effect of Qingyi granules on early lung injury in rats with severe acute hemorrhagic necrotizing pancreatitis(AHNP). Methods Seventy-two healthy male Wistar rats were ran-domly divided into sham operation group, AHNP model group and AHNP +Qingyi granules (Qingyi)group. AHNP model was induced by retrograde injection of 3.5% sodium deoxycholate into the biliopancreatic duct in rats, the sham operation group were treated only with saline. Qingyi group were given Qingyi granules orally at 2 hours before induction of AHNP and Q12h after AHNP. At 6, 12, 24 h after AHNP, lung tissues were harvested for histopathological evaluation and for the determination of myeloperoxidase (MPO) activity, and the mRNA ex-pression of Toll-like receptor-4(TRL-4)and p38 mitogen-activated protein kinase (p38MAPK) was detected by using real-time PCR. Results Compared with AHNP model group, the histopathological score and the MPO activity of lung were reduced at 12, 24 h, the mRNA expression of TRL-4 and p38MAPK was all decreased at 6, 12, 24 h after AHNP in Qingyi group (P<0.05). Conclusion Qingyi granules can attenuate the early dam-age of lung in rats with AHNP maybe by inhibiting TRL-4 mediated p38MAPK signal pathway.
