中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2014年
4期
81-84
,共4页
非小细胞肺癌%蛋白磷酸酶2A的抑癌因子%实时定量%免疫组织化学
非小細胞肺癌%蛋白燐痠酶2A的抑癌因子%實時定量%免疫組織化學
비소세포폐암%단백린산매2A적억암인자%실시정량%면역조직화학
non-small cell lung cancer%cancerous inhibitor of protein phosphatase 2A%real-time%immunohistochemistry
目的：研究蛋白磷酸酶2A的抑癌因子（cancerous inhibitor of protein phosphatase 2A，CIP2A）在非小细胞肺癌（non-small cell lung cancer，NSCLC）中的差异表达情况及探讨其临床意义及潜在的预后价值。方法应用免疫组织化学和实时定量聚合酶链式反应（polymerase chain reaction，PCR）方法检测60例NSCLC癌组织及其癌旁正常组织2组中CIP2A表达情况，通过检测CIP2AmRNA及蛋白的表达情况，并分析其与临床病理资料和预后因素的关系。结果 CIP2A mRNA肺癌组织比癌旁组织高表达（P＜0.01）；CIP2A的蛋白在癌组织中的表达明显高于对应的癌旁组织（P＜0.01）。CIP2A的表达水平与非小细胞肺癌组织的分化程度及淋巴转移有关（P＜0.05），而与性别、年龄、组织学分级、肿瘤大小、TNM（tumor node metastasis）分期无关。结论 CIP2A在非小细胞肺癌组织中高表达，且随着非小细胞肺癌组织分化程度的增高及伴有淋巴结转移而增高。CIP2A及淋巴结转移情况是预后的独立危险因素。
目的：研究蛋白燐痠酶2A的抑癌因子（cancerous inhibitor of protein phosphatase 2A，CIP2A）在非小細胞肺癌（non-small cell lung cancer，NSCLC）中的差異錶達情況及探討其臨床意義及潛在的預後價值。方法應用免疫組織化學和實時定量聚閤酶鏈式反應（polymerase chain reaction，PCR）方法檢測60例NSCLC癌組織及其癌徬正常組織2組中CIP2A錶達情況，通過檢測CIP2AmRNA及蛋白的錶達情況，併分析其與臨床病理資料和預後因素的關繫。結果 CIP2A mRNA肺癌組織比癌徬組織高錶達（P＜0.01）；CIP2A的蛋白在癌組織中的錶達明顯高于對應的癌徬組織（P＜0.01）。CIP2A的錶達水平與非小細胞肺癌組織的分化程度及淋巴轉移有關（P＜0.05），而與性彆、年齡、組織學分級、腫瘤大小、TNM（tumor node metastasis）分期無關。結論 CIP2A在非小細胞肺癌組織中高錶達，且隨著非小細胞肺癌組織分化程度的增高及伴有淋巴結轉移而增高。CIP2A及淋巴結轉移情況是預後的獨立危險因素。
목적：연구단백린산매2A적억암인자（cancerous inhibitor of protein phosphatase 2A，CIP2A）재비소세포폐암（non-small cell lung cancer，NSCLC）중적차이표체정황급탐토기림상의의급잠재적예후개치。방법응용면역조직화학화실시정량취합매련식반응（polymerase chain reaction，PCR）방법검측60례NSCLC암조직급기암방정상조직2조중CIP2A표체정황，통과검측CIP2AmRNA급단백적표체정황，병분석기여림상병리자료화예후인소적관계。결과 CIP2A mRNA폐암조직비암방조직고표체（P＜0.01）；CIP2A적단백재암조직중적표체명현고우대응적암방조직（P＜0.01）。CIP2A적표체수평여비소세포폐암조직적분화정도급림파전이유관（P＜0.05），이여성별、년령、조직학분급、종류대소、TNM（tumor node metastasis）분기무관。결론 CIP2A재비소세포폐암조직중고표체，차수착비소세포폐암조직분화정도적증고급반유림파결전이이증고。CIP2A급림파결전이정황시예후적독립위험인소。
Objective To study the different expressions of cancerous inhibitor of protein phosphatase 2A (CIP2A)in non-small cell lung cancer (NSCLC),investigate its clinical significance and potential prognostic value.Methods Immunohistochemical and real-time polymerase chain reaction (PCR)were used to detect the expressions of CIP2A mRNA and protein in two groups of sixty paired NSCLC specimens and adjacent non-cancer tissues.The relationship between its expression and clinical pathological data and prognostic factors were analysed. Results The expression of CIP2A mRNA in NSCLC specimens was higher than those in adjacent non-cancer tissues(P<0.01 );The expression of CIP2A protein in NSCLC tissues was significantly higher than that in the corresponding adjacent non-cancer tissues (P<0.01 ).The expression of CIP2A was closely correlated with the differentiation and lymph node metastasis of NSCLC tissues (P<0.05 ),while incorrelated with sex,age,pathematology type,tumor size and tumor node metastasis (TNM)stage.Conclusion CIP2A highly expresses in NSCLC,and the expression of CIP2A will increase with the differentiation and lymph node metastasis of NSCLC tissues.The expression of CIP2A and lymph node metastasis can be used as an independent risk factor for the prognosis of patients with NSCLC.
