CAJ | 학술논문

目的：探讨茶多酚联合莱菔硫烷对肺癌组织中蛋白激酶A锚定蛋白95和细胞周期蛋白E2表达的影响。方法选取经病理证实并手术治疗的肺癌患者56例，随机分为联合给药组和对照组，每组28例。采用ELISA测定所有患者肺组织中蛋白激酶A锚定蛋白95及细胞周期蛋白E2的含量；免疫组化SP法测定组织中蛋白激酶A锚定蛋白95及细胞周期蛋白E2的表达并统计微血管密度（microvascular density，MVD）；Western Blot检测组织中蛋白激酶A锚定蛋白95及细胞周期蛋白E2蛋白表达情况。结果对照组肺癌患者组织中蛋白激酶A锚定蛋白95及细胞周期蛋白E2的含量均高于联合给药组，差异有统计学意义（P＜0.01）；免疫组化SP法结果显示肺癌患者癌变组织中蛋白激酶A锚定蛋白95及细胞周期蛋白E2的表达均呈阳性表达，且对照组织中MVD高于联合给药组（P＜0.01）；Western Blot结果显示对照组组织中蛋白激酶A锚定蛋白95及细胞周期蛋白E2蛋白表达高于联合给药组（P＜0.01）。结论蛋白激酶A锚定蛋白95及细胞周期蛋白E2在肺癌患者组织中均存在表达升高现象，2者表达呈正相关，与药物治疗呈负相关，可以作为肺癌的评估检测指标。
목적：탐토다다분연합래복류완대폐암조직중단백격매A묘정단백95화세포주기단백E2표체적영향。방법선취경병리증실병수술치료적폐암환자56례，수궤분위연합급약조화대조조，매조28례。채용ELISA측정소유환자폐조직중단백격매A묘정단백95급세포주기단백E2적함량；면역조화SP법측정조직중단백격매A묘정단백95급세포주기단백E2적표체병통계미혈관밀도（microvascular density，MVD）；Western Blot검측조직중단백격매A묘정단백95급세포주기단백E2단백표체정황。결과대조조폐암환자조직중단백격매A묘정단백95급세포주기단백E2적함량균고우연합급약조，차이유통계학의의（P＜0.01）；면역조화SP법결과현시폐암환자암변조직중단백격매A묘정단백95급세포주기단백E2적표체균정양성표체，차대조조직중MVD고우연합급약조（P＜0.01）；Western Blot결과현시대조조조직중단백격매A묘정단백95급세포주기단백E2단백표체고우연합급약조（P＜0.01）。결론단백격매A묘정단백95급세포주기단백E2재폐암환자조직중균존재표체승고현상，2자표체정정상관，여약물치료정부상관，가이작위폐암적평고검측지표。
Objective To investigate the effect of tea polyphenols and sulforaphane on the expression of protein kinase A anchoring protein 95 and cyclin E2 in lung cancer tissue.Methods 56 cases confirmed by pathology and operation therapy for lung cancer patients,were randomly divided into combined treatment group and control group,each had 28 cases.The content of protein kinase A anchoring protein 95 and cell cycle protein E2 in all patients were determined with ELISA.The expression of protein kinase A anchoring protein 95,cyclin E2 and statistics of microvessel density(MVD) were detected by immunohistochemical SP method.The protein levels of protein kinase A anchoring protein 95 and cell cycle protein E2 protein were detected by Western Blot. Results The contents of protein kinase A anchoring protein 95 and cyclin E2 in control group were higher than that in combination group,the differences were statistically significant(P<0.01 ).Immunohistochemical SP results showed that protein kinase A anchoring protein 95 and cyclin E2 expression all had positive expression,and the MVD in control group was higher than combination group(P<0.01);Western Blot results showed that the expressions of protein kinase A anchoring protein 95 and cyclin E2 protein in control group were higher than combination group(P<0.01).Conclusion Protein kinase A anchoring protein 95 and cyclin E2 expressions are increased in both groups in lung cancer tissues,and they are positively correlated,negatively correlated with drug treatment,which can be used as the indexes of lung cancer evaluation.