CAJ | 학술논문

目的：探讨中药甘遂所致小鼠肝脏氧化损伤的作用机制。方法昆明种小鼠40只，雌雄各半，随机分为4组，分别为4 g/kg组（1/8 LD50）、2 g/kg组（1/16 LD50）、1 g/kg组（1/32 LD50）及正常对照组，连续灌胃给药21 d后测定小鼠体质量及肝脏脏器系数；HE染色观察各组小鼠肝组织形态学变化；紫外分光光度法测定各组小鼠血清谷丙转氨酶（alanine amiotransferase，ALT）、谷草转氨酶（aspartate aminotransferase，AST）酶活性；肝组织超氧化物歧化酶（superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（glutathione peroxidases，GSH-Px）水平，丙二醛（malondialdehyde，MDA）、超氧阴离子自由基（O2-·）、羟自由基（OH·）含量的变化，流式细胞仪检测肝细胞内活性氧（reactive oxygen species，ROS）的变化。结果随着甘遂剂量增加，小鼠体质量明显下降，肝脏脏器系数显著升高（P＜0.05）；血清ALT 和 AST 活性显著升高（P ＜0.05）；肝组织 SOD、GSH-PX 活性显著降低（P ＜0.05）、MDA 含量显著升高（P＜0.05）；肝组织氧自由基O2-·、OH·、ROS含量显著增高（P＜0.05）。结论甘遂通过脂质过氧化作用而引起肝损伤。
목적：탐토중약감수소치소서간장양화손상적작용궤제。방법곤명충소서40지，자웅각반，수궤분위4조，분별위4 g/kg조（1/8 LD50）、2 g/kg조（1/16 LD50）、1 g/kg조（1/32 LD50）급정상대조조，련속관위급약21 d후측정소서체질량급간장장기계수；HE염색관찰각조소서간조직형태학변화；자외분광광도법측정각조소서혈청곡병전안매（alanine amiotransferase，ALT）、곡초전안매（aspartate aminotransferase，AST）매활성；간조직초양화물기화매（superoxide dismutase，SOD）、곡광감태과양화물매（glutathione peroxidases，GSH-Px）수평，병이철（malondialdehyde，MDA）、초양음리자자유기（O2-·）、간자유기（OH·）함량적변화，류식세포의검측간세포내활성양（reactive oxygen species，ROS）적변화。결과수착감수제량증가，소서체질량명현하강，간장장기계수현저승고（P＜0.05）；혈청ALT 화 AST 활성현저승고（P ＜0.05）；간조직 SOD、GSH-PX 활성현저강저（P ＜0.05）、MDA 함량현저승고（P＜0.05）；간조직양자유기O2-·、OH·、ROS함량현저증고（P＜0.05）。결론감수통과지질과양화작용이인기간손상。
Objective To study the mechanisms of Chinese medicine Euphorbia kansui induced liver damage. Methods 40 mices were selected and radomly divided into four groups according to the dose of Chinese medicine Euphorbia kansui used,there were 4 g/kg group(1/8 LD50 ),2 g/kg group(1/16 LD50),1 g/kg group(1/32 LD)50,and control group,which were given saline. After continuous administration for 21 d by ig,the body weight and liver coefficient of mice in all groups were determined,liver histological changes were detected by HE staining. The serum enzymatic activity of alanine aminotransferase (ALT)and aspartate aminotransferase (AST),the levels of superoxide dismutase (SOD)and glutathione peroxidase (GSH-Px),the content of malondialdehyde (MDA),superoxide anion radical (O2-·)and hydroxyl radical (OH·)were all measured by ultraviolet spectrophotometry. The changes of reactive oxygen species (ROS)content were detected by flow cytometry. Results With the increase dose of Chinese medicine Euphorbia kansui,the body weight of rats were decreased,and liver coefficient were increased(P<0.05). The serum ALT,AST activity were significantly increased,SOD and GSH-Px activity were significantly decreased(P<0.05),MDA content were increased significantly,radical O2-·, OH·and intracellular ROS levels were all increased (P<0.05 ). Conclusion Chinese medicine Euphorbia kansui induced liver injury though lipid peroxidation.