中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2014年
4期
47-49,52
,共4页
虫草菌液%糖尿病肾病%肾小管上皮细胞
蟲草菌液%糖尿病腎病%腎小管上皮細胞
충초균액%당뇨병신병%신소관상피세포
Cordyceps sinensis liquid%diabetic nephropathy%renal tubular epithelial cell
目的：探讨虫草菌液对糖尿病肾病肾小管上皮细胞转分化的影响。方法将不同浓度的虫草菌液和高糖作用于人近端肾小管上皮细胞，根据加入剂量和组份不同分成以下5组：对照组给予5.5 mmol/L D-葡萄糖；高糖组给予30 mmol/L D-葡萄糖；实验组1给予30 mmol/L D-葡萄糖＋5μg/mL 虫草菌液；实验组2给予30 mmol/L D-葡萄糖＋10μg/mL 虫草菌液；实验组3给予30 mmol/L D-葡萄糖＋20μg/mL虫草菌液。检测各组细胞内E-钙粘蛋白（E-Cadherin），纤连蛋白（fibrous protein，FN），α-平滑肌肌动蛋白（a-smooth muscle actin，α-SMA）及整合素连接激酶（Integrin Linked Kinase，ILK）蛋白的表达。结果与对照组相比，高糖组E-cadherin蛋白表达显著降低（P＜0.01），且随着虫草菌液加入的量升高，E-cadherin蛋白表达也逐渐升高（均P＜0.01）。与对照组相比，高糖组α-SMA蛋白和FN蛋白表达均显著升高（P＜0.01），且2蛋白在实验组2和实验组3中表达均较高糖组显著下降（均P＜0.01）。与对照组相比，高糖组ILK蛋白表达显著升高（P＜0.01），实验组3 ILK蛋白表达较高糖组显著下降（P＜0.01）。结论虫草菌液能够抑制肾小管上皮细胞向肌成纤维细胞的转化，从而抑制肾小管间质纤维化。
目的：探討蟲草菌液對糖尿病腎病腎小管上皮細胞轉分化的影響。方法將不同濃度的蟲草菌液和高糖作用于人近耑腎小管上皮細胞，根據加入劑量和組份不同分成以下5組：對照組給予5.5 mmol/L D-葡萄糖；高糖組給予30 mmol/L D-葡萄糖；實驗組1給予30 mmol/L D-葡萄糖＋5μg/mL 蟲草菌液；實驗組2給予30 mmol/L D-葡萄糖＋10μg/mL 蟲草菌液；實驗組3給予30 mmol/L D-葡萄糖＋20μg/mL蟲草菌液。檢測各組細胞內E-鈣粘蛋白（E-Cadherin），纖連蛋白（fibrous protein，FN），α-平滑肌肌動蛋白（a-smooth muscle actin，α-SMA）及整閤素連接激酶（Integrin Linked Kinase，ILK）蛋白的錶達。結果與對照組相比，高糖組E-cadherin蛋白錶達顯著降低（P＜0.01），且隨著蟲草菌液加入的量升高，E-cadherin蛋白錶達也逐漸升高（均P＜0.01）。與對照組相比，高糖組α-SMA蛋白和FN蛋白錶達均顯著升高（P＜0.01），且2蛋白在實驗組2和實驗組3中錶達均較高糖組顯著下降（均P＜0.01）。與對照組相比，高糖組ILK蛋白錶達顯著升高（P＜0.01），實驗組3 ILK蛋白錶達較高糖組顯著下降（P＜0.01）。結論蟲草菌液能夠抑製腎小管上皮細胞嚮肌成纖維細胞的轉化，從而抑製腎小管間質纖維化。
목적：탐토충초균액대당뇨병신병신소관상피세포전분화적영향。방법장불동농도적충초균액화고당작용우인근단신소관상피세포，근거가입제량화조빈불동분성이하5조：대조조급여5.5 mmol/L D-포도당；고당조급여30 mmol/L D-포도당；실험조1급여30 mmol/L D-포도당＋5μg/mL 충초균액；실험조2급여30 mmol/L D-포도당＋10μg/mL 충초균액；실험조3급여30 mmol/L D-포도당＋20μg/mL충초균액。검측각조세포내E-개점단백（E-Cadherin），섬련단백（fibrous protein，FN），α-평활기기동단백（a-smooth muscle actin，α-SMA）급정합소련접격매（Integrin Linked Kinase，ILK）단백적표체。결과여대조조상비，고당조E-cadherin단백표체현저강저（P＜0.01），차수착충초균액가입적량승고，E-cadherin단백표체야축점승고（균P＜0.01）。여대조조상비，고당조α-SMA단백화FN단백표체균현저승고（P＜0.01），차2단백재실험조2화실험조3중표체균교고당조현저하강（균P＜0.01）。여대조조상비，고당조ILK단백표체현저승고（P＜0.01），실험조3 ILK단백표체교고당조현저하강（P＜0.01）。결론충초균액능구억제신소관상피세포향기성섬유세포적전화，종이억제신소관간질섬유화。
Objective To discuss the effect of Cordyceps sinensis liquid on Diabetic Nephropathy renal tubular epithelial cell transdifferentiation.Methods Diabetic Nephropathy renal tubular epithelial cells were treated with different dose of Cordyceps sinensis liquid and high glucose,and divided into five groups.Control group was added 5.5 mmol/L D-glucose,High glucose group was added 30 mmol/L D-glucose, Experimental group 1 was added 30 mmol/L D-glucose and 5μg/mL Cordyceps sinensis liquid,Experimental group 2 was added 30 mmol/L D-glucose and 10 μg/mL Cordyceps sinensis liquid,Experimental group 3 was added 30 mmol/L D-glucose and 20 μg/mL Cordyceps sinensis liquid.The expression of E-cadherin protein,FN protein,α-SMA protein and ILK protein in each group were detected by western blot. Results Compared with control group,the expression of E-cadherin protein in high glucose was decreased significantly(P<0.01),and with the addition of Cordyceps bacteria increases,E-cadherin protein expression in three Experiment group were gradually increased(P<0.01).Compared with control group,the expression ofα-SMA protein and FN protein in High glucose group were increased significantly (P <0.01 ),and both two protein in Experiment group 2 and Experiment 3 were significantly lower than High glucose group(P<0.01).Compared with control group,the expression of ILK protein in high glucose group was significantly higher(P<0.01 ),and its expression in Experiment group 3 was significantly lower than High glucose group (P<0.01 ). Conclusion Cordyceps sinensis liquid can inhibit diabetic nephropathy renal tubular epithelial cell translating into myofibroblast,then inhibit renal tubule interstitial fibrosis.
