CAJ | 학술논문

目的：探讨血清脂肪酸结合蛋白（intestinal fatty acid binding protein，I-FABP）在脓毒症鼠肠功能障碍评估中的作用及谷氨酰胺对其表达水平的影响。方法以健康SD大鼠腹腔注射大肠杆菌内毒素脂多糖（lipopolysaccharide，LPS）建立脓毒症模型，随机分为3组，对照组不建模，仅给予百普素喂养，模型组建模后给予百普素喂养，谷氨酰胺组建模后同时给予百普素和谷氨酰胺喂养。评估血清I-FABP浓度与肠粘膜损伤指数的相关性及比较3组大鼠血清I-FABP含量。结果血清I-FABP浓度与肠粘膜损伤指数呈正相关（P＜0.05）；与对照组相比，模型组和谷氨酰胺组血清I-FABP浓度均明显增高（P＜0.05）；而谷氨酰胺组中血清I-FABP浓度显著低于模型组（P＜0.05）。结论血清I-FABP可作为脓毒症肠功能障碍评估的无创性指标；谷氨酰胺能改善脓毒症鼠的肠粘膜的损伤程度。
목적：탐토혈청지방산결합단백（intestinal fatty acid binding protein，I-FABP）재농독증서장공능장애평고중적작용급곡안선알대기표체수평적영향。방법이건강SD대서복강주사대장간균내독소지다당（lipopolysaccharide，LPS）건립농독증모형，수궤분위3조，대조조불건모，부급여백보소위양，모형조건모후급여백보소위양，곡안선알조건모후동시급여백보소화곡안선알위양。평고혈청I-FABP농도여장점막손상지수적상관성급비교3조대서혈청I-FABP함량。결과혈청I-FABP농도여장점막손상지수정정상관（P＜0.05）；여대조조상비，모형조화곡안선알조혈청I-FABP농도균명현증고（P＜0.05）；이곡안선알조중혈청I-FABP농도현저저우모형조（P＜0.05）。결론혈청I-FABP가작위농독증장공능장애평고적무창성지표；곡안선알능개선농독증서적장점막적손상정도。
Objective To investigate the role of intestinal fatty acid binding protein(I-FABP)in evaluating intestinal dysfunction of septic rats and the effect of glutamine on I-FABP expression.Methods Rats were divided into 3 groups,control group were only fed with Peptisorb,model group were fed with Peptisorb after intraperitoneal injection with E.coli endotoxin lipopolysaccharidegiven and glutamine group were added glutamine on basis of model group.The correlation between serum I-FABP level and intestinal mucosa damage index were analyzed and the concentrations of serum I-FABP in each group were observed and compared. Results The serum level of I-FABP in rats were correlated with the Chiu’s score of intestinal mucosa,mucosa thickness and villus length(P<0.05 ).Compared with control group,the concentration of serum I-FABP in model group and glutamine group were significantly increased(P<0.05),but which in glutamine group was lower than that in model group(P<0.05).Conclusions Serum I-FABP could be an non-invasive diagnosis index for evaluating acute intestinal dysfunction in septic rats.In addition,dietary glutamine supplementation may ameliorate sepsis-induced intestinal epithelial injury in rats.