CAJ | 학술논문

目的：通过检测大骨节病儿童软骨细胞中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）的表达，探讨细胞因子与大骨节病（KBD）之间的关系。方法5例KBD儿童（KBD组）手指软骨组织来自西安交通大学医学院地方病研究所保存的大骨节病患儿尸检样本；5例正常儿童（对照组）手指软骨组织来自陕西省西安市非KBD病区，其中3例来自车祸死亡儿童，2例为先天6指畸形手术标本。采用免疫组织化学染色的方法，分别检测软骨中IL-1β、IL-6、TNF-α的表达；并将关节软骨细胞分层（表层、中层、深层），分析软骨各层中IL-1β、IL-6、TNF-α的表达。结果 KBD组关节软骨表层、中层、深层IL-1β阳性软骨细胞密度（63.50±7.19、54.75±5.50、66.20±9.91）均高于对照组（5.75±1.26、0.00±0.00、0.00±0.00，P均<0.05）；KBD组关节软骨表层IL-6阳性软骨细胞密度（55.25±6.24）高于对照组（0.00±0.00，P<0.05）；KBD组关节软骨表层、中层、深层TNF-α阳性软骨细胞密度（33.25±6.50、3.75±0.96、29.80±1.92）均高于对照组（3.74±0.82、0.00±0.00、0.00±0.00，P均<0.05）。结论 KBD儿童软骨细胞中IL-1β，IL-6和TNF-α表达显著升高，细胞因子的过量表达可能参与KBD软骨细胞死亡与软骨破坏过程。
목적：통과검측대골절병인동연골세포중백세포개소-1β（IL-1β）、백세포개소-6（IL-6）、종류배사인자-α（TNF-α）적표체，탐토세포인자여대골절병（KBD）지간적관계。방법5례KBD인동（KBD조）수지연골조직래자서안교통대학의학원지방병연구소보존적대골절병환인시검양본；5례정상인동（대조조）수지연골조직래자합서성서안시비KBD병구，기중3례래자차화사망인동，2례위선천6지기형수술표본。채용면역조직화학염색적방법，분별검측연골중IL-1β、IL-6、TNF-α적표체；병장관절연골세포분층（표층、중층、심층），분석연골각층중IL-1β、IL-6、TNF-α적표체。결과 KBD조관절연골표층、중층、심층IL-1β양성연골세포밀도（63.50±7.19、54.75±5.50、66.20±9.91）균고우대조조（5.75±1.26、0.00±0.00、0.00±0.00，P균<0.05）；KBD조관절연골표층IL-6양성연골세포밀도（55.25±6.24）고우대조조（0.00±0.00，P<0.05）；KBD조관절연골표층、중층、심층TNF-α양성연골세포밀도（33.25±6.50、3.75±0.96、29.80±1.92）균고우대조조（3.74±0.82、0.00±0.00、0.00±0.00，P균<0.05）。결론 KBD인동연골세포중IL-1β，IL-6화TNF-α표체현저승고，세포인자적과량표체가능삼여KBD연골세포사망여연골파배과정。
Objective To investigate the expressions of interleukin-1β(IL-1β), interleukin-6(IL-6) and tumor necrosis factor alpha(TNF-α) in cartilage of children with Kashin-Beck disease(KBD) in order to provide a possible mechanism of the disease. Methods Articular cartilage tissues of 5 KBD children(KBD group) were selected from KBD children autopsy samples keeping in Institute of Endemic Diseases, Medical School of Xi’an Jiaotong University; articular cartilage tissues of 5 normal children ( control group ) were selected from non-KBD areas of Shaanxi Province, three cases were from accident death children, two cases were the samples of congential malformation of six finger. Expressions of IL-1β, IL-6 and TNF-α in the cartilage were detected using immunohistochemistry; the cells of articular cartilage were divided into three areas (superficial zone, middle zone and deep zone) to analyze the expressions of IL-1β, IL-6 and TNF-α. Results The expressions of IL-1β in superficial zone , middle zone and deep zone of articular cartilage of KBD group (63.50 ± 7.19, 54.75 ± 5.50, 66.20 ± 9.91) were significantly higher than those of control group(5.75 ± 1.26, 0.00 ± 0.00, 0.00 ± 0.00, all P<0.05). The expression of IL-6 in superficial zone of articular cartilage in KBD group(55.25 ± 6.24) was significantly higher than that of control group(0.00 ± 0.00, P<0.05). The expressions of TNF-αin all zone of articular cartilage of KBD group(33.25 ± 6.50, 3.75 ± 0.96, 29.80 ± 1.92) were significantly higher than those of control group (3.74 ± 0.82, 0.00 ± 0.00, 0.00 ± 0.00, all P < 0.05). Conclusion The levels of IL-1β, IL-6 and TNF-α are up-regulated in articular cartilage of KBD children, suggesting that cytokines may play an important role in matrix degradation in KBD children cartilage.