CAJ | 학술논문

目的观察血管内皮生长因子受体3（VEGFR-3）及肾小球足突细胞膜黏蛋白（podoplanin）在结直肠癌组织中的表达，探讨VEGFR-3及podoplanin在结直肠癌淋巴管生成及转移中的作用。方法应用免疫组织化学法检测52例结直肠癌组织、20例大肠息肉组织和20例正常对照组织中VEGFR-3及podoplanin的表达，分析其与结直肠癌患者的临床病理参数的相关性。结果 VEGFR-3主要表达在结直肠癌细胞胞膜及胞质中，阳性表达率67.30％，明显高于在大肠息肉组织和正常大肠组织中的阳性表达率（χ2分别=6.75、16.77，P均＜0.05）；其在小淋巴管内皮的阳性表达率仅为9.61％。 podoplanin主要表达在癌细胞淋巴管内皮细胞上， podoplanin标记的淋巴管密度（LMVD）明显高于大肠息肉组织和正常大肠组织（t分别=3.15、3.54，P均＜0.05）。 VEGFR-3、podo planin标记的LMVD的表达与Dukes分期、淋巴管转移有关（χ2分别=12.63、13.26，t分别=3.57、3.02，P均＜0.05）。结论 VEGFR-3很少在癌细胞淋巴管内皮表达，VEGFR-3作为淋巴管标记物的依据不足；podoplanin在结直肠癌淋巴管内皮细胞高度表达，可以作为结直肠癌淋巴管标记物。
목적관찰혈관내피생장인자수체3（VEGFR-3）급신소구족돌세포막점단백（podoplanin）재결직장암조직중적표체，탐토VEGFR-3급podoplanin재결직장암림파관생성급전이중적작용。방법응용면역조직화학법검측52례결직장암조직、20례대장식육조직화20례정상대조조직중VEGFR-3급podoplanin적표체，분석기여결직장암환자적림상병리삼수적상관성。결과 VEGFR-3주요표체재결직장암세포포막급포질중，양성표체솔67.30％，명현고우재대장식육조직화정상대장조직중적양성표체솔（χ2분별=6.75、16.77，P균＜0.05）；기재소림파관내피적양성표체솔부위9.61％。 podoplanin주요표체재암세포림파관내피세포상， podoplanin표기적림파관밀도（LMVD）명현고우대장식육조직화정상대장조직（t분별=3.15、3.54，P균＜0.05）。 VEGFR-3、podo planin표기적LMVD적표체여Dukes분기、림파관전이유관（χ2분별=12.63、13.26，t분별=3.57、3.02，P균＜0.05）。결론 VEGFR-3흔소재암세포림파관내피표체，VEGFR-3작위림파관표기물적의거불족；podoplanin재결직장암림파관내피세포고도표체，가이작위결직장암림파관표기물。
Objective To observe the expression of VEGFR-3 and podoplanin in colorectal carcinoma and investigate the role of vascular endothelial growth factor receptor 3 (VEGFR-3)and podoplanin in lymphangiogenesis and lymphatic metastasis of colorectal carcinoma. Methods The expression of VEGFR-3 and podoplanin in specimens of 52 colorectal carcinoma, 20 colorectal polyps tissues and 20 colorectal normal tissues were detected by immunohistochemistry. And the relationships between VEGFR-3, podoplanin and clinicopathological features of colorectal carcinoma were analyzed. Results VEGFR-3 was mainly expressed in colorectal carcinoma cell membrane and cytoplasm, the positive rate were 67.30% which were significantly higher than those of the colorectal polyps tissues and the normal bowel tissues (χ2=6.75,16.77,P<0.05). It was marginally expressed in the small lymphatic endothelium, the positive rate was 9.61%. podoplanin was mainly expressed in lymphatic endothelial cells. The lymphatic vessel density (LMVD) marked by podoplanin of colorectal carcinoma were significantly higher than those of the colorectal polyps tissues and the normal bow-el tissues (t=3.15,3.54,P<0.05). The expression of VEGFR-3 and podoplanin in colorectal carcinoma was significantly related with Dukes'stage and lymph node metastasis(χ2=12.63,13.26;t=3.57,3.02;P<0.05). Conclusion VEGFR-3 was rarely expressed in colorectal cancer lymphatic endothelium. VEGFR-3 as the basis of lymphatic marker has not adequate proofs. podoplanin that express high specificity in colorectal carcinoma can be used to mark lymphatic endothelial cells.