CAJ | 학술논문

目的：观察NOD2基因对舌鳞癌Tca8113细胞增殖、凋亡的影响。方法：构建NOD2表达载体（NOD2-pEZ-M29）和NOD2-shRNA表达载体，分别转染舌鳞癌Tca8113细胞48 h后，RT-PCR和Western blot 法检测NOD2和HBD-2分子及蛋白表达，MTT法检测细胞增殖，流式细胞仪检测细胞凋亡比例。结果：转染NOD2表达载体的Tca8113细胞NOD2和HBD-2表达较对照组显著增高，细胞增殖速度较对照组慢，凋亡较对照组高；转染NOD2-shRNA载体的Tca8113细胞NOD2和HBD-2表达较对照组显著降低，细胞增殖速度较对照组快，凋亡较对照组低。结论：NOD2可促进舌鳞癌细胞凋亡，抑制其增殖。在舌鳞癌细胞中，NOD2与HBD-2的表达呈正相关。
목적：관찰NOD2기인대설린암Tca8113세포증식、조망적영향。방법：구건NOD2표체재체（NOD2-pEZ-M29）화NOD2-shRNA표체재체，분별전염설린암Tca8113세포48 h후，RT-PCR화Western blot 법검측NOD2화HBD-2분자급단백표체，MTT법검측세포증식，류식세포의검측세포조망비례。결과：전염NOD2표체재체적Tca8113세포NOD2화HBD-2표체교대조조현저증고，세포증식속도교대조조만，조망교대조조고；전염NOD2-shRNA재체적Tca8113세포NOD2화HBD-2표체교대조조현저강저，세포증식속도교대조조쾌，조망교대조조저。결론：NOD2가촉진설린암세포조망，억제기증식。재설린암세포중，NOD2여HBD-2적표체정정상관。
Objective:To explore the effects of NOD2 gene on the proliferation and apoptosis of tongue squamous cell carcinoma Tca8113 cells.Methods:NOD2 expression vector(NOD2-pEZ-M29)and NOD2-shRNA vector were established,then were trans-fected into Tca8113 cells respectively.Expressions of HBD-2 and NOD2 in the cells was detected by RT-PCR and Western blot.Cell proliferation was examined by MTT assay and apoptosis by flow cytometry at 48h post transfection.Results:Compared with the control group,the expression of NOD2 and HBD-2 in NOD2-pEZ-M29 transfection group was significantly higher and markedly lower in NOD2-shRNA group.The proliferation rate of Tca8113 cells was markedly lower in NOD2-pEZ-M29 transfection group and signifi-cantly higher in NOD2-shRNA group while the apoptosis rate was significantly higher in NOD2-pEZ-M29 transfection group and sig-nificantly lower in NOD2-shRNA group.Conclusion:In Tca8113 cells NOD2 expression was positively correlated with HBD-2 ex-pression.NOD2 gene may promote the apoptosis,inhibit the proliferation of Tca8113 cells.