CAJ | 학술논문

目的：观察类胰蛋白酶（tryptase）和T细胞免疫球蛋白粘蛋白-3（TIM-3）在慢性牙周炎牙龈组织肥大细胞中的表达。方法：纳入正常对照受试者25例，轻度牙周炎患者28例，重度牙周炎患者30例。牙龈组织标本经HE染色，光学显微镜下观察；免疫荧光双染色，荧光显微镜下观察。结果：牙龈组织中的炎症反应程度与慢性牙周炎的病变程度趋势一致。牙周炎组牙龈组织tryptase和TIM-3双阳性肥大细胞数量均显著升高（P＜0．05）；重度牙周炎组牙龈组织中的双阳性肥大细胞数量显著高于轻度牙周炎组（P＜0．05）。结论：慢性牙周炎牙龈组织中的tryptase和TIM-3双阳性肥大细胞数量与牙周炎病变程度的趋势相一致，提示tryptase和TIM-3双阳性肥大细胞可能参与慢性牙周炎的免疫调节。
목적：관찰류이단백매（tryptase）화T세포면역구단백점단백-3（TIM-3）재만성아주염아간조직비대세포중적표체。방법：납입정상대조수시자25례，경도아주염환자28례，중도아주염환자30례。아간조직표본경HE염색，광학현미경하관찰；면역형광쌍염색，형광현미경하관찰。결과：아간조직중적염증반응정도여만성아주염적병변정도추세일치。아주염조아간조직tryptase화TIM-3쌍양성비대세포수량균현저승고（P＜0．05）；중도아주염조아간조직중적쌍양성비대세포수량현저고우경도아주염조（P＜0．05）。결론：만성아주염아간조직중적tryptase화TIM-3쌍양성비대세포수량여아주염병변정도적추세상일치，제시tryptase화TIM-3쌍양성비대세포가능삼여만성아주염적면역조절。
Objective:To quantify the tryptase and T cell immunoglobulin mucin domain-3(TIM-3)double positive mast cells in hu-man chronic periodontitis tissue using double immunofluorescence staining.Methods:25 healthy controls,28 chronic mild periodontitis and 30 chronic advanced periodontitis patients were included.The gingival specimens were stained with HE for histology,and with double immunofluorescence staining for the identification of tryptase and TIM-3 double positive mast cells in gingival tissue.Results:In chronic periodontitis tissue the degree of gingival inflammation was significantly increased,the densities(cells/mm2 )of tryptase and TIM-3 double positive mast cells were significantly increased(P<0.05),in addition,that in chronic advanced periodontitis group was significantly higher than in the chronic mild periodontitis group(P<0.05).Conclusion:Tryptase and TIM-3 double positive mast cells has the similar tendency as the severity of periodontitis inflammation in human periodontitis tissue.Tryptase and TIM-3 double positive mast cells may play an important role in human chronic periodontitis.