CAJ | 학술논문

目的：研究牙周炎对Ⅱ型糖尿病大鼠胰岛细胞凋亡相关蛋白表达的影响。方法：自发Ⅱ型糖尿病OLETF大鼠按照是否复合牙周炎模型随机分为复合组（合并牙周炎）和糖尿病组（牙周健康）；同种系同周龄糖耐量正常LETO大鼠随机分为牙周炎组和正常组。复合组和牙周炎组采取丝线结扎联合涂菌建立慢性牙周炎模型，建模20周后处死大鼠，取胰腺组织脱水包埋制作切片。免疫组化半定量分析胰岛组织Bax、Bcl-2和Caspase-3蛋白表达量。结果：正常组和牙周炎组大鼠胰岛细胞Bax、Bcl-2和Caspase-3蛋白表达差异均无显著性（P＝0．324，P＝0．091，P＝0．852）；和糖尿病组相比复合组 Bax、Caspase-3蛋白表达显著上升（P＝0．000，P＝0．000），Bcl-2蛋白表达显著下降（P＝0．022）。结论：牙周炎对无糖尿病大鼠胰岛细胞凋亡相关蛋白的表达没有影响，但在糖尿病状态下，牙周炎可影响凋亡相关蛋白的表达。
목적：연구아주염대Ⅱ형당뇨병대서이도세포조망상관단백표체적영향。방법：자발Ⅱ형당뇨병OLETF대서안조시부복합아주염모형수궤분위복합조（합병아주염）화당뇨병조（아주건강）；동충계동주령당내량정상LETO대서수궤분위아주염조화정상조。복합조화아주염조채취사선결찰연합도균건립만성아주염모형，건모20주후처사대서，취이선조직탈수포매제작절편。면역조화반정량분석이도조직Bax、Bcl-2화Caspase-3단백표체량。결과：정상조화아주염조대서이도세포Bax、Bcl-2화Caspase-3단백표체차이균무현저성（P＝0．324，P＝0．091，P＝0．852）；화당뇨병조상비복합조 Bax、Caspase-3단백표체현저상승（P＝0．000，P＝0．000），Bcl-2단백표체현저하강（P＝0．022）。결론：아주염대무당뇨병대서이도세포조망상관단백적표체몰유영향，단재당뇨병상태하，아주염가영향조망상관단백적표체。
Objective:To evaluate the effects of periodontitis on the expression of apoptosis-related proteins in pancreas of rats with Type 2 Diabetes Mellitus.Methods:Spontaneously type 2 diabetic OLETF rats were randomly divided into 2 groups:diabetes with or without periodontitis(diabetes group and combination group).LETO rats with the same germline and the same age but having normal glucose tolerance were randomly divided into control group and periodontitis group.20 weeks after periodontitis were established,all the rats were sacrificed and the pancreas were pathologically examined by HE staining.The expression of Bax,Bcl-2 and Caspase-3 in the pancreas islet were detected by immunohistochemistry staining and semi-quantitative analysis.Results:The expression of Bax, Bcl-2 and Caspase-3 in the pancreas islet was no significant difference between control and periodontitis groups(P=0.324,P=0.091,P=0.852).Compared with diabetes group,the expression of Bax and Caspase-3 in combination group showed a significant increase(P=0.000,P=0.000),and the expression of Bcl-2 was significantly decreased(P=0.022).Conclusion:Under healthy conditions,periodontitis has no effect on the expression of apoptosis-related proteins in rat pancreas islet.However,in rats with diabe-tes,periodontitis may affect the expression of apoptosis-related proteins in pancreas islet.