齐鲁师范学院学报
齊魯師範學院學報
제로사범학원학보
JOURNAL OF SHANDONG EDUCATION INSTITUTE
2014年
4期
124-127
,共4页
辣椒素%HPLC法%乳膏%含量测定
辣椒素%HPLC法%乳膏%含量測定
랄초소%HPLC법%유고%함량측정
Capsaicin%HPLC%Ointment%Content determination
用HPLC法以Phenomenex C18色谱柱（150mm×4.6mm）为固定相,甲醇-水（70∶30）为流动相，流速1.0ml·min-1，进样量20μl，检测波长为280 nm，按外标法以峰面积计算测定辣椒素乳膏中辣椒素的含量。结果辣椒素在52.308μg·ml-1～98.804μg·ml-1范围内线性关系良好，相关系数 r＝0.9999。平均回收率99.43%，RSD为0.85%。本法简便、准确、可靠,适用于辣椒素乳膏中辣椒素的含量测定。
用HPLC法以Phenomenex C18色譜柱（150mm×4.6mm）為固定相,甲醇-水（70∶30）為流動相，流速1.0ml·min-1，進樣量20μl，檢測波長為280 nm，按外標法以峰麵積計算測定辣椒素乳膏中辣椒素的含量。結果辣椒素在52.308μg·ml-1～98.804μg·ml-1範圍內線性關繫良好，相關繫數 r＝0.9999。平均迴收率99.43%，RSD為0.85%。本法簡便、準確、可靠,適用于辣椒素乳膏中辣椒素的含量測定。
용HPLC법이Phenomenex C18색보주（150mm×4.6mm）위고정상,갑순-수（70∶30）위류동상，류속1.0ml·min-1，진양량20μl，검측파장위280 nm，안외표법이봉면적계산측정랄초소유고중랄초소적함량。결과랄초소재52.308μg·ml-1～98.804μg·ml-1범위내선성관계량호，상관계수 r＝0.9999。평균회수솔99.43%，RSD위0.85%。본법간편、준학、가고,괄용우랄초소유고중랄초소적함량측정。
High performance liquid chromatography was performed on Phenomenex C18 column. The chromatographic conditions were as follows: methanol- water(70:30) as mobile phase, flow rate being 1.0 ml·min-1,the detecting wavelength at 280nm. Capsaicin had good linear relation within the range from 52.308μg·ml-1 to 98.804μg·ml-1 (r=0.9999). The average recovery was 99.43%, the repetitive RSD was 0.85%. The method is simple, rapid and accurate, which can be used for content determination of capsaicin in capsaicin ointment.
