中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2014年
7期
561-565
,共5页
聂晓敏%苏利霄%周雅婧%赵迎新%史冬梅%刘宇扬%周志明%周玉杰
聶曉敏%囌利霄%週雅婧%趙迎新%史鼕梅%劉宇颺%週誌明%週玉傑
섭효민%소리소%주아청%조영신%사동매%류우양%주지명%주옥걸
冠状动脉疾病%微小RNAs%侧支循环
冠狀動脈疾病%微小RNAs%側支循環
관상동맥질병%미소RNAs%측지순배
Coronary artery disease%MicroRNAs%Collateral circulation
目的:观察冠状动脉严重狭窄患者血浆中微小RNA-126(miR-126)水平与冠状动脉侧支循环( CCC)形成的关系,探讨miR-126是否可成为CCC形成的生物标志物。方法本研究为前瞻性设计,连续入选2012年6月至2013年1月在北京安贞医院心内科接受选择性冠状动脉造影检查,证实左前降支、左回旋支或右冠状动脉中单支血管狭窄≥95%的冠心病患者(冠心病组),根据Rentrop分级将患者分成2个亚组:侧支良好亚组(Rentrop分级≥2)(n=64),侧支不良亚组(Rentrop分级≤1)(n=56);另选取健康对照组30例。 RT-PCR检测血浆miR-126的表达水平,ELISA检测血清中血管内皮生长因子( VEGF)的浓度。采用Pearson相关分析和多因素logistic回归进行相关性分析,采用受试者工作特征( ROC)曲线分析血浆miR-126水平对CCC形成的辨别能力。结果侧支良好亚组的空腹血糖水平明显低于侧支不良亚组[(5.99±1.48)mmol/L 比(6.40±2.50)mmol/L,P=0.044]。冠心病组血浆miR-126(0.04±0.01比0.07±0.02,P=0.023)和血清VEGF水平[(2110±455)ng/L比(2574±450)ng/L,P=0.011]均低于健康对照组。侧支良好亚组miR-126和VEGF水平明显高于侧支不良亚组[miR-126:0.06±0.02比0.03±0.01,P=0.021;VEGF:(2549±614)ng/L比(1759±452)ng/L,P=0.008]。侧支良好亚组血液中miR-126与VEGF呈正相关(r=0.712,P=0.005),侧支不良亚组miR-126与VEGF无相关性(r=0.342,P=0.483)。 logistic 回归分析显示, miR-126(OR=2.145,95%CI 1.691~2.988,P=0.001)和VEGF(OR=1.279,95%CI 1.068~2.295, P=0.013)是CCC形成的独立预测因子。以血浆miR-126水平为检验变量,ROC曲线分析显示曲线下面积为0.951( P=0.002)。结论血浆miR-126水平与CCC形成呈显著正相关,是CCC形成的独立预测因素,可能成为判别CCC形成情况的一种血液标志物。
目的:觀察冠狀動脈嚴重狹窄患者血漿中微小RNA-126(miR-126)水平與冠狀動脈側支循環( CCC)形成的關繫,探討miR-126是否可成為CCC形成的生物標誌物。方法本研究為前瞻性設計,連續入選2012年6月至2013年1月在北京安貞醫院心內科接受選擇性冠狀動脈造影檢查,證實左前降支、左迴鏇支或右冠狀動脈中單支血管狹窄≥95%的冠心病患者(冠心病組),根據Rentrop分級將患者分成2箇亞組:側支良好亞組(Rentrop分級≥2)(n=64),側支不良亞組(Rentrop分級≤1)(n=56);另選取健康對照組30例。 RT-PCR檢測血漿miR-126的錶達水平,ELISA檢測血清中血管內皮生長因子( VEGF)的濃度。採用Pearson相關分析和多因素logistic迴歸進行相關性分析,採用受試者工作特徵( ROC)麯線分析血漿miR-126水平對CCC形成的辨彆能力。結果側支良好亞組的空腹血糖水平明顯低于側支不良亞組[(5.99±1.48)mmol/L 比(6.40±2.50)mmol/L,P=0.044]。冠心病組血漿miR-126(0.04±0.01比0.07±0.02,P=0.023)和血清VEGF水平[(2110±455)ng/L比(2574±450)ng/L,P=0.011]均低于健康對照組。側支良好亞組miR-126和VEGF水平明顯高于側支不良亞組[miR-126:0.06±0.02比0.03±0.01,P=0.021;VEGF:(2549±614)ng/L比(1759±452)ng/L,P=0.008]。側支良好亞組血液中miR-126與VEGF呈正相關(r=0.712,P=0.005),側支不良亞組miR-126與VEGF無相關性(r=0.342,P=0.483)。 logistic 迴歸分析顯示, miR-126(OR=2.145,95%CI 1.691~2.988,P=0.001)和VEGF(OR=1.279,95%CI 1.068~2.295, P=0.013)是CCC形成的獨立預測因子。以血漿miR-126水平為檢驗變量,ROC麯線分析顯示麯線下麵積為0.951( P=0.002)。結論血漿miR-126水平與CCC形成呈顯著正相關,是CCC形成的獨立預測因素,可能成為判彆CCC形成情況的一種血液標誌物。
목적:관찰관상동맥엄중협착환자혈장중미소RNA-126(miR-126)수평여관상동맥측지순배( CCC)형성적관계,탐토miR-126시부가성위CCC형성적생물표지물。방법본연구위전첨성설계,련속입선2012년6월지2013년1월재북경안정의원심내과접수선택성관상동맥조영검사,증실좌전강지、좌회선지혹우관상동맥중단지혈관협착≥95%적관심병환자(관심병조),근거Rentrop분급장환자분성2개아조:측지량호아조(Rentrop분급≥2)(n=64),측지불량아조(Rentrop분급≤1)(n=56);령선취건강대조조30례。 RT-PCR검측혈장miR-126적표체수평,ELISA검측혈청중혈관내피생장인자( VEGF)적농도。채용Pearson상관분석화다인소logistic회귀진행상관성분석,채용수시자공작특정( ROC)곡선분석혈장miR-126수평대CCC형성적변별능력。결과측지량호아조적공복혈당수평명현저우측지불량아조[(5.99±1.48)mmol/L 비(6.40±2.50)mmol/L,P=0.044]。관심병조혈장miR-126(0.04±0.01비0.07±0.02,P=0.023)화혈청VEGF수평[(2110±455)ng/L비(2574±450)ng/L,P=0.011]균저우건강대조조。측지량호아조miR-126화VEGF수평명현고우측지불량아조[miR-126:0.06±0.02비0.03±0.01,P=0.021;VEGF:(2549±614)ng/L비(1759±452)ng/L,P=0.008]。측지량호아조혈액중miR-126여VEGF정정상관(r=0.712,P=0.005),측지불량아조miR-126여VEGF무상관성(r=0.342,P=0.483)。 logistic 회귀분석현시, miR-126(OR=2.145,95%CI 1.691~2.988,P=0.001)화VEGF(OR=1.279,95%CI 1.068~2.295, P=0.013)시CCC형성적독립예측인자。이혈장miR-126수평위검험변량,ROC곡선분석현시곡선하면적위0.951( P=0.002)。결론혈장miR-126수평여CCC형성정현저정상관,시CCC형성적독립예측인소,가능성위판별CCC형성정황적일충혈액표지물。
Objective To explore the relationship between plasma microRNA 126 ( miR-126 ) level and coronary collateral circulation ( CCC) formation and to determine whether the miR-126 in plasma could serve as a blood-based biomarker for CCC in patients with severely narrowed coronary arteries ( CAD ).Methods In this prospective study , a total of 120 consecutive CAD patients with ≥95% stenosis in one epicardial coronary artery were enrolled.Thirty healthy people served as normal control.They were divided into two groups according to Rentrop grades:patients with grade 2 and 3 collateral development ( good CCC group, n=64) and patients with grade 0 and 1 collateral development (poor CCC group, n=56).Plasma miR-126 was measured by RT-PCR and serum VEGF was evaluated by ELISA method.Results Fasting plasma glucose ( FPG) was significantly lower in patients with good CCC than in patients with poor CCC ((5.99 ±1.48) mmol/L vs.(6.40 ±2.50) mmol/L).Plasma miR-126 levels and VEGF levels were significantly lower in CAD patients than in healthy people (0.04 ±0.01 vs.0.07 ±0.02, P=0.023 and (2 110 ±455) ng/L vs.(2 574 ±450) ng/L, P=0.011, respectively).miR-126 and VEGF levels were significantly higher in good CCC group than in poor CCC group ( miR-126:0.06 ±0.02 vs.0.03 ±0.01, P=0.021;VEGF:(2 549 ±614) ng/L vs.(1 759 ±452) ng/L, P=0.008).In CAD patients with good CCC, the miR-126 level was positively correlated to the VEGF expression (r =0.712,P=0.005) while there was no correlation between miR-126 level VEGF in CAD patients with poor CCC ( r =0.342, P =0.483).Multivariate analysis revealed that plasma miR-126 ( OR=2.145,95%CI 1.691 -2.988, P =0.001) and VEGF ( OR =1.279, 95%CI 1.068 -2.295, P =0.013 ) were independent predictors of collateral formation in patients with severely narrowed coronary arteries.In CAD patients , the area under the miR-126 ROC curve is 0.951 ( P=0.002).Conclusion Plasma miR-126 level is positively correlated to the CCC formation and is an independent predictor of CCC development in patients with severely narrowed coronary arteries , suggesting that plasma miR-126 might be a useful new , stable blood biomarker for predicting CCC formation in patients with severely narrowed coronary arteries.