CAJ | 학술논문

目的：探讨p120-连环蛋白在膀胱癌BIU-87和T24细胞株中的表达水平，分析p120-连环蛋白下调对膀胱癌BIU-87细胞增殖、侵袭和黏附能力的影响。方法2010年8月至2011年5月，采用实时定量PCR和蛋白质印迹技术检测膀胱癌BIU-87和T24细胞株中p120-连环蛋白的表达水平。将膀胱癌 BIU-87细胞分为3组：未转染组，转染p120-连环蛋白siRNA 组和转染control siRNA组，应用siRNA方法沉默BIU-87细胞中p120-连环蛋白基因表达，采用噻唑盐法和克隆形成实验检测p120-连环蛋白siRNA对BIU-87细胞生长率的影响，Transwell小室法检测BIU-87细胞侵袭能力的变化，细胞黏附人工重组基底膜实验检测BIU-87细胞黏附能力的变化。结果 p120-连环蛋白在膀胱癌BIU-87和T24细胞株中均有表达，在BIU-87细胞株中的表达水平（0．11±0．39）显著高于T24细胞株（0．48±0．17），差异有统计学意义（P＜0．05）。噻唑盐法检测BIU-87细胞的增殖能力，转染p120-连环蛋白siRNA组的生长增殖率（182．7±13．4）％显著高于转染control siRNA组的（95．5±9．9）％和未转染组的100．0％，差异有统计学意义（ P＜0．05）。转染p120-连环蛋白siRNA组BIU-87细胞侵袭数量[（217．5±15．9）个]明显高于转染 control siRNA 组[（105．4±10．1）个]和未转染组[（92．7±8．3）个]，差异有统计学意义（ P＜0．05）。转染 p120-连环蛋白 siRNA 组 BIU-87细胞的黏附能力（57．3±6．4）％明显低于转染control siRNA组（98．1±11．0）％和未转染组100．0％，差异有统计学意义（ P＜0．05）。结论 p120-连环蛋白在恶性度低的膀胱癌细胞中表达水平高，其表达下调可促进膀胱癌细胞的增殖和侵袭，抑制膀胱癌细胞的黏附。
목적：탐토p120-련배단백재방광암BIU-87화T24세포주중적표체수평，분석p120-련배단백하조대방광암BIU-87세포증식、침습화점부능력적영향。방법2010년8월지2011년5월，채용실시정량PCR화단백질인적기술검측방광암BIU-87화T24세포주중p120-련배단백적표체수평。장방광암 BIU-87세포분위3조：미전염조，전염p120-련배단백siRNA 조화전염control siRNA조，응용siRNA방법침묵BIU-87세포중p120-련배단백기인표체，채용새서염법화극륭형성실험검측p120-련배단백siRNA대BIU-87세포생장솔적영향，Transwell소실법검측BIU-87세포침습능력적변화，세포점부인공중조기저막실험검측BIU-87세포점부능력적변화。결과 p120-련배단백재방광암BIU-87화T24세포주중균유표체，재BIU-87세포주중적표체수평（0．11±0．39）현저고우T24세포주（0．48±0．17），차이유통계학의의（P＜0．05）。새서염법검측BIU-87세포적증식능력，전염p120-련배단백siRNA조적생장증식솔（182．7±13．4）％현저고우전염control siRNA조적（95．5±9．9）％화미전염조적100．0％，차이유통계학의의（ P＜0．05）。전염p120-련배단백siRNA조BIU-87세포침습수량[（217．5±15．9）개]명현고우전염 control siRNA 조[（105．4±10．1）개]화미전염조[（92．7±8．3）개]，차이유통계학의의（ P＜0．05）。전염 p120-련배단백 siRNA 조 BIU-87세포적점부능력（57．3±6．4）％명현저우전염control siRNA조（98．1±11．0）％화미전염조100．0％，차이유통계학의의（ P＜0．05）。결론 p120-련배단백재악성도저적방광암세포중표체수평고，기표체하조가촉진방광암세포적증식화침습，억제방광암세포적점부。
Objective To investigate the expression of p 120-catenin in human bladder cell line BIU-87 and T24.And to explore the effect of down-regulation of p120-catenin on proliferation, invasion and adhesion of BIU-87 cell line. Methods The study was from Aug .2010 to May.2011.Real-time RT-PCR and Western blot were used to examine the expression of p 120-catenin in human bladder cell line BIU-87 and T24.There were three study groups:non-transfected group , the group transfected with p 120-catenin siRNA and the group transfected with control siRNA .p120-catenin siRNA was used to decrease the expression of p120-catenin.MTT assay and colony formation assay were used to study the BIU-87 cell growth and cell pro-liferation.The invasion of BIU-87 cells was measured by Transwell chamber assay .Cell adhesion artificial re-constituted basement membrane assay was used to examine the change of BIU-87 cell adhesion capacity . Results In both BIU-87 and T24 cells there were the expressions of p 120-catenin.But the expression in BIU-87 cells (0.11±0.39) was more than that in T24 cells (0.48±0.17).The decreased of p120-catenin ex-pression could enhance the proliferation (182.7±13.4%) and the invasiveness (217.5±15.9) and decrease the adhesion capacity (57.3±6.4%) of BIU-87 cells. Conclusions There is higher expression level of p120-catenin in lower-grade malignant bladder cancer cells .The down-regulation of p120-catenin promotes the bladder cancer proliferation and invasiveness of bladder carcinoma cells , and inhibited the bladder canc-er cell adhesion .