CAJ | 학술논문

目的：探讨谷胱甘肽硫基转移酶μ-3（ glutathione S-transferase mu 3，GSTM3）在前列腺癌中的表达及意义。方法2012年9月至2013年9月应用二维荧光差异凝胶电泳技术筛选前列腺癌标本中的差异蛋白，并利用质谱分析验证前期表达谱基因芯片结果获得的GSTM3。选取28例行根治性前列腺切除术患者的病理标本。患者年龄＜60岁22例，≥60岁6例；PSA 水平＜4μg/L 5例，≥4μg/L 23例；Gleason评分＜7分26例，≥7分2例；TNM分期＜T2a期18例，≥T2a期10例。采用实时荧光定量PCR法检测前列腺癌组织及其癌旁组织中GSTM3的表达情况，分析GSTM3与患者临床病理参数的相关性。结果二维荧光差异凝胶电泳获得前列腺癌与癌旁组织的双向凝胶电泳图谱，结果显示GSTM3为差异表达的蛋白，GSTM3蛋白在前列腺癌组织中表达下调，与癌旁组织比较差异有统计学意义（ P＜0．05）。实时荧光定量PCR法检测结果显示，GSTM3在癌旁组织中的表达（8．12±0．51）显著高于癌组织（7．18±0．54），差异有统计学意义（P＜0．05）。 GSTM3的表达与患者的年龄、PSA水平、Gleason评分、TNM分期均无显著相关性（P＞0．05），在不同PSA水平分组中GSTM3表达差异无统计学意义（P＞0．05）。结论与癌旁组织比较，GSTM3在前列腺癌组织中表达下调，但GSTM3的表达与前列腺癌的恶性程度无关。
목적：탐토곡광감태류기전이매μ-3（ glutathione S-transferase mu 3，GSTM3）재전렬선암중적표체급의의。방법2012년9월지2013년9월응용이유형광차이응효전영기술사선전렬선암표본중적차이단백，병이용질보분석험증전기표체보기인심편결과획득적GSTM3。선취28례행근치성전렬선절제술환자적병리표본。환자년령＜60세22례，≥60세6례；PSA 수평＜4μg/L 5례，≥4μg/L 23례；Gleason평분＜7분26례，≥7분2례；TNM분기＜T2a기18례，≥T2a기10례。채용실시형광정량PCR법검측전렬선암조직급기암방조직중GSTM3적표체정황，분석GSTM3여환자림상병리삼수적상관성。결과이유형광차이응효전영획득전렬선암여암방조직적쌍향응효전영도보，결과현시GSTM3위차이표체적단백，GSTM3단백재전렬선암조직중표체하조，여암방조직비교차이유통계학의의（ P＜0．05）。실시형광정량PCR법검측결과현시，GSTM3재암방조직중적표체（8．12±0．51）현저고우암조직（7．18±0．54），차이유통계학의의（P＜0．05）。 GSTM3적표체여환자적년령、PSA수평、Gleason평분、TNM분기균무현저상관성（P＞0．05），재불동PSA수평분조중GSTM3표체차이무통계학의의（P＞0．05）。결론여암방조직비교，GSTM3재전렬선암조직중표체하조，단GSTM3적표체여전렬선암적악성정도무관。
Objective To explore the role and clinical significance of GSTM 3 ( glutathione S-trans-ferase mu 3) expression in prostate cancer (PCa). Methods We had used the two-dimensional fluores-cence difference gel electrophoresis ( 2D-DIGE) and mass spectral analysis to further verify the microarray data of mRNA expression profiling discovered .GSTM3 mRNA level was detected by Rael-time Quantitative PCR ( RT-QPCR) in 28 pairs of prostate cancer tissue and benign tissue .The relationship of GSTM 3 level with the serum PSA level and the clinical feature of PCa were analyzed . Results In 2D-DIGE study, we found that the expression of GSTM 3 protein in adjacent tissues was significantly higher than that in PCa tis-sues (P<0.05).RT-QPCR results showed that GSTM3 in adjacent tissues (8.12±0.51) was significantly higher than that in PCa tissues (7.18±0.54) (P<0.05).There was no significant difference of GSTM3 ex-pression in different serum PSA packets ( P>0.05) and prostate cancer clinical pathological parameters ( P>0.05). Conclusions GSTM3 expression is down-regulated in PCa tissues, and we may identify PCa by detecting the GSTM 3 expression .