中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2014年
7期
26-29
,共4页
苏锦明%黄颉刚%叶力%卢祥婵%蒋俊俊%梁冰玉%周波%廖艳研%李彧%潘沛江%陶人川%曹存巍%梁浩
囌錦明%黃頡剛%葉力%盧祥嬋%蔣俊俊%樑冰玉%週波%廖豔研%李彧%潘沛江%陶人川%曹存巍%樑浩
소금명%황힐강%협력%로상선%장준준%량빙옥%주파%료염연%리욱%반패강%도인천%조존외%량호
人类免疫缺陷病毒%分枝杆菌 ,结核%重叠感染%Toll 样受体 2%信号通路
人類免疫缺陷病毒%分枝桿菌 ,結覈%重疊感染%Toll 樣受體 2%信號通路
인류면역결함병독%분지간균 ,결핵%중첩감염%Toll 양수체 2%신호통로
Human immunodeficiency virus%Mycobacterium tuberculosis%Superinfection%Toll-like receptor 2%Signaling pathway
目的：探讨 Toll 样受体2（TLR2）及其信号通路下游因子在 HIV /结核分枝杆菌（M TB）双重感染者中的表达。方法选择 HIV 单纯感染者32例、HIV/M TB 双重感染者30例、M TB 单纯感染者28例，以及健康对照者29名作为观察对象。采集外周静脉血，检测血清 HIV 载量，实时定量 PCR （qPCR）检测 PBMC TLR2 mRNA 相对表达量，流式细胞术检测外周血单核细胞 TLR2平均荧光强度（MFI）表达水平，ELISA 法测定血清 IL-6、TNF-α。统计学处理采用卡方检验、t 检验、方差分析和秩和检验。结果 HIV /M TB 组的病毒载量为（5．113±1．018） lg 拷贝/mL ，HIV 组为（4．416±1．020） lg 拷贝/mL ，两组比较差异有统计学意义（t ＝3．449，P＜0．01）；HIV 组、HIV /M TB 组、M TB 组和健康对照组 PBMC TLR2 mRNA 相对表达量分别为1．397±0．601、1．463±0．702、1．429±0．630和0．970±0．488，4组间比较差异有统计学意义（ F ＝4．197，P ＝0．007）；4组单核细胞 TLR2的 MFI 分别为28．12±4．55、38．11±11．77、31．13±12．10和23．33±5．14，4组间比较差异有统计学意义（F＝13．976， P＜0．01）。4组血清 IL-6、TNF-α水平比较，差异有统计学意义（Z 值分别为19．088、15．475，均 P ＜0．01），3个病例组的 IL-6表达均比健康对照组高，而 TNF-α表达均比健康对照组低。结论 HIV /M TB 双重感染有可能加重 TLR2的活化，且促进 IL-6的表达上调。
目的：探討 Toll 樣受體2（TLR2）及其信號通路下遊因子在 HIV /結覈分枝桿菌（M TB）雙重感染者中的錶達。方法選擇 HIV 單純感染者32例、HIV/M TB 雙重感染者30例、M TB 單純感染者28例，以及健康對照者29名作為觀察對象。採集外週靜脈血，檢測血清 HIV 載量，實時定量 PCR （qPCR）檢測 PBMC TLR2 mRNA 相對錶達量，流式細胞術檢測外週血單覈細胞 TLR2平均熒光彊度（MFI）錶達水平，ELISA 法測定血清 IL-6、TNF-α。統計學處理採用卡方檢驗、t 檢驗、方差分析和秩和檢驗。結果 HIV /M TB 組的病毒載量為（5．113±1．018） lg 拷貝/mL ，HIV 組為（4．416±1．020） lg 拷貝/mL ，兩組比較差異有統計學意義（t ＝3．449，P＜0．01）；HIV 組、HIV /M TB 組、M TB 組和健康對照組 PBMC TLR2 mRNA 相對錶達量分彆為1．397±0．601、1．463±0．702、1．429±0．630和0．970±0．488，4組間比較差異有統計學意義（ F ＝4．197，P ＝0．007）；4組單覈細胞 TLR2的 MFI 分彆為28．12±4．55、38．11±11．77、31．13±12．10和23．33±5．14，4組間比較差異有統計學意義（F＝13．976， P＜0．01）。4組血清 IL-6、TNF-α水平比較，差異有統計學意義（Z 值分彆為19．088、15．475，均 P ＜0．01），3箇病例組的 IL-6錶達均比健康對照組高，而 TNF-α錶達均比健康對照組低。結論 HIV /M TB 雙重感染有可能加重 TLR2的活化，且促進 IL-6的錶達上調。
목적：탐토 Toll 양수체2（TLR2）급기신호통로하유인자재 HIV /결핵분지간균（M TB）쌍중감염자중적표체。방법선택 HIV 단순감염자32례、HIV/M TB 쌍중감염자30례、M TB 단순감염자28례，이급건강대조자29명작위관찰대상。채집외주정맥혈，검측혈청 HIV 재량，실시정량 PCR （qPCR）검측 PBMC TLR2 mRNA 상대표체량，류식세포술검측외주혈단핵세포 TLR2평균형광강도（MFI）표체수평，ELISA 법측정혈청 IL-6、TNF-α。통계학처리채용잡방검험、t 검험、방차분석화질화검험。결과 HIV /M TB 조적병독재량위（5．113±1．018） lg 고패/mL ，HIV 조위（4．416±1．020） lg 고패/mL ，량조비교차이유통계학의의（t ＝3．449，P＜0．01）；HIV 조、HIV /M TB 조、M TB 조화건강대조조 PBMC TLR2 mRNA 상대표체량분별위1．397±0．601、1．463±0．702、1．429±0．630화0．970±0．488，4조간비교차이유통계학의의（ F ＝4．197，P ＝0．007）；4조단핵세포 TLR2적 MFI 분별위28．12±4．55、38．11±11．77、31．13±12．10화23．33±5．14，4조간비교차이유통계학의의（F＝13．976， P＜0．01）。4조혈청 IL-6、TNF-α수평비교，차이유통계학의의（Z 치분별위19．088、15．475，균 P ＜0．01），3개병례조적 IL-6표체균비건강대조조고，이 TNF-α표체균비건강대조조저。결론 HIV /M TB 쌍중감염유가능가중 TLR2적활화，차촉진 IL-6적표체상조。
Objective To explore the expressions of Toll-like receptor 2 (TLR2 ) and the downstream proteins in patients with human immunodeficiency virus /Mycobacterium tuberculosis (HIV /M TB) co-infection .Methods A total of 119 subjects were randomly enrolled .The subjects were divided into four groups :HIV group (n = 32) ,HIV /M TB group (n = 30) ,M TB group (n = 28) and healthy control group (n= 29) .Peripheral venous blood was collected and the HIV-1 viral load was determined by standard method .The expression levels of TLR2 mRNA in peripheral blood mononuclear cells (PBMC) were determined by real-time quantitative PCR (qPCR) and mean fluorescent intensity (MFI) of TLR2 protein was detected by flow cytometry .The plasma interleukin (IL)-6 and tumor necrosis factor (TNF)-α levels were measured with enzyme-linked immunosorbent assay kits .The data were statistically analyzed by chi-square test ,students t test ,analysis of variance and rank sum test when appropriate .Results The viral load in HIV /M TB group ([5 .113 ± 1 .018] lg copy/mL ) was significantly higher than that in HIV group ([4 .416 ± 1 .020] lg copy/mL ; t = 3 .449 , P< 0 .01) .The TLR2 mRNA expressions in PBMC <br> among HIV ,HIV/M TB ,M TB and healthy control groups were 1 .397 ± 0 .601 ,1 .463 ± 0 .702 ,1 .429 ± 0 .630 ,and 0 .970 ± 0 .488 ,respectively ,which was significantly different among the 4 groups (F =4 .197 , P= 0 .007) .The MFI of TLR2 protein expressions on PBMC among HIV ,HIV /M TB ,M TB and healthy control groups were 28 .12 ± 4 .55 ,38 .11 ± 11 .77 ,31 .13 ± 12 .10 and 23 .33 ± 5 .14 ,respectively . The TLR2 protein expression levels were significantly different among 4 groups (F= 13 .976 ,P< 0 .01) . The plasma IL-6 and TNF-α concentrations were significantly different among 4 groups (Z = 19 .088 , 15 .475 ,both P< 0 .01) .The IL-6 concentrations in three patient groups were higher than that in healthy control group ,but the TNF-α concentrations were lower than healthy control group .Conclusions The co-infection of HIV-1 and M TB may enhance the activation of TLR2 signaling pathway ,which leads to the increased expression of IL-6 .
