CAJ | 학술논문

目的：检测真核表达质粒载体pIRES2-EGFP-NT3在阳离子脂质体介导下对新生小鼠耳蜗成纤维细胞的转染情况。方法培养新生小鼠耳蜗成纤维细胞，用阳离子脂质体Lipofectamine TM 2000介导的方法，将含有目的基因NT3的真核表达质粒载体pIRES2-EGFP-NT3对其进行转染，转染后24 h，通过Confocal 显微镜观察新生小鼠耳蜗成纤维细胞的转染情况。结果通过阳离子脂质体Lipofectamine TM 2000介导，真核表达质粒载体pIRES2-EGFP-NT3能有效转染新生小鼠耳蜗成纤维细胞。结论含有目的基因NT3的真核表达质粒载体pIRES2-EGFP-NT3在阳离子脂质体介导下对新生小鼠耳蜗成纤维细胞的有效转染为研究NT3基因转染对正常及致聋小鼠耳蜗效应的实验奠定了基础。
목적：검측진핵표체질립재체pIRES2-EGFP-NT3재양리자지질체개도하대신생소서이와성섬유세포적전염정황。방법배양신생소서이와성섬유세포，용양리자지질체Lipofectamine TM 2000개도적방법，장함유목적기인NT3적진핵표체질립재체pIRES2-EGFP-NT3대기진행전염，전염후24 h，통과Confocal 현미경관찰신생소서이와성섬유세포적전염정황。결과통과양리자지질체Lipofectamine TM 2000개도，진핵표체질립재체pIRES2-EGFP-NT3능유효전염신생소서이와성섬유세포。결론함유목적기인NT3적진핵표체질립재체pIRES2-EGFP-NT3재양리자지질체개도하대신생소서이와성섬유세포적유효전염위연구NT3기인전염대정상급치롱소서이와효응적실험전정료기출。
Objective To detect the transfection of pIRES 2-EGFP-NT3 in mouse cochlea fibroblast by cationic liposome . Methods After pIRES2-EGFP-NT3 had been abstracted successfully , it was transfected into mouse cochlea fibroblast by lipofectami-neTM2000.Twenty four hours later, the efficiency of the transfection was analyzed by confocal microscope .Results The pIRES2-EG-FP-NT3 was effectively transfected into mouse cochlea fibroblast by cationic liposome .The transfected fibroblasts displaying green fluo-rescence were observed under fluorescence microscope .Conclusions The effective transfection of pIRES 2-EGFP-NT3 into mouse cochlea fibroblast by lipofectamine TM 2000 laid the basis for the following experiments , such as NT3 gene transfection in deaf or normal cochlea and so on .