CAJ | 학술논문

建立一种适用于检测硒蛋白中硒代氨基酸的方法，使其应用于食品中营养强化剂硒蛋白的检测。用酶解硒蛋白；5mM 的柠檬酸铵加2%的甲醇溶液作为流动相，离子交换柱分离硒代氨基酸；ICP/MS检测硒代氨基酸的含量。用蛋白酶K、胰蛋白酶、链蛋白酶酶解，并用柠檬酸调节流动相的 pH 值（pH=4.9）能够成功分离SeCys、SeMet等硒代氨基酸。检出限分别为：3.5μg/kg、11.2μg/kg；线性范围为：以 Se计为0~200μg/L；加标回收率为：75%~120%。该方法能适用于硒蛋白中硒代氨基酸的分析。
건립일충괄용우검측서단백중서대안기산적방법，사기응용우식품중영양강화제서단백적검측。용매해서단백；5mM 적저몽산안가2%적갑순용액작위류동상，리자교환주분리서대안기산；ICP/MS검측서대안기산적함량。용단백매K、이단백매、련단백매매해，병용저몽산조절류동상적 pH 치（pH=4.9）능구성공분리SeCys、SeMet등서대안기산。검출한분별위：3.5μg/kg、11.2μg/kg；선성범위위：이 Se계위0~200μg/L；가표회수솔위：75%~120%。해방법능괄용우서단백중서대안기산적분석。
To establish a method for detection of selenium in selenium amino acid generation, detection and it can be used in food nutrition fortifier of selenoproteins. Solution of selenoprotein enzymes; 5mM methanol solution and ammonium citrate plus 2% as the mobile phase, ion exchange column chromatography of seleno amino acids; content of ICP/MS detection of seleno amino acids. Using proteinase K, trypsin, pronase enzyme solution with citric acid, and adjusting the pH value of the mobile phase (pH=4.9) to SeCys, SeMet seleno amino acid separation. The detection limits were:; the linear range is: Se 0~200μ g/L; recovery of standard addition: 75%~120%. Analysis of the method can be used in generation of selenoproteins in amino acid.