现代医院
現代醫院
현대의원
MODERN HOSPITAL
2014年
7期
72-75
,共4页
卵巢恶性肿瘤%miR-10a%转移%预后
卵巢噁性腫瘤%miR-10a%轉移%預後
란소악성종류%miR-10a%전이%예후
Ovarian malignant tumor%MiR-10a%Metastasis%Prognosis
目的：探讨miR-10a、miR-93和miR-200a在卵巢恶性肿瘤组织中的表达及其临床意义。方法采用实时定量PCR仪检测40例正常卵巢组织、40例卵巢良性囊肿组织和40例卵巢恶性肿瘤组织中miR-10a、miR-93和miR-200a表达情况，miR在不同组织中表达的比较采用方差分析，t检验分析miR与卵巢恶性肿瘤临床病理特征的关系，预后分析采用Kaplan-Meier法log-rank检验和Cox比例风险回归模型。结果 miR-10a在卵巢恶性肿瘤组织中的表达显著高于正常卵巢组织和卵巢良性囊肿组织（p＜0.001），miR-93和miR-200a在三组织中的表达差异均无统计学意义（p＞0.05）；miR-10a表达量在有大网膜转移、淋巴结转移和远处器官转移的卵巢恶性肿瘤组织中显著高于无转移者（p＜0.05）。卵巢恶性肿瘤组织miR-10a高表达的患者比低表达的患者中位生存时间更短，差异具有统计学意义（p＝0.01）；Cox模型多因素分析显示，miR-10a表达量是影响患者生存预后的独立因素（p＝0.002）。结论 miR-10a表达与卵巢癌转移有关，是影响卵巢癌预后的主要因素，可以作为判断卵巢癌预后的标志物。
目的：探討miR-10a、miR-93和miR-200a在卵巢噁性腫瘤組織中的錶達及其臨床意義。方法採用實時定量PCR儀檢測40例正常卵巢組織、40例卵巢良性囊腫組織和40例卵巢噁性腫瘤組織中miR-10a、miR-93和miR-200a錶達情況，miR在不同組織中錶達的比較採用方差分析，t檢驗分析miR與卵巢噁性腫瘤臨床病理特徵的關繫，預後分析採用Kaplan-Meier法log-rank檢驗和Cox比例風險迴歸模型。結果 miR-10a在卵巢噁性腫瘤組織中的錶達顯著高于正常卵巢組織和卵巢良性囊腫組織（p＜0.001），miR-93和miR-200a在三組織中的錶達差異均無統計學意義（p＞0.05）；miR-10a錶達量在有大網膜轉移、淋巴結轉移和遠處器官轉移的卵巢噁性腫瘤組織中顯著高于無轉移者（p＜0.05）。卵巢噁性腫瘤組織miR-10a高錶達的患者比低錶達的患者中位生存時間更短，差異具有統計學意義（p＝0.01）；Cox模型多因素分析顯示，miR-10a錶達量是影響患者生存預後的獨立因素（p＝0.002）。結論 miR-10a錶達與卵巢癌轉移有關，是影響卵巢癌預後的主要因素，可以作為判斷卵巢癌預後的標誌物。
목적：탐토miR-10a、miR-93화miR-200a재란소악성종류조직중적표체급기림상의의。방법채용실시정량PCR의검측40례정상란소조직、40례란소량성낭종조직화40례란소악성종류조직중miR-10a、miR-93화miR-200a표체정황，miR재불동조직중표체적비교채용방차분석，t검험분석miR여란소악성종류림상병리특정적관계，예후분석채용Kaplan-Meier법log-rank검험화Cox비례풍험회귀모형。결과 miR-10a재란소악성종류조직중적표체현저고우정상란소조직화란소량성낭종조직（p＜0.001），miR-93화miR-200a재삼조직중적표체차이균무통계학의의（p＞0.05）；miR-10a표체량재유대망막전이、림파결전이화원처기관전이적란소악성종류조직중현저고우무전이자（p＜0.05）。란소악성종류조직miR-10a고표체적환자비저표체적환자중위생존시간경단，차이구유통계학의의（p＝0.01）；Cox모형다인소분석현시，miR-10a표체량시영향환자생존예후적독립인소（p＝0.002）。결론 miR-10a표체여란소암전이유관，시영향란소암예후적주요인소，가이작위판단란소암예후적표지물。
Objective To study the expressions of miR-10a, miR-93 and miR-200a in malignant ovarian tumor tissues and their clinical significances.Methods Real-time quantitative PCR was used to detect the expression of 40 cases of normal ovarian tissue, 40 cases of benign ovarian cyst and 40 cases of miR-10a, miR-93 and miR-200a in malignant ovarian tumor tissues.Analysis of variance and t-Test were respectively applied to compare the expression of miR in different tissues and analyze the correlation between miR and clinicopathological characteristics of malignant ovarian tumor .Kaplan-Meier Log-rank test and Cox proportional hazards regression model were adopted in prognosis.Results The expression lev-els of miR-10a in ovarian cancer tissues were significantly higher than that in benign ovarian tumor tissues and normal ovari-an tissues (p<0.001), whereas the expression of miR-93 and miR-200a in the three types of tissues was no statistically significant difference (p>0.05).The expression quantity of miR-10a in malignant ovarian tumor tissues with greater omen-tum metastasis, lymph node metastasis and distant organ metastasis was significantly higher than that without metastasis ( p<0.05).The median survival time of patients with higher expression of miR -10a was lower than that of patients with low ex-pression of miR-10a (p=0.01).Multi-factor Cox model analysis showed that the expression quantity of miR -10a was an independent factor affecting survival prognosis in patients(p=0.002).Conclusion Our data suggest that miR-10a is asso-ciated with ovarian cancer metastasis, which is the main factor affecting prognosis in ovarian cancer.It might serve as a bio-marker for judging the prognosis in ovarian cancer.
