牙体牙髓牙周病学杂志
牙體牙髓牙週病學雜誌
아체아수아주병학잡지
CHINESE JOURNAL OF CONSERVATIVE DENTISTRY
2014年
7期
381-384
,共4页
茶黄素(TF)%巯基蛋白酶(CPs)%去磷酸化牛乳β-酪蛋白(Dβ-CN)%消散因子石英晶体微
茶黃素(TF)%巰基蛋白酶(CPs)%去燐痠化牛乳β-酪蛋白(Dβ-CN)%消散因子石英晶體微
다황소(TF)%구기단백매(CPs)%거린산화우유β-락단백(Dβ-CN)%소산인자석영정체미
theaflavin(TF)%cysteine proteases(CPs)%dephosphorylated bovine β-casein(Dβ-CN)%quartz crystal microbalance with dissipation(QCM-D)
目的：观察蛋白质/色素复合膜和蛋白质单层膜分别经木瓜蛋白酶、菠萝蛋白酶、无花果蛋白酶3种巯基蛋白酶（CPs）水解后再吸附色素的能力。方法：在消散因子石英微天平（QCM-D）芯片表面分别自组装构建去磷酸化牛乳β-酪蛋白（Dβ-CN）/茶黄素（TF）复合膜和Dβ-CN单层膜，经3种CPs水解后，观察残余膜的厚度及其再吸附TF的质量；用SPSS 18．0软件对结果进行单因素方差分析，非线性回归法拟合曲线分析膜厚度与TF吸附量的相关性。结果：Dβ-CN/TF复合膜和Dβ-CN单层膜分别经3种CPs水解后，其厚度均明显减少（P＜0．05）；残余膜再吸附TF的量也显著下降（P＜0．05），膜厚度与其吸附TF的质量呈非线性正相关。结论：巯基蛋白酶可降低蛋白质残余膜与TF亲和力，在清除和预防蛋白质色渍方面具有应用价值。
目的：觀察蛋白質/色素複閤膜和蛋白質單層膜分彆經木瓜蛋白酶、菠蘿蛋白酶、無花果蛋白酶3種巰基蛋白酶（CPs）水解後再吸附色素的能力。方法：在消散因子石英微天平（QCM-D）芯片錶麵分彆自組裝構建去燐痠化牛乳β-酪蛋白（Dβ-CN）/茶黃素（TF）複閤膜和Dβ-CN單層膜，經3種CPs水解後，觀察殘餘膜的厚度及其再吸附TF的質量；用SPSS 18．0軟件對結果進行單因素方差分析，非線性迴歸法擬閤麯線分析膜厚度與TF吸附量的相關性。結果：Dβ-CN/TF複閤膜和Dβ-CN單層膜分彆經3種CPs水解後，其厚度均明顯減少（P＜0．05）；殘餘膜再吸附TF的量也顯著下降（P＜0．05），膜厚度與其吸附TF的質量呈非線性正相關。結論：巰基蛋白酶可降低蛋白質殘餘膜與TF親和力，在清除和預防蛋白質色漬方麵具有應用價值。
목적：관찰단백질/색소복합막화단백질단층막분별경목과단백매、파라단백매、무화과단백매3충구기단백매（CPs）수해후재흡부색소적능력。방법：재소산인자석영미천평（QCM-D）심편표면분별자조장구건거린산화우유β-락단백（Dβ-CN）/다황소（TF）복합막화Dβ-CN단층막，경3충CPs수해후，관찰잔여막적후도급기재흡부TF적질량；용SPSS 18．0연건대결과진행단인소방차분석，비선성회귀법의합곡선분석막후도여TF흡부량적상관성。결과：Dβ-CN/TF복합막화Dβ-CN단층막분별경3충CPs수해후，기후도균명현감소（P＜0．05）；잔여막재흡부TF적량야현저하강（P＜0．05），막후도여기흡부TF적질량정비선성정상관。결론：구기단백매가강저단백질잔여막여TF친화력，재청제화예방단백질색지방면구유응용개치。
AIM:To determine the pigment reabsorption ability of protein/pigment complex layer and pro-tein monolayer after being hydrolyzed respectively by three kinds of cysteine proteases (CPs).METHODS:The de-phosphorylated bovine β-casein (Dβ-CN)/theaflavin (TF)complex layer and Dβ-CN monolayer were established self-assembly on a sensor surface of quartz crystal microbalance with dissipation (QCM-D),respectively.After both categories of layers had been hydrolyzed by cysteine protease papain,bomelian,and ficin respectively,the thickness of residual layers and the mass of TF re-adsorbed on the sufaces were measured.The data were analyzed by one-way AVNOA and SNK-q test,then were fitted by a nonlinear regression method.RESULTS:The thickness of Dβ-CN/TF complex layer and Dβ-CN monolayer were decreased after treatment with the 3 kinds of CPs (P<0.05).The mass of re-adsorpted TF on the surface of residual layer was reduced along with the decreases of layer thickness (P<0.05), the effects whowed a positive nonlinear corelation.CONCLUSION:Hydrolyzation by CPs can decrease the affinity be-tween TF and residual complex layer or monolayer.CPs may be used for proteinaceous tooth stain removal and preven-tion.
