CAJ | 학술논문

目的：研究芹菜素（apigenin）对变异链球菌致龋力的影响。方法：用含20 g/L蔗糖的TPY液体培养基，以二倍体稀释法将芹菜素配制成12．5、25、50、100、200、400μg/mL 的含药培养基，并与变异链球菌ATCC25175（血清C型）共同培养；分别观察不同浓度芹菜素对变异链球菌增殖及其产酸能力、粘附能力、葡聚糖转移酶（GTF ）活力和水不溶性胞外多糖生成能力的影响。结果：与空白对照组相比，不同浓度芹菜素对变异链球菌的增殖和产酸均无明显影响（P＞0．05）；但可明显抑制变异链球菌的粘附能力、GTF的活性以及水不溶性胞外多糖的生成量（P＜0．05）；且随着芹菜素浓度的增加，其抑制作用逐渐增强，各浓度组相比，除200μg/mL与400μg/mL组间无统计学差异（P＞0．05）外，其他各浓度组间差异均有统计学意义（P＜0．05）。结论：芹菜素对变异链球菌的生长、产酸无影响，但对其粘附、GTF活性和水不溶性胞外多糖的生成具有抑制作用。
목적：연구근채소（apigenin）대변이련구균치우력적영향。방법：용함20 g/L자당적TPY액체배양기，이이배체희석법장근채소배제성12．5、25、50、100、200、400μg/mL 적함약배양기，병여변이련구균ATCC25175（혈청C형）공동배양；분별관찰불동농도근채소대변이련구균증식급기산산능력、점부능력、포취당전이매（GTF ）활력화수불용성포외다당생성능력적영향。결과：여공백대조조상비，불동농도근채소대변이련구균적증식화산산균무명현영향（P＞0．05）；단가명현억제변이련구균적점부능력、GTF적활성이급수불용성포외다당적생성량（P＜0．05）；차수착근채소농도적증가，기억제작용축점증강，각농도조상비，제200μg/mL여400μg/mL조간무통계학차이（P＞0．05）외，기타각농도조간차이균유통계학의의（P＜0．05）。결론：근채소대변이련구균적생장、산산무영향，단대기점부、GTF활성화수불용성포외다당적생성구유억제작용。
AIM:To study the effects of apigenin on the cariogenicity of Streptoccous mutans (S.mutans). METHODS:S.mutans ATCC25175 cells were treated by apigenin at the concentration (μg/mL)of 12.5,25,50, 100,200 and 400 respectively,and TPY liquid medium was used as the control.Bacteriostatic experiment was conduc-ted by disc diffusion method.PH value drop quantity,adhesion inhibition rate,glucanotransferase (GTF)vitality and the content of insoluble exopolysaccharides were measured in every groups.RESULTS:Apigenin solution at the vari-ous concentrations showed no effect on the proliferation and anaerobic acidogenesis of the bacteria.With the increase of apigenin concentration the adhesion inhibition rate of S.mutans increased,while GTF vitality and insoluble exopolysac-charides content decreased(P<0.05).CONCLUSION:Apigenin has no inhibition effect on the proliferation and acid production of S.mutans.However,it can inhibit the adhesion ability,GTF enzymes activity and insoluble exopo-lysaccharides production of S.mutans.