世界中西医结合杂志
世界中西醫結閤雜誌
세계중서의결합잡지
WORLD JOURNAL OF TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
7期
703-705,731
,共4页
赵伟峰%周明学%翟雅婷%王宁%底婷婷%李萍
趙偉峰%週明學%翟雅婷%王寧%底婷婷%李萍
조위봉%주명학%적아정%왕저%저정정%리평
银屑病%普萘洛尔%基质配方%优化造模
銀屑病%普萘洛爾%基質配方%優化造模
은설병%보내락이%기질배방%우화조모
Psoriasis%Propranolo%MatrixFormula%OptimizingModeling
目的：比较不同基质配方的普萘洛尔对豚鼠耳背部皮肤银屑病模型的干预作用。方法将24只豚鼠随机分为4组：空白对照组、模型基质组1、模型基质组2、模型基质组3，每组6只。空白对照组，双耳外侧每日外涂凡士林软膏，模型组用不同基质配方的5％的普萘洛尔软膏均匀涂于豚鼠双耳耳背部，每日涂3次，共20 d。10 d、20 d后分别对豚鼠耳背部皮肤进行数码相机拍照和采用游标卡尺测量耳部厚度。处死，取左耳部皮肤做病理。结果不同基质配方5％普萘洛尔干预10 d后，三组模型基质组豚鼠耳背部皮肤出现明显红斑、红点，部分出现结痂，以模型基质组2最为明显。干预20 d后，豚鼠耳背部皮肤上述现象已不十分明显。不同基质配方的5％普萘洛尔干预10 d后，与空白对照组比较，3组模型基质组豚鼠耳背部皮肤厚度明显增加，以模型基质组2增加最为明显（P＜0．05）。5％普萘洛尔干预20 d后，与空白对照组比较，3组模型基质组豚鼠耳背部皮肤厚度明显增加，以模型基质组1和2增加最为明显（P＜0．05）。不同基质配方的5％普萘洛尔干预20 d后，与空白对照组比较，3组模型基质组豚鼠耳背部皮肤均出现表皮增厚，明显的角化不全或角化过度，颗粒层变薄或不连续，颗粒细胞减少，棘层肥厚，真表皮交界处呈波浪状起伏。真皮层见有少量炎症细胞浸润。模型基质组2病理改变最为明显。结论不同基质配方的普萘洛尔对豚鼠耳背部皮肤均可造成银屑病模型，以5％普萘洛尔（含透皮剂）＋35％ PVA（成膜材料）组效果最为明显。随着干预时间的延长，病变有减轻的倾向，说明这种造模方法模拟的是银屑病急性期的病理改变。
目的：比較不同基質配方的普萘洛爾對豚鼠耳揹部皮膚銀屑病模型的榦預作用。方法將24隻豚鼠隨機分為4組：空白對照組、模型基質組1、模型基質組2、模型基質組3，每組6隻。空白對照組，雙耳外側每日外塗凡士林軟膏，模型組用不同基質配方的5％的普萘洛爾軟膏均勻塗于豚鼠雙耳耳揹部，每日塗3次，共20 d。10 d、20 d後分彆對豚鼠耳揹部皮膚進行數碼相機拍照和採用遊標卡呎測量耳部厚度。處死，取左耳部皮膚做病理。結果不同基質配方5％普萘洛爾榦預10 d後，三組模型基質組豚鼠耳揹部皮膚齣現明顯紅斑、紅點，部分齣現結痂，以模型基質組2最為明顯。榦預20 d後，豚鼠耳揹部皮膚上述現象已不十分明顯。不同基質配方的5％普萘洛爾榦預10 d後，與空白對照組比較，3組模型基質組豚鼠耳揹部皮膚厚度明顯增加，以模型基質組2增加最為明顯（P＜0．05）。5％普萘洛爾榦預20 d後，與空白對照組比較，3組模型基質組豚鼠耳揹部皮膚厚度明顯增加，以模型基質組1和2增加最為明顯（P＜0．05）。不同基質配方的5％普萘洛爾榦預20 d後，與空白對照組比較，3組模型基質組豚鼠耳揹部皮膚均齣現錶皮增厚，明顯的角化不全或角化過度，顆粒層變薄或不連續，顆粒細胞減少，棘層肥厚，真錶皮交界處呈波浪狀起伏。真皮層見有少量炎癥細胞浸潤。模型基質組2病理改變最為明顯。結論不同基質配方的普萘洛爾對豚鼠耳揹部皮膚均可造成銀屑病模型，以5％普萘洛爾（含透皮劑）＋35％ PVA（成膜材料）組效果最為明顯。隨著榦預時間的延長，病變有減輕的傾嚮，說明這種造模方法模擬的是銀屑病急性期的病理改變。
목적：비교불동기질배방적보내락이대돈서이배부피부은설병모형적간예작용。방법장24지돈서수궤분위4조：공백대조조、모형기질조1、모형기질조2、모형기질조3，매조6지。공백대조조，쌍이외측매일외도범사림연고，모형조용불동기질배방적5％적보내락이연고균균도우돈서쌍이이배부，매일도3차，공20 d。10 d、20 d후분별대돈서이배부피부진행수마상궤박조화채용유표잡척측량이부후도。처사，취좌이부피부주병리。결과불동기질배방5％보내락이간예10 d후，삼조모형기질조돈서이배부피부출현명현홍반、홍점，부분출현결가，이모형기질조2최위명현。간예20 d후，돈서이배부피부상술현상이불십분명현。불동기질배방적5％보내락이간예10 d후，여공백대조조비교，3조모형기질조돈서이배부피부후도명현증가，이모형기질조2증가최위명현（P＜0．05）。5％보내락이간예20 d후，여공백대조조비교，3조모형기질조돈서이배부피부후도명현증가，이모형기질조1화2증가최위명현（P＜0．05）。불동기질배방적5％보내락이간예20 d후，여공백대조조비교，3조모형기질조돈서이배부피부균출현표피증후，명현적각화불전혹각화과도，과립층변박혹불련속，과립세포감소，극층비후，진표피교계처정파랑상기복。진피층견유소량염증세포침윤。모형기질조2병리개변최위명현。결론불동기질배방적보내락이대돈서이배부피부균가조성은설병모형，이5％보내락이（함투피제）＋35％ PVA（성막재료）조효과최위명현。수착간예시간적연장，병변유감경적경향，설명저충조모방법모의적시은설병급성기적병리개변。
Objective Tocomparetheinterventioneffectonpsoriasismodelofauriculardorsalskin inguineapigstreatedwithPropranoloofdifferentmatrixformulas.Methods Twentyfourguineapigswere randomized into 4 groups,named a blank control group,a model matrix group 1 (group No.1 ),a model matrix group 2(group No.2)and a model matrix group 3(group No.3),6 guinea pigs in each one.In the blank con-trol group,Vaseline ointment was used externally on the affected areas every day.In the model groups,5%Propranolo ointment of different matrix formulas were applied,3 times a day,for 20 d totally.In 10 d and 20 d,the affected areas were pictured with digital camera and the vernier caliper was used to measure the ear thickness.After sacrificed,the skin on the back of the left ear was collected for pathological observation.Re-sults In10dof5%Propranoloointmentofdifferentmatrixformulas,theredspotsandrashesappearedon the affected skin in the three model groups and scab presented in some cases,which was the most obvious in the group No.2.In the intervention of 20 d,the above symptoms were not obvious.Compared with the blank control group,in 10 d,the thickness of the affected skin was increased apparently in the three model groups, in which,the thickening was the most apparent in the group No.2 (P<0.05 ).In 20 d,compared with the blank control group,the thickness of the affected skin was increased apparently in the three model groups,in which,the thickening was the most apparent in the group No.1 and the group No.2(P<0.05).In 20 d of intervention,compared with the blank control group,the skin of the affected area was thickening,with appar-ent para-aceratosis or hyperkeratosis;the granule layer was thinning or broken,the granule cells were re-duced,acanthosis occurred,the junction of epidermis and corium was wavy.There were little amount of in-flammatory cell infiltration in corium.The most obvious pathological changes were found in the group No.2. Conclusion Propranoloofdifferentmatrixformulascaninducepsoriasismodel,andtheresultisthebest with 5% Propranolo(containing transdermal agent)+35% PVA(film -forming material).With interven-tion,the lesion is alleviated in tendency,explaining that this modeling method simulates the acute pathological changes of psoriasis.
