湖南大学学报（自然科学版）
湖南大學學報（自然科學版）
호남대학학보（자연과학판）
JOURNAL OF HUNAN UNIVERSITY(NATURAL SCIENCES EDITION)
2014年
7期
70-77
,共8页
生物强化除磷%低温%好氧聚磷菌%反硝化聚磷菌%荧光原位杂交
生物彊化除燐%低溫%好氧聚燐菌%反硝化聚燐菌%熒光原位雜交
생물강화제린%저온%호양취린균%반초화취린균%형광원위잡교
enhanced biological phosphorus removal%low temperature%aerobic phosphorus removal microorganism%denitrifying phosphorus removal microorganism%fluorescence in situ hybridization
针对低温环境下生物强化除磷工艺的启动与运行,研究了厌氧/好氧和厌氧/缺氧两种模式富集驯化好氧聚磷菌和反硝化聚磷菌的效果.研究表明,以城市污水处理厂活性污泥为接种污泥,在8~11℃的低温环境下能有效完成好氧和反硝化聚磷菌的富集驯化,厌氧/好氧和厌氧/缺氧反应器分别在第40 d和第80 d达到稳定状态.厌氧/好氧反应器内污泥释磷和吸磷能力强于厌氧/缺氧反应器内污泥,分别为27.7 mg P/g MLVSS,35.2 mg P/g MLVSS,17.4 mg P/g MLVSS,23.1 mg P/g MLVSS.反硝化聚磷菌可以在好氧条件下以氧为电子受体快速吸收磷,而好氧聚磷菌在缺氧环境中以硝酸盐为电子受体立即吸收磷的能力较弱,仅为6.9 mgP/gMLVSS,占好氧吸磷的19.6%.厌氧/好氧和厌氧/缺氧两个反应器富集前后聚磷菌(Accumulibacter)的丰度分别由9.3%(接种污泥)增加到79.3%(好氧聚磷菌)和61.6%(反硝化聚磷菌),同样表明了在该低温环境下两个生物强化除磷工艺均实现了Accumulibacter的有效富集.
針對低溫環境下生物彊化除燐工藝的啟動與運行,研究瞭厭氧/好氧和厭氧/缺氧兩種模式富集馴化好氧聚燐菌和反硝化聚燐菌的效果.研究錶明,以城市汙水處理廠活性汙泥為接種汙泥,在8~11℃的低溫環境下能有效完成好氧和反硝化聚燐菌的富集馴化,厭氧/好氧和厭氧/缺氧反應器分彆在第40 d和第80 d達到穩定狀態.厭氧/好氧反應器內汙泥釋燐和吸燐能力彊于厭氧/缺氧反應器內汙泥,分彆為27.7 mg P/g MLVSS,35.2 mg P/g MLVSS,17.4 mg P/g MLVSS,23.1 mg P/g MLVSS.反硝化聚燐菌可以在好氧條件下以氧為電子受體快速吸收燐,而好氧聚燐菌在缺氧環境中以硝痠鹽為電子受體立即吸收燐的能力較弱,僅為6.9 mgP/gMLVSS,佔好氧吸燐的19.6%.厭氧/好氧和厭氧/缺氧兩箇反應器富集前後聚燐菌(Accumulibacter)的豐度分彆由9.3%(接種汙泥)增加到79.3%(好氧聚燐菌)和61.6%(反硝化聚燐菌),同樣錶明瞭在該低溫環境下兩箇生物彊化除燐工藝均實現瞭Accumulibacter的有效富集.
침대저온배경하생물강화제린공예적계동여운행,연구료염양/호양화염양/결양량충모식부집순화호양취린균화반초화취린균적효과.연구표명,이성시오수처리엄활성오니위접충오니,재8~11℃적저온배경하능유효완성호양화반초화취린균적부집순화,염양/호양화염양/결양반응기분별재제40 d화제80 d체도은정상태.염양/호양반응기내오니석린화흡린능력강우염양/결양반응기내오니,분별위27.7 mg P/g MLVSS,35.2 mg P/g MLVSS,17.4 mg P/g MLVSS,23.1 mg P/g MLVSS.반초화취린균가이재호양조건하이양위전자수체쾌속흡수린,이호양취린균재결양배경중이초산염위전자수체립즉흡수린적능력교약,부위6.9 mgP/gMLVSS,점호양흡린적19.6%.염양/호양화염양/결양량개반응기부집전후취린균(Accumulibacter)적봉도분별유9.3%(접충오니)증가도79.3%(호양취린균)화61.6%(반초화취린균),동양표명료재해저온배경하량개생물강화제린공예균실현료Accumulibacter적유효부집.
The acclimation of microorganisms responsible for phosphorus removal consisting of PAO and DPB was investigated by using two (enhanced biological phosphorus removal)EBPR reactors running anaerobic/aerobic (RAO) and anaerobic/anoxic (RAA)respectively to demonstrate the applicability of EBPR to wastewater treatment at low temperature.The results have shown that,at the temperature of 8~1 1 ℃,phosphorus removal microorganisms (Ac-cumulibacter)have been completely enriched in both anaerobic/aerobic and anaerobic/anoxic reactors after 40 and 80 days of operation,respectively.It has been found that the capacities of phosphorus release and uptake by PAO are higher than that by DPB,presenting the ratios of phosphorus release to MLVSS and phosphorus uptake to MLVSS of 27.7 mg P/g MLVSS,35.2 mg P/g MLVSS in RAO and 17.4 mg P/g MLVSS,23.1 mg P/g MLVSS in RAA,re-spectively.Moreover,DPB can immediately use oxygen as the electron acceptor for uptake phosphorus when given an aerobic condition,while PAO does not rapidly use nitrate as the electron acceptor when given an anoxic environment with the phosphorus uptake per MLVSS of 6.9 mg P/g MLVSS only accounting for 19.6% of that in aerobic condi-tion.Fluorescence in situ hybridization (FISH)analyses has shown that PAO (79.3% of all bacteria)and DPB (61. 6% of all bacteria)are dominant in their respective reactors,significantly greater than that in seed sludge (9.3%of all bacteria),which also demonstrates that microorganisms responsible for phosphorus removal have been effectively en-riched in the two EBPR systems proposed.
