中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
12期
2569-2571
,共3页
贺宏勇%陈伟东%汪学非%刘凤林%沈振斌%孙益红
賀宏勇%陳偉東%汪學非%劉鳳林%瀋振斌%孫益紅
하굉용%진위동%왕학비%류봉림%침진빈%손익홍
胃癌%分化%β1,6 N-乙酰氨基葡萄糖转移酶V
胃癌%分化%β1,6 N-乙酰氨基葡萄糖轉移酶V
위암%분화%β1,6 N-을선안기포도당전이매V
Stomach carcinoma%Differentiation%β1,6 N-acetylglucosaminyltransferase V
目的 探讨β1,6N-乙酰氨基葡萄糖转移酶V(MGAT5)与胃癌分化程度的相关性.方法 逆转录-聚合酶链反应(RT-PCR)和Western blot法检测胃癌细胞株中MGAT5的表达.收集2001年1月至2008年12月在复旦大学中山医院行胃癌根治术的213例胃腺癌与97例癌旁病理组织标本,免疫组织化学染色检测MGAT5的表达,并分析其与胃癌分化程度的相关性.结果 随着分化程度的下降,胃癌细胞株与胃癌组织中MGAT5的表达水平逐渐降低.MGAT5在低分化胃癌组织中的表达显著低于癌旁组织和高、中分化胃癌组织,差异有统计学意义(免疫组织化学染色评分:6.88±3.54比11.13 ±0.78、10.11±1.05、9.08 ±3.23,P＜0.001).结论 胃癌细胞株与胃癌组织中MGAT5的低表达与胃癌去分化程度显著相关.
目的 探討β1,6N-乙酰氨基葡萄糖轉移酶V(MGAT5)與胃癌分化程度的相關性.方法 逆轉錄-聚閤酶鏈反應(RT-PCR)和Western blot法檢測胃癌細胞株中MGAT5的錶達.收集2001年1月至2008年12月在複旦大學中山醫院行胃癌根治術的213例胃腺癌與97例癌徬病理組織標本,免疫組織化學染色檢測MGAT5的錶達,併分析其與胃癌分化程度的相關性.結果 隨著分化程度的下降,胃癌細胞株與胃癌組織中MGAT5的錶達水平逐漸降低.MGAT5在低分化胃癌組織中的錶達顯著低于癌徬組織和高、中分化胃癌組織,差異有統計學意義(免疫組織化學染色評分:6.88±3.54比11.13 ±0.78、10.11±1.05、9.08 ±3.23,P＜0.001).結論 胃癌細胞株與胃癌組織中MGAT5的低錶達與胃癌去分化程度顯著相關.
목적 탐토β1,6N-을선안기포도당전이매V(MGAT5)여위암분화정도적상관성.방법 역전록-취합매련반응(RT-PCR)화Western blot법검측위암세포주중MGAT5적표체.수집2001년1월지2008년12월재복단대학중산의원행위암근치술적213례위선암여97례암방병리조직표본,면역조직화학염색검측MGAT5적표체,병분석기여위암분화정도적상관성.결과 수착분화정도적하강,위암세포주여위암조직중MGAT5적표체수평축점강저.MGAT5재저분화위암조직중적표체현저저우암방조직화고、중분화위암조직,차이유통계학의의(면역조직화학염색평분:6.88±3.54비11.13 ±0.78、10.11±1.05、9.08 ±3.23,P＜0.001).결론 위암세포주여위암조직중MGAT5적저표체여위암거분화정도현저상관.
Objective To explore the expression of β1,6 N-acetylglucosaminyltransferase V (MGAT5) in human gastric cancer cell lines and tissues and its correlation with the differentiation of gastric cancer.Methods The protein level of MGAT5 in human gastric cancer cell lines was detected by using reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.The present study included a total of 213 patients with primary gastric adenocarcinoma who had undergone curative gastrectomy at Zhongshan Hospital,Fudan University between January 2001 and December 2008 without any chemotherapy or radiation therapy before surgery.Specimens of gastric cancer (n =213) and peritumor (n =97) tissues were stained using immunohistochemistry to determine the expression of MGAT5.Results The mRNA and protein levels of MGAT5 expression was higher in N87 (well differentiated gastric cancer cell line) and SGC7901 (moderately differentiated gastric cancer cell line) than in AGS (poorly differentiated gastric cancer cell line) and HGC27 (undifferentiated gastric cancer cell line).The expression of MGAT5 was decreased gradually with the decreased differentiation of gastric cancer.There was significant correlation between MGAT5 expression and differentiation of gastric cancer (Immunohistochemistry score:6.88 ± 3.54vs.11.13±0.78,10.11 ±l.05,9.08±3.23;P＜0.001).Conclusion The low expression of MGAT5 was significantly correlated with the dedifferentiation of gastric cancer.
