CAJ | 학술논문

目的：明确糖尿病小鼠血清TNF-α升高与肾脏脂质代谢的关系。方法 CD1小鼠按体重腹腔注射链脲佐菌素(150 mg·kg-1),72 h后空腹血糖大于16.7 mmol·L-1者确定为1型糖尿病模型,饲养1个月后,采用ELISA方法检测血清中TNF-α水平,免疫组织化学和Western blot方法检测SREBP-1和ADRP的表达。结果与正常对照小鼠相比,糖尿病小鼠血清 TNF-α水平明显升高,为正常对照小鼠的7.73倍。 SREBP-1和ADRP主要表达于肾小管上皮细胞,糖尿病小鼠肾脏SREBP-1前体片段、成熟片段和 ADRP表达明显高于正常对照小鼠,分别是对照组的2.31倍、1.74倍和1.72倍。小鼠血清TNF-α水平和肾脏ADRP表达的相关性分析证实二者间存在正相关,相关系数为0.914。结论糖尿病小鼠血清TNF-α升高可能是导致肾脏脂质积聚的因素之一。
목적：명학당뇨병소서혈청TNF-α승고여신장지질대사적관계。방법 CD1소서안체중복강주사련뇨좌균소(150 mg·kg-1),72 h후공복혈당대우16.7 mmol·L-1자학정위1형당뇨병모형,사양1개월후,채용ELISA방법검측혈청중TNF-α수평,면역조직화학화Western blot방법검측SREBP-1화ADRP적표체。결과여정상대조소서상비,당뇨병소서혈청 TNF-α수평명현승고,위정상대조소서적7.73배。 SREBP-1화ADRP주요표체우신소관상피세포,당뇨병소서신장SREBP-1전체편단、성숙편단화 ADRP표체명현고우정상대조소서,분별시대조조적2.31배、1.74배화1.72배。소서혈청TNF-α수평화신장ADRP표체적상관성분석증실이자간존재정상관,상관계수위0.914。결론당뇨병소서혈청TNF-α승고가능시도치신장지질적취적인소지일。
Aim Todeterminetherelationshipbe-tween serum TNF-α and renal abnormal lipid metabo-lismindiabeticmice.Methods CD1micewerein-jected intraperitoneally with STZ (150 mg·kg-1 ) and the type 1 diabetic mice were determined with high fasting blood glucose ( >16. 7 mmol · L-1 ) 72 hours after injection. After fed for one month, normal control mice and diabetic mice were sacrificed. The serum TNF-α level was detected by the method of ELISA. Immunohistochemistry and Western blot were used to explore the expression of SREBP-1 and ADRP. Re-sults TheresultsofELISAshowedthatserumTNF-αwas increased by 7 . 73 times in diabetic mice compared with normal mice. SREBP-1 and ADRP expressions were located in renal tubular cells. Again, it was con-firmed by Western blot that SREBP-1 precursor seg-ment, SREBP-1 mature segment and ADRP were re-spectively enhanced by 2. 31 times, 1. 74 times and 1. 72 times in diabetic mice in comparison with normal control mice . In addition , the data analysis revealed a <br> positive correlation ( correlation coefficient 0. 914 ) be-tween serum TNF-αand renal ADRP expression. Con-clusion TheincreasedserumTNF-αmaybeoneof factors involved in renal lipid accumulation of diabe-tes.