CAJ | 학술논문

目的：研究松果菊苷(echinacoside,ECH)对帕金森病(Parkinsonˊsdisease,PD)大鼠纹状体及海马细胞外液中单胺类神经递质的影响,以探讨ECH对脑神经保护作用的可能机制。方法采用双点注射6-羟基多巴胺损毁术制作PD模型,各组大鼠腹腔注射相应的药物或生理盐水连续4周,进行双靶点微透析程序,将透析液注入高效液相-电化学检测器(HPLC-ECD),测定各组大鼠纹状体及海马细胞外液中多巴胺(DA)、3,4-二羟基苯乙酸(DOPAC)、高香草酸(HVA)、去甲肾上腺素(NE)及5-羟色胺(5-HT)的含量。结果与对照组相比,模型组大鼠纹状体及海马细胞外液中NE、DA、DOPAC、HVA、5-HT含量均明显降低(P<0.01)；与模型组相比,各给药组大鼠的纹状体及海马细胞外液中5种物质的含量均明显升高(P<0.05,P<0.01)；ECH高剂量组与阳性药MD组相差不大。结论ECH对PD大鼠纹状体及海马细胞外液中的单胺类神经递质的含量减少具有改善作用,对于PD具有一定的治疗作用,这是ECH对PD大鼠脑神经保护作用的可能机制之一。
목적：연구송과국감(echinacoside,ECH)대파금삼병(Parkinsonˊsdisease,PD)대서문상체급해마세포외액중단알류신경체질적영향,이탐토ECH대뇌신경보호작용적가능궤제。방법채용쌍점주사6-간기다파알손훼술제작PD모형,각조대서복강주사상응적약물혹생리염수련속4주,진행쌍파점미투석정서,장투석액주입고효액상-전화학검측기(HPLC-ECD),측정각조대서문상체급해마세포외액중다파알(DA)、3,4-이간기분을산(DOPAC)、고향초산(HVA)、거갑신상선소(NE)급5-간색알(5-HT)적함량。결과여대조조상비,모형조대서문상체급해마세포외액중NE、DA、DOPAC、HVA、5-HT함량균명현강저(P<0.01)；여모형조상비,각급약조대서적문상체급해마세포외액중5충물질적함량균명현승고(P<0.05,P<0.01)；ECH고제량조여양성약MD조상차불대。결론ECH대PD대서문상체급해마세포외액중적단알류신경체질적함량감소구유개선작용,대우PD구유일정적치료작용,저시ECH대PD대서뇌신경보호작용적가능궤제지일。
Aim Tostudytheeffectsofechinacoside on striatal and hippocampus extracellular fluid of mono-amine neurotransmitter in Parkinsonˊs disease rat, and discuss the possible mechanism of the ECH effect on brainprotection.Methods Doublepointinjectionof 6-hydroxy dopamine damaged was adopted to make PD model, the corresponding drugs or normal saline were intraperitoneally injected for 4 consecutive weeks, mi-cro-dialysis program in double targets was taken after treatment, the dialysate was injected into HPLC-EDC, and striatal and hippocampus extracellular fluid of DA, DOPAC, HVA, NA, 5-HT levels of each group were measured. Results Compared with control group, striatal and hippocampus extracellular fluid of DA, DOPAC, HVA, NE, 5-HT levels were significantly re-duced in model group ( P <0. 01 ); compared with model group, striatal and hippocampus extracellular fluid of DA, DOPAC, HVA, NE, 5-HT levels were significantly increased in treatment group ( P <0 . 05 , P<0. 01 ) , and the content of five kinds of substances in ECH high dose group was similar to that in MD group.Conclusion ECHcouldincreaseextracellular monoamine neurotransmitters in striatum and hippo-campus, ECH has a therapeutic effect on PD, and echinacoside is possible mechanisms of the brain pro-tective effect of PD rats.