CAJ | 학술논문

目的：探讨5-氨基乙酰丙酸(5-ALA )对二甲双胍( Met)降血糖作用的影响及其机制。方法腹腔注射链脲菌素诱导小鼠糖尿病模型,用5-ALA和Met治疗30 d,监测小鼠体征,检测空腹血糖、葡萄糖耐量、血浆胰岛素、瘦素水平,评估胰岛素敏感性,进行肝脏、胰腺组织学检查。结果添加5-ALA的Met组比单用Met组血糖、糖耐量实验的曲线下面积均明显降低；血浆胰岛素、瘦素水平有所下降,胰岛素敏感性改善更为明显。模型组小鼠肝窦周围细胞排列紊乱,可见空泡样变；给药各组均未见此改变。模型组小鼠胰岛数量及细胞胞质较正常组明显减少,胞核固缩；添加5-ALA的各治疗组胰岛数量均较模型组增多,细胞形态趋于正常,且呈现出一定的5-ALA剂量依赖性。结论5-ALA可通过提高胰岛素敏感性及保护肝脏、胰腺的形态与功能,增强Met的降血糖作用。
목적：탐토5-안기을선병산(5-ALA )대이갑쌍고( Met)강혈당작용적영향급기궤제。방법복강주사련뇨균소유도소서당뇨병모형,용5-ALA화Met치료30 d,감측소서체정,검측공복혈당、포도당내량、혈장이도소、수소수평,평고이도소민감성,진행간장、이선조직학검사。결과첨가5-ALA적Met조비단용Met조혈당、당내량실험적곡선하면적균명현강저；혈장이도소、수소수평유소하강,이도소민감성개선경위명현。모형조소서간두주위세포배렬문란,가견공포양변；급약각조균미견차개변。모형조소서이도수량급세포포질교정상조명현감소,포핵고축；첨가5-ALA적각치료조이도수량균교모형조증다,세포형태추우정상,차정현출일정적5-ALA제량의뢰성。결론5-ALA가통과제고이도소민감성급보호간장、이선적형태여공능,증강Met적강혈당작용。
Aim Toexaminethecombinedeffectsof 5-ALA and metformin on diabetes mellitus induced by streptozotocin( STZ) in mice, and to discuss the mech-anisminitially.Methods Thediabeticmiceinduced by intraperitoneal injection of STZ were treated with 5-ALA and/or metformin for 30 days. Physical signs, fasting blood glucose ( FBG ) , fasting plasma insulin ( FIns) , plasma leptin ( Lep) , glucose tolerance, and histological changes of liver and pancreas were as-sessed. Insulin resistance was evaluated by the homeo-stasismodelassessment(HOMA).Results There were more significant effects when 5-ALA combined with metformin than only using metformin on lowering FBG, FIns and Lep. And 5-ALA combined with met-formin could improve glucose tolerance and the insulin sensitivity. Microscopic analysis demonstrated that sig-nificant changes in pancreatic islet and characteristic feature of the vacuolization phenotype in liver was ob-served on diabetic mice, and use of 5-ALA could in-hibit shrinking pancreatic islet number and hepatic morphological changes, especially in combination with metformin.Conclusions Asynergisticeffectof5-ALA and metformin is observed. Combined therapy of 5-ALA and metformin can decrease FBG and improve glucose tolerance, insulin sensitivity and islet ? cells function and morphology.