CAJ | 학술논문

目的本实验应用Micro CT研究洛伐他汀和秋水仙碱对CCl4致肝损伤后小鼠骨代谢的影响,并比较二者的效果。方法体积分数40%的CCl4花生油皮下注射,建立小鼠肝损伤模型,同时,洛伐他汀按2.6 mg·kg-1,秋水仙碱按0.065 mg·kg-1每天1次灌胃给药,连续1个月,于实验结束后观察肝损伤血清学指标以及肝匀浆相关的抗氧化指标,并用Micro CT测定小鼠胫骨骨组织结构参数。结果单用CCl4小鼠肝指数明显增加,AST和ALT活性明显升高,肝匀浆中SOD活性和GSH-Px水平明显降低, MDA水平明显升高,且胫骨上段骨组织结构参数BVF、TMD值明显降低,SMI值明显升高,出现明显的骨质疏松症状。而洛伐他汀、秋水仙碱用药后对肝损伤没有明显影响,但洛伐他汀对肝损伤导致的骨丢失有明显的保护作用,秋水仙碱组则没有明显的保护作用。结论 CCl4致肝损伤后的小鼠出现骨质疏松症状,洛伐他汀用药后对CCl4致肝损伤的骨丢失有明显的预防作用。
목적본실험응용Micro CT연구락벌타정화추수선감대CCl4치간손상후소서골대사적영향,병비교이자적효과。방법체적분수40%적CCl4화생유피하주사,건립소서간손상모형,동시,락벌타정안2.6 mg·kg-1,추수선감안0.065 mg·kg-1매천1차관위급약,련속1개월,우실험결속후관찰간손상혈청학지표이급간균장상관적항양화지표,병용Micro CT측정소서경골골조직결구삼수。결과단용CCl4소서간지수명현증가,AST화ALT활성명현승고,간균장중SOD활성화GSH-Px수평명현강저, MDA수평명현승고,차경골상단골조직결구삼수BVF、TMD치명현강저,SMI치명현승고,출현명현적골질소송증상。이락벌타정、추수선감용약후대간손상몰유명현영향,단락벌타정대간손상도치적골주실유명현적보호작용,추수선감조칙몰유명현적보호작용。결론 CCl4치간손상후적소서출현골질소송증상,락벌타정용약후대CCl4치간손상적골주실유명현적예방작용。
Aim Toinvestigatetheinfluenceoflovas-tatin and colchicine on bone lost of mice with liver in-juryinducedbyCCl4withMicroCT.Methods Mod-el group of liver injury was established by injecting 40% of CCl4 disolved subcutaneously. Drug interven-tion groups were treated with 2 . 6 mg · kg-1 lovastatin and 0. 065 mg·kg-1 colchicine, respevtively, by in-tragastric administration daily and last for 30 days. Se-rum enzyme activities of liver injury index and the antioxidant index of liver homogenate were measured and observed. The parameters of proximal tibia were determinedbyMicroCT.Results Inmodelgroup, the AST, ALT levels in serum and MDA content in liv-er homogenate markedly increased, and SOD, GSH-Px activities in liver homogenate significantly decreased. Moreover, the parameters of BVF, TMD of the proxi-mal tibia were significantly decreased, and SMI in-creased inversely. Both lovastatin and colchicine couldnˊt prevent liver injury. Nevertheless, lovastatin had good protection effect against bone lost in mice withliverinjuryinducedbyCCl4.Conclusion The bone mass is lost in mice with liver injury induced by CCl4 , and lovastatin has good protection effect against bone lost.