CAJ | 학술논문

目的：探讨灵芝多糖联合二甲双胍对2型糖尿病大鼠胸主动脉病变的预防作用及其作用机制。方法 SD大鼠高能量饮食4周后,腹腔注射小剂量链脲佐菌素( STZ)30 mg ·kg-1建立2型糖尿病(type 2 diabetes mellitus,T2DM)大鼠模型。成模后随机分为模型组、灵芝多糖组(灵芝多糖600 mg·kg-1)、二甲双胍组(二甲双胍600 mg·kg-1)及联合用药组(灵芝多糖300 mg · kg-1+二甲双胍300 mg · kg-1),另设正常对照组。给药12周末测定大鼠空腹血糖、血清过氧化氢酶( CAT)、谷胱甘肽过氧化物酶( GSH-Px)、总胆固醇( TC)、甘油三酯( TG)水平；HE染色观察胸主动脉病理学变化；免疫组化、蛋白印记法检测胸主动脉VEGF的表达。结果联合用药组大鼠空腹血糖、血脂水平明显降低,血清CAT、GSH-Px水平明显升高,胸主动脉血管内皮生长因子( VEGF)表达得到抑制,主动脉病理观察显示联合用药组内膜增厚、内皮脂质沉积较模型组少。结论灵芝多糖联合二甲双胍对2型糖尿病大鼠主动脉病变有预防作用,其机制可能与抑制主动脉氧化应激,调节血脂,下调主动脉VEGF的表达有关。
목적：탐토령지다당연합이갑쌍고대2형당뇨병대서흉주동맥병변적예방작용급기작용궤제。방법 SD대서고능량음식4주후,복강주사소제량련뇨좌균소( STZ)30 mg ·kg-1건립2형당뇨병(type 2 diabetes mellitus,T2DM)대서모형。성모후수궤분위모형조、령지다당조(령지다당600 mg·kg-1)、이갑쌍고조(이갑쌍고600 mg·kg-1)급연합용약조(령지다당300 mg · kg-1+이갑쌍고300 mg · kg-1),령설정상대조조。급약12주말측정대서공복혈당、혈청과양화경매( CAT)、곡광감태과양화물매( GSH-Px)、총담고순( TC)、감유삼지( TG)수평；HE염색관찰흉주동맥병이학변화；면역조화、단백인기법검측흉주동맥VEGF적표체。결과연합용약조대서공복혈당、혈지수평명현강저,혈청CAT、GSH-Px수평명현승고,흉주동맥혈관내피생장인자( VEGF)표체득도억제,주동맥병리관찰현시연합용약조내막증후、내피지질침적교모형조소。결론령지다당연합이갑쌍고대2형당뇨병대서주동맥병변유예방작용,기궤제가능여억제주동맥양화응격,조절혈지,하조주동맥VEGF적표체유관。
Aim To investigate the effects and mecha of ganoderma lucidum polysaccharides and metformin on pathological changes of thoracic aorta in diabetic ratsandthemechanisms.Methods SDratswerefed with high fat diet for 4 weeks and injected with strepto-zotocin ( 30 mg · kg-1 ) to replicate type 2 diabetic model. The diabetic rats were randomly into diabetes group, ganoderma lucidum polysaccharides group ( 600 mg·kg-1 ) ,metformin group(600 mg·kg-1 ) ,combi-nation group ( ganoderma lucidum polysaccharides 300 mg· kg-1 + metformin 300 mg · kg-1 ) and normal control group. After 12 weeksˊ treatment, the levels of fasting serum glucose, the activity of catalase(CAT), glutathione peroxidase ( GSH-Px ) , total cholesterol (TC)and triglyceride(TG) in serum were detected. Pathological changes of thoracic aorta were observed by HE staining. Immunohistochemy and Western blot were used to detect thoracic aorta VEGF protein expression. Results Combination group could lower fasting serum glucose and blood fat significantly, meanwhile the ac-tivity of CAT and GSH-Px in serum was improved. The expression of VEGF in thoracic aorta was repressed. The result of HE staining suggested that the lipid de-posits in aortic endothelium in combination group were lessthanthoseinthemodelgroup.Conclusions Ga-noderma lucidum polysaccharides combined with met-formin has an obvious prevention on pathological chan-ges of thoracic aorta in diabetic rats. The possible mechanism may be related to repressing oxidative stress of thoracic aorta, regulating the dyslipidemia, and the down regulation of the expression of VEGF in thoracic aorta.