亚热带植物科学
亞熱帶植物科學
아열대식물과학
SUBTROPICAL PLANT SCIENCE
2014年
3期
255-258
,共4页
王华宇%李丽凤%陈艳%陈乃明
王華宇%李麗鳳%陳豔%陳迺明
왕화우%리려봉%진염%진내명
南天竹%组织培养%快繁%增殖%生根
南天竹%組織培養%快繁%增殖%生根
남천죽%조직배양%쾌번%증식%생근
Nandina domestica%tissue culture%rapid propagation%multiplication%rooting
以火焰南天竹和尖叶南天竹茎段与茎尖为外植体,研究不定芽诱导、增殖和生根技术。结果表明,两品种的最佳增殖培养基均为MS+6-BA 1.0 mg·L-1+NAA 0.1 mg·L-1;在培养基1/2MS+NAA 0.1 mg·L-1+IBA 0.1 mg·L-1中,尖叶南天竹生根率可达100%,而火焰南天竹生根率仅73.5%;将火焰南天竹的生根材料在该生根培养基中接种后培养15 d,再转入经γ射线辐射灭菌的椰糠中,可将生根率提高至92.0%,且植株生长健壮。
以火燄南天竹和尖葉南天竹莖段與莖尖為外植體,研究不定芽誘導、增殖和生根技術。結果錶明,兩品種的最佳增殖培養基均為MS+6-BA 1.0 mg·L-1+NAA 0.1 mg·L-1;在培養基1/2MS+NAA 0.1 mg·L-1+IBA 0.1 mg·L-1中,尖葉南天竹生根率可達100%,而火燄南天竹生根率僅73.5%;將火燄南天竹的生根材料在該生根培養基中接種後培養15 d,再轉入經γ射線輻射滅菌的椰糠中,可將生根率提高至92.0%,且植株生長健壯。
이화염남천죽화첨협남천죽경단여경첨위외식체,연구불정아유도、증식화생근기술。결과표명,량품충적최가증식배양기균위MS+6-BA 1.0 mg·L-1+NAA 0.1 mg·L-1;재배양기1/2MS+NAA 0.1 mg·L-1+IBA 0.1 mg·L-1중,첨협남천죽생근솔가체100%,이화염남천죽생근솔부73.5%;장화염남천죽적생근재료재해생근배양기중접충후배양15 d,재전입경γ사선복사멸균적야강중,가장생근솔제고지92.0%,차식주생장건장。
The stems and stem apexes ofNandina domestica ‘Firepower’ andN. domestica ‘Harbour Dwarf’ were used as explants to study the techniques of adventitious bud induction, proliferation and rooting. The results showed that the best medium for proliferation of two cultivars was MS + 6-BA 1.0 mg·L-1+ NAA 0.1 mg·L-1. The rooting rate ofN. domestica ‘Harbour Dwarf’ could be as high as 100% in rooting medium 1/2MS + NAA 0.1 mg·L-1+ IBA 0.1 mg·L-1, while only 73.5% for the other cultivar. Plantlets in propagation stage were cultivated for 15 days in the root medium, and then transplanted to coconut chaff in plastic containers which were sterilized byγ-ray. In the coconut chaff, the rooting rate of ‘Firepower’ could be increased to 92.0%, and the plantlets grew well.